Determination of the separation efficiencies of a single-stage cryogenic distillation setup to remove krypton out of xenon by using a 83mKr tracer method

2015 ◽  
Vol 86 (11) ◽  
pp. 115104 ◽  
Author(s):  
S. Rosendahl ◽  
E. Brown ◽  
I. Cristescu ◽  
A. Fieguth ◽  
C. Huhmann ◽  
...  
Keyword(s):  
Single Stage ◽  
Tracer Method ◽  
Cryogenic Distillation

Isotopic non-stationary 13C gluconate tracer method for accurate determination of the pentose phosphate pathway split-ratio in Penicillium chrysogenum

2008 ◽  
Vol 10 (3-4) ◽  
pp. 178-186 ◽  
Author(s):  
Zheng Zhao ◽  
Karel Kuijvenhoven ◽  
Cor Ras ◽  
Walter M. van Gulik ◽  
Joseph J. Heijnen ◽  
...  
Keyword(s):  
Pentose Phosphate Pathway ◽  
Penicillium Chrysogenum ◽  
Accurate Determination ◽  
Pentose Phosphate ◽  
Tracer Method ◽  
Split Ratio

Determination of the Pressure of the Barium I‐II Transition with Single‐Stage Piston‐Cylinder Apparatus

1967 ◽  
Vol 38 (12) ◽  
pp. 4557-4564 ◽  
Author(s):  
J. C. Haygarth ◽  
I. C. Getting ◽  
G. C. Kennedy
Keyword(s):  
Single Stage ◽  
Piston Cylinder

Mass isotopomer study of the nonoxidative pathways of the pentose cycle with [1,2-13C2]glucose

1998 ◽  
Vol 274 (5) ◽  
pp. E843-E851 ◽  
Author(s):  
Wai-Nang Paul Lee ◽  
Laszlo G. Boros ◽  
Joaquim Puigjaner ◽  
Sara Bassilian ◽  
Shu Lim ◽  
...  
Keyword(s):  
Pentose Phosphate ◽  
Gas Chromatography Mass Spectrometry ◽  
Hep G2 ◽  
Tracer Method ◽  
Human Hepatoma Cells ◽  
Glucose Flux ◽  
Isotopomer Analysis ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Mass Isotopomer

We present a single-tracer method for the study of the pentose phosphate pathway (PPP) using [1,2-13C2]glucose and mass isotopomer analysis. The metabolism of [1,2-13C2]glucose by the glucose-6-phosphate dehydrogenase, transketolase (TK), and transaldolase (TA) reactions results in unique pentose and lactate isotopomers with either one or two13C substitutions. The distribution of these isotopomers was used to estimate parameters of the PPP using the model of Katz and Rognstad (J. Katz and R. Rognstad. Biochemistry 6: 2227–2247, 1967). Mass and position isotopomers of ribose, and lactate and palmitate (products from triose phosphate) from human hepatoma cells (Hep G2) incubated with 30% enriched [1,2-13C2]glucose were determined using gas chromatography-mass spectrometry. After 24–72 h incubation, 1.9% of lactate molecules in the medium contained one 13C substitution ( m 1) and 10% contained two 13C substitutions ( m 2). A similar m 1-to- m 2ratio was found in palmitate as expected. Pentose cycle (PC) activity determined from incubation with [1,2-13C2]glucose was 5.73 ± 0.52% of the glucose flux, which was identical to the value of PC (5.55 ± 0.73%) determined by separate incubations with [1-13C] and [6-13C]glucose.13C was found to be distributed in four ribose isotopomers ([1-13C]-, [5-13C]-, [1,2-13C2]-, and [4,5-13C2]ribose). The observed ribose isotopomer distribution was best matched with that provided from simulation by substituting 0.032 for TK and 0.85 for TA activity relative to glucose uptake into the model of Katz and Rognstad. The use of [1,2-13C2]glucose not only permits the determination of PC but also allows estimation of relative rates through the TK and TA reactions.

scholarly journals Observability of anammox activity in single-stage nitritation/anammox reactors using mass balances

2015 ◽  
Vol 1 (4) ◽  
pp. 523-534 ◽  
Author(s):  
Sarina Schielke-Jenni ◽  
Kris Villez ◽  
Eberhard Morgenroth ◽  
Kai M. Udert
Keyword(s):  
Heterotrophic Bacteria ◽  
Single Stage ◽  
Anammox Bacteria ◽  
Mass Balances ◽  
Microbial Kinetics ◽  
Anammox Activity

Theoretically, mass balances based on microbial kinetics allow the determination of the activity of anammox bacteria (AMX) and heterotrophic bacteria (HET). In practise, the variance of the resulting activities is too high.

Single-Stage Determination of Argon, Oxygen and Nitrogen in Air

1965 ◽  
Vol 3 (7) ◽  
pp. 242-243 ◽  
Author(s):  
E. L. Obermiller ◽  
R. W. Freedman
Keyword(s):  
Single Stage

Determination of the stability constant for MHL formation by a tracer method

1982 ◽  
Vol 21 (4) ◽  
pp. 1696-1697 ◽  
Author(s):  
S. M. Shanbhag ◽  
G. R. Choppin
Keyword(s):  
Stability Constant ◽  
Tracer Method ◽  
The Stability

Isotopic determination of glycolytic flux during intense exercise in humans

1995 ◽  
Vol 78 (2) ◽  
pp. 483-490 ◽  
Author(s):  
B. D. Williams ◽  
I. Plag ◽  
J. Troup ◽  
R. R. Wolfe
Keyword(s):  
Energy Demand ◽  
Glycolytic Flux ◽  
Tracer Method ◽  
Intense Exercise ◽  
Isotope Tracer ◽  
Exercise Onset ◽  
Highly Correlated ◽  
Exercise In Humans ◽  
O2 Deficit

We used a new stable isotope tracer approach incorporating muscle intracellular lactate enrichment to determine the flux of glucose/glucosyl toward lactate [i.e., nonoxidized pyruvate (Pyr) production (Pyrno)] in moderately trained cyclists exercising at approximately 80% (259 +/- 16 W; n = 6) and approximately 100% (341 +/- 9 W; n = 8) maximal O2 uptake (VO2max). Primed constant infusions of [6,6-2H2]glucose and [13C]lactate or [13C]Pyr tracers were given, and rapid achievement of plateau was obtained during exercise by increasing the infusion rates at exercise onset to correspond with expected increases in production. The accumulated O2 deficit was simultaneously determined over the 1st 3 min of exercise as an indirect means of quantifying glycolytic flux for comparison with our tracer-determined values and was significantly greater at the higher intensity (38 +/- 3 vs. 30 +/- 3 ml O2.kg-1.3 min-1; P < 0.02). Pyrno was also significantly higher (6.38 +/- 0.91 vs. 4.38 +/- 0.65 mmol.kg-1.min-1 over 3 min at 100 and 80% VO2max, respectively). The blood lactate rate of appearance at approximately 100% VO2max (828 +/- 69 mumol.kg-1.min-1) represented a higher percentage of Pyr rate of appearance (RaPyr; 31 +/- 3%) than that at approximately 80% VO2max (416 +/- 36 mumol.kg-1.min-1; 22 +/- 2%; P < 0.02). Although only approximately 27 +/- 2% of RaPyr was oxidized, this provided 78 +/- 2% of the total energy demand during the 1st 3 min of exercise at either intensity. Our new method provided values for Pyrno that were in the expected range and were highly correlated with respective accumulated O2 deficit values (r = 0.87, P < 0.0001). In conclusion, our new tracer method appears to be valid for the measurement of RaPyr and Pyrno during high-intensity exercise lasting even < 10 min.

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