One-step photostructuring of multiple hydrogel arrays for compartmentalized enzyme reactions in microfluidic devices

2019 ◽  
Vol 4 (12) ◽  
pp. 2141-2155 ◽  
Author(s):  
Franziska Obst ◽  
David Simon ◽  
Philipp J. Mehner ◽  
Jens W. Neubauer ◽  
Anthony Beck ◽  
...  
Keyword(s):  
Microfluidic Devices ◽  
Enzyme Reactions ◽  
One Step ◽  
Double Chamber

A moulding technique is presented for the simultaneous photostructuring on the μm scale of hydrogels with nanomaterials on one substrate, usable for the fabrication of microfluidic double-chamber reactors.

scholarly journals One-step fabrication of 3D silver paste electrodes into microfluidic devices for enhanced droplet-based cell sorting

AIP Advances ◽  
2015 ◽  
Vol 5 (5) ◽  
pp. 057134 ◽  
Author(s):  
Lang Rao ◽  
Bo Cai ◽  
Xiao-Lei Yu ◽  
Shi-Shang Guo ◽  
Wei Liu ◽  
...  
Keyword(s):  
Cell Sorting ◽  
Microfluidic Devices ◽  
Silver Paste ◽  
One Step

A one-step protocol for the chemical derivatisation of glass microfluidic devices

Lab on a Chip ◽  
2006 ◽  
Vol 6 (4) ◽  
pp. 471 ◽  
Author(s):  
Robert C. R. Wootton ◽  
Andrew J. deMello
Keyword(s):  
Microfluidic Devices ◽  
One Step

scholarly journals One-step selective-wettability modification of PMMA microfluidic devices by using controllable gradient UV irradiation (CGUI)

2018 ◽  
Vol 273 ◽  
pp. 1508-1518 ◽  
Author(s):  
Chao Liang ◽  
Yuanchang Liu ◽  
Chong Liu ◽  
Xia Li ◽  
Li Chen ◽  
...  
Keyword(s):  
Uv Irradiation ◽  
Microfluidic Devices ◽  
One Step

Let there be chip—towards rapid prototyping of microfluidic devices: one-step manufacturing processes

2011 ◽  
Vol 3 (12) ◽  
pp. 2681 ◽  
Author(s):  
Ansgar Waldbaur ◽  
Holger Rapp ◽  
Kerstin Länge ◽  
Bastian E. Rapp
Keyword(s):  
Rapid Prototyping ◽  
Microfluidic Devices ◽  
Manufacturing Processes ◽  
One Step

Low-Cost Fabrication of Paper-Based Microfluidic Devices by One-Step Plotting

2012 ◽  
Vol 84 (15) ◽  
pp. 6331-6335 ◽  
Author(s):  
Jinfang Nie ◽  
Yun Zhang ◽  
Liwen Lin ◽  
Caibin Zhou ◽  
Shuhuai Li ◽  
...  
Keyword(s):  
Low Cost ◽  
Microfluidic Devices ◽  
One Step

scholarly journals Immobilization Techniques and Integrated Signal Enhancement for POC Nanocolor Microfluidic Devices

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Marlies Schlauf ◽  
Saied Assadollahi ◽  
Roland Palkovits ◽  
Peter Pointl ◽  
Thomas Gerhard Maria Schalkhammer
Keyword(s):  
Microfluidic Devices ◽  
Thin Layers ◽  
Test Device ◽  
Silver Enhancement ◽  
Protein Conjugates ◽  
Enhanced Absorption ◽  
Optical Readout ◽  
Highly Sensitive ◽  
One Step ◽  
Immobilization Techniques

Resonance enhanced absorption (REA) nanocolor microfluidic devices are new promising bioassay platforms, which employ nanoparticle- (NP-) protein conjugates for the immunodetection of medically relevant markers in biologic samples such as blood, urine, and saliva. The core component of a REA test device is a PET chip coated with aluminum and SiO2thin layers, onto which biorecognitive molecules are immobilized. Upon addition of a sample containing the analyte of interest, a NP-protein-analyte complex is formed in the test device that is captured on the REA chip, for example, via streptavidin-biotin interaction. Thereby, a colored symbol is generated, which allows optical readout. Silver enhancement of the bound nanoparticles may be used to increase the sensitivity of the assay. Herein, we demonstrate that adsorptive immobilization via a cationic polymeric interlayer is a competitive and fast technique for the binding of the capture protein streptavidin onto planar SiO2surfaces such as REA biochips. Moreover, we report the development of a silver enhancement technology that operates even in the presence of high chloride concentrations as may be encountered in biologic samples. The silver enhancement reagents may be integrated into the microfluidic assay platform to be released upon sample addition. Hereby, a highly sensitive one-step assay can be realized.

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