scholarly journals A microfluidic device for automated, high-speed microinjection of Caenorhabditis elegans

2016 ◽  
Vol 10 (1) ◽  
pp. 011912 ◽  
Author(s):  
Pengfei Song ◽  
Xianke Dong ◽  
Xinyu Liu
Keyword(s):  
Caenorhabditis Elegans ◽  
Microfluidic Device ◽  
High Speed

Advances of Caenorhabditis elegans research on microfluidic device

2016 ◽  
Vol 34 (11) ◽  
pp. 1031
Author(s):  
Fangchao YIN ◽  
Hui WEN ◽  
Guoli ZHU ◽  
Jianhua QIN
Keyword(s):  
Caenorhabditis Elegans ◽  
Microfluidic Device

Generation and Transport of Bubbles in a Microfluidic Device

2008 ◽  
Author(s):  
Renqiang Xiong ◽  
Jacob N. Chung
Keyword(s):  
Silicon Wafer ◽  
Microfluidic Device ◽  
High Speed ◽  
Micro Channel ◽  
Bubble Generation ◽  
Air Bubble ◽  
Micro Scale ◽  
Bubble Transport

In this paper we used high speed recording to characterize segmented micro-scale air bubble generation in a T-junction and bubble transport in a serpentine micro-channel fabricated in a standard silicon wafer.

Development of an integrated microfluidic device for evaluating of in vivo chemo-sensing of intact Caenorhabditis elegans

2013 ◽  
Vol 178 ◽  
pp. 343-349 ◽  
Author(s):  
Jingjing Wang ◽  
Zhaoyu Li ◽  
Zijing Xu ◽  
Liang Hu ◽  
Xiaojun Feng ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Microfluidic Device

scholarly journals High-Speed Discrimination and Sorting of Submicron Particles Using a Microfluidic Device

Nano Letters ◽  
2014 ◽  
Vol 15 (1) ◽  
pp. 469-475 ◽  
Author(s):  
Sukumar Rajauria ◽  
Christopher Axline ◽  
Claudia Gottstein ◽  
Andrew N. Cleland
Keyword(s):  
Microfluidic Device ◽  
High Speed ◽  
Submicron Particles ◽  
Speed Discrimination

scholarly journals Adverse Effects of Hydroalcoholic Extracts and the Major Components in the Stems of Impatiens balsamina L. on Caenorhabditis elegans

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Hong-Fang Jiang ◽  
Zi-Heng Zhuang ◽  
Bei-Wei Hou ◽  
Bao-Jun Shi ◽  
Cheng-Jie Shu ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Adverse Effects ◽  
High Speed ◽  
High Performance ◽  
Negative Impact ◽  
C Elegans ◽  
Impatiens Balsamina ◽  
Annual Herb ◽  
Survival Frequency

Impatiens balsamina L. (Balsaminaceae), an annual herb found throughout China, has been extensively used in traditional Chinese medicine (TCM). However, our knowledge regarding the adverse effects of I. balsamina in vivo is very limited. In this present study, the nematode Caenorhabditis elegans model was employed to fully assess the adverse effects of hydroalcoholic (EtOH 55%) extracts of I. balsamina stems (HAEIBS) in vivo. After exposure to 10 mg/mL HAEIBS, the major organism-level endpoints of C. elegans of percent survival, frequency of head thrash and body bends, and reproduction had decreased by 24%, 30%, and 25%, respectively. The lifespan of C. elegans was also greatly reduced after HAEIBS exposure compared to the controls. The active compounds in HAEIBS were separated using high speed countercurrent chromatograph (HSCCC) and characterized by high performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR). Two compounds, lawsone and 2-methoxy-1,4-naphthoquinone (MNQ), and their adverse effects were then more thoroughly detailed in this study. It was found that lawsone is the major toxin in HAEIBS with a higher toxicity than MNQ in terms of negative impact on C. elegans mortality, locomotion, reproduction, and lifespan. Our data also suggests that the C. elegans model may be useful for assessing the possible toxicity of other Chinese medicines, plant extracts, and/or compounds.

scholarly journals An open source microfluidic sorter for Caenorhabditis nematodes

2019 ◽  
Author(s):  
Nadine Timmermeyer ◽  
Stephen A. Banse ◽  
Joseph H. North ◽  
Matthew Sottile ◽  
Shawn R. Lockery ◽  
...  
Keyword(s):  
Fluid Flow ◽  
Caenorhabditis Elegans ◽  
Aqueous Solutions ◽  
Open Source ◽  
Microfluidic Device ◽  
Biological Samples ◽  
Offspring Production ◽  
Custom Made

Rapid and accurate sorting of biological samples is extremely useful in a wide variety of applications. The model nematode Caenorhabditis elegans lends itself to automated fluid-flow based sorting because of its ability to live in aqueous solutions. Here, we build upon previous developments to construct a microfluidic device capable of sorting individuals based on a variety of characteristics, with a specific application toward differentiating fluorescently marked individuals. We find that our new design generates highly repeatable pools of sorted individuals. In general, there tends to be a tradeoff between precision and speed that can be optimized based on several different factors. Importantly, sorting does not decrease offspring production, and individuals can be sorted multiple times for increased precision. We provide detailed parts lists, schematics and software to allow implementation of these methods in other laboratories. Our results demonstrate that custom-made sorter chips can be a flexible and versatile addition to the nematode experimental toolbox.

scholarly journals Hydrogel-droplet microfluidic platform for high-resolution imaging and sorting of early larval Caenorhabditis elegans

Lab on a Chip ◽  
2015 ◽  
Vol 15 (6) ◽  
pp. 1424-1431 ◽  
Author(s):  
Guillaume Aubry ◽  
Mei Zhan ◽  
Hang Lu
Keyword(s):  
Caenorhabditis Elegans ◽  
High Resolution ◽  
Microfluidic Device ◽  
High Resolution Imaging ◽  
Microfluidic Platform ◽  
C Elegans ◽  
Resolution Imaging

We present a microfluidic device for high-resolution imaging and sorting of early larval C. elegans. The animals are isolated in droplets and temporarily immobilized for imaging using a reversible hydrogel.

scholarly journals Comparing Caenorhabditis elegans gentle and harsh touch response behavior using a multiplexed hydraulic microfluidic device

2017 ◽  
Author(s):  
Patrick D. McClanahan ◽  
Joyce H. Xu ◽  
Christopher Fang-Yen
Keyword(s):  
Caenorhabditis Elegans ◽  
Microfluidic Device ◽  
Receptive Fields ◽  
The Body ◽  
Mechanical Stimuli ◽  
C Elegans ◽  
Response Characteristics ◽  
Touch Receptor Neurons ◽  
Touch Response ◽  
Receptor Neurons

AbstractThe roundworm Caenorhabditis elegans is an important model system for understanding the genetics and physiology of touch. Classical assays for C. elegans touch, which involve manually touching the animal with a probe and observing its response, are limited by their low throughput and qualitative nature. We developed a microfluidic device in which several dozen animals are subject to spatially localized mechanical stimuli with variable amplitude. The device contains 64 sinusoidal channels through which worms crawl, and hydraulic valves that deliver touch stimuli to the worms. We used this assay to characterize the behavioral responses to gentle touch stimuli and the less well studied harsh (nociceptive) touch stimuli. First, we measured the relative response thresholds of gentle and harsh touch. Next, we quantified differences in the receptive fields between wild type worms and a mutant with non-functioning posterior touch receptor neurons. We showed that under gentle touch the receptive field of the anterior touch receptor neurons extends into the posterior half of the body. Finally, we found that the behavioral response to gentle touch does not depend on the locomotion of the animal immediately prior to the stimulus, but does depend on the location of the previous touch. Responses to harsh touch, on the other hand, did not depend on either previous velocity or stimulus location. Differences in gentle and harsh touch response characteristics may reflect the different innervation of the respective mechanosensory cells. Our assay will facilitate studies of mechanosensation, sensory adaptation, and nociception.

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