scholarly journals One-step fabrication of 3D silver paste electrodes into microfluidic devices for enhanced droplet-based cell sorting

AIP Advances ◽  
2015 ◽  
Vol 5 (5) ◽  
pp. 057134 ◽  
Author(s):  
Lang Rao ◽  
Bo Cai ◽  
Xiao-Lei Yu ◽  
Shi-Shang Guo ◽  
Wei Liu ◽  
...  
Keyword(s):  
Cell Sorting ◽  
Microfluidic Devices ◽  
Silver Paste ◽  
One Step

Surface Engineering in Microfluidic Devices for the Isolation of Smooth Muscle Cells and Endothelial Cells

2007 ◽  
Vol 1004 ◽  
Author(s):  
Shashi Murthy ◽  
Brian Plouffe ◽  
Milica Radisic
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Shear Stress ◽  
Smooth Muscle ◽  
Cell Adhesion ◽  
Smooth Muscle Cells ◽  
Cell Sorting ◽  
Microfluidic Devices ◽  
Muscle Cells ◽  
Small Sample

AbstractMicrofluidic cell separation systems have emerged as attractive alternatives to traditional techniques in recent years. These systems offer the advantages of being able to handle small sample volumes and at the same time achieve highly selective separation. Conventional separation techniques, including both fluorescence-activated cell sorting (FACS) and magnetic-activated cell sorting (MACS), typically require a pre-processing incubation step to attach ligated tags (such as fluorescent dyes or magnetic beads) to cell surfaces prior to separation. These techniques are also constrained by infrastructure and high cost. Microfluidic devices with surface-immobilized adhesion molecules eliminate the need for pre-processing incubation and are a low cost alternative.We describe the selective adhesion of smooth muscle cells and endothelial cells in microfluidic devices coated with adhesion peptides. The device geometry is such that the shear stress varies linearly as a function of flow channel length, allowing simultaneous evaluation of the effects of surface chemistry and fluid shear on cell adhesion. The adhesion peptides, val-ala-pro-gly (VAPG) and arg-glu-asp-val (REDV), are known to bind selectively to smooth muscle cells and endothelial cells, respectively. These peptides were tethered to the device surface using silane chemistry and NHS-ester coupling. Cell adhesion was examined in a shear stress range of 1.3-4.0 dyn/cm2. Under these conditions, endothelial cells show significantly higher adhesion to REDV-coated devices compared to smooth muscle cells and fibroblasts. Correspondingly, smooth muscle cell adhesion in VAPG-coated devices is much greater than that of endothelial cells and fibroblasts. This selective binding behavior is also observed when mixed suspensions of the three cell types are flowed into both types of peptide-coated microfluidic devices. These results suggest that microfluidic devices coated with REDV and VAPG can be used as effective separation tools in various applications, such as tissue engineering. Specific examples of applications in cardiac and skin tissue engineering will be discussed.

scholarly journals Microfluidic cell sorting: a review of the advances in the separation of cells from debulking to rare cell isolation

Lab on a Chip ◽  
2015 ◽  
Vol 15 (5) ◽  
pp. 1230-1249 ◽  
Author(s):  
C. Wyatt Shields IV ◽  
Catherine D. Reyes ◽  
Gabriel P. López
Keyword(s):  
Cell Sorting ◽  
Microfluidic Devices ◽  
Cell Isolation ◽  
Physical Mechanisms ◽  
The Many

This review surveys the leading approaches for sorting cells in microfluidic devices and organizes those technologies by the many physical mechanisms exploited for sorting.

Optical Manipulation of Inorganic and Organic Objects in Soft Microfluidic Devices

2000 ◽  
Vol 657 ◽  
Author(s):  
Cengiz S. Ozkan ◽  
Erhan Ata ◽  
Mihrimah Ozkan ◽  
Sadik C. Esener
Keyword(s):  
Optical Tweezers ◽  
Cell Sorting ◽  
Cell Biology ◽  
Microfluidic Devices ◽  
Optical Manipulation ◽  
Microfluidic Channels ◽  
Negative Photoresist ◽  
Pdms Elastomer ◽  
Biology Research ◽  
Inorganic And Organic

ABSTRACTWe describe a technique for trapping and manipulation of inorganic and organic objects in microfluidic channels, based on photonic momentum transfer using an optical tweezers arrangement. Microfluidic devices have been fabricated by polydimethylsiloxane (PDMS) elastomer molding of patterns lithographically defined on a thick negative photoresist. Polystyrene microspheres dispersed in water were transferred into the fluidic channels using a syringe pump. Microspheres and live biological cells are trapped and redirected by optical manipulation within the fluidic channels. Optical trapping and patterning will have applications in creation of active cellular arrays for cell biology research, tissue engineering, cell sorting and drug discovery.

A one-step protocol for the chemical derivatisation of glass microfluidic devices

Lab on a Chip ◽  
2006 ◽  
Vol 6 (4) ◽  
pp. 471 ◽  
Author(s):  
Robert C. R. Wootton ◽  
Andrew J. deMello
Keyword(s):  
Microfluidic Devices ◽  
One Step

scholarly journals One-step selective-wettability modification of PMMA microfluidic devices by using controllable gradient UV irradiation (CGUI)

2018 ◽  
Vol 273 ◽  
pp. 1508-1518 ◽  
Author(s):  
Chao Liang ◽  
Yuanchang Liu ◽  
Chong Liu ◽  
Xia Li ◽  
Li Chen ◽  
...  
Keyword(s):  
Uv Irradiation ◽  
Microfluidic Devices ◽  
One Step

One-step photostructuring of multiple hydrogel arrays for compartmentalized enzyme reactions in microfluidic devices

2019 ◽  
Vol 4 (12) ◽  
pp. 2141-2155 ◽  
Author(s):  
Franziska Obst ◽  
David Simon ◽  
Philipp J. Mehner ◽  
Jens W. Neubauer ◽  
Anthony Beck ◽  
...  
Keyword(s):  
Microfluidic Devices ◽  
Enzyme Reactions ◽  
One Step ◽  
Double Chamber

A moulding technique is presented for the simultaneous photostructuring on the μm scale of hydrogels with nanomaterials on one substrate, usable for the fabrication of microfluidic double-chamber reactors.

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