scholarly journals Core-shell hydrogel beads with extracellular matrix for tumor spheroid formation

2015 ◽  
Vol 9 (2) ◽  
pp. 024118 ◽  
Author(s):  
L. Yu ◽  
S. M. Grist ◽  
S. S. Nasseri ◽  
E. Cheng ◽  
Y.-C. E. Hwang ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Core Shell ◽  
Tumor Spheroid ◽  
Spheroid Formation ◽  
Hydrogel Beads

One-Step Dip-Coating-Fabricated Core–Shell Silk Fibroin Rice Paper Fibrous Scaffolds for 3D Tumor Spheroid Formation

2020 ◽  
Vol 3 (11) ◽  
pp. 7462-7471
Author(s):  
Jingjing Fu ◽  
Xiao Bai Li ◽  
Lin Xiang Wang ◽  
Xiao Hui Lv ◽  
Zhisong Lu ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Dip Coating ◽  
Core Shell ◽  
Tumor Spheroid ◽  
Spheroid Formation ◽  
Fibrous Scaffolds ◽  
One Step

Mixed hydrogel bead-based tumor spheroid formation and anticancer drug testing

The Analyst ◽  
2014 ◽  
Vol 139 (10) ◽  
pp. 2449-2458 ◽  
Author(s):  
Yaolei Wang ◽  
Jinyi Wang
Keyword(s):  
Anticancer Drug ◽  
Drug Testing ◽  
Tumor Spheroid ◽  
Spheroid Formation ◽  
Tumor Spheroids ◽  
Hydrogel Bead ◽  
Hydrogel Beads

A microfluidic method was developed for the formation of tumor spheroids using alginate and matrigel mixed hydrogel beads.

Fabrication of PNIPAm-based Thermoresponsive hydrogel microwell arrays for tumor spheroid formation

2021 ◽  
pp. 112100
Author(s):  
Dinesh Dhamecha ◽  
Duong Le ◽  
Tomali Chakravarty ◽  
Kalindu Perera ◽  
Arnob Dutta ◽  
...  
Keyword(s):  
Tumor Spheroid ◽  
Spheroid Formation ◽  
Thermoresponsive Hydrogel

scholarly journals Multicellular tumor spheroid formation in duplex microcapsules for analysis of chemosensitivity

2012 ◽  
Vol 103 (3) ◽  
pp. 549-554 ◽  
Author(s):  
Shinji Sakai ◽  
Kazuya Inamoto ◽  
Yang Liu ◽  
Shinji Tanaka ◽  
Shigeki Arii ◽  
...  
Keyword(s):  
Tumor Spheroid ◽  
Spheroid Formation ◽  
Multicellular Tumor Spheroid

Self-filling microwell arrays (SFMAs) for tumor spheroid formation

Lab on a Chip ◽  
2018 ◽  
Vol 18 (22) ◽  
pp. 3516-3528 ◽  
Author(s):  
Amir Seyfoori ◽  
Ehsan Samiei ◽  
Neda Jalili ◽  
Brent Godau ◽  
Mehdi Rahmanian ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Disease Modeling ◽  
Great Promise ◽  
Tumor Spheroid ◽  
Spheroid Formation ◽  
Tumor Spheroids

Self-filling microwell arrays hold great promise for the production of 3D tumor spheroids and organoids for disease modeling and drug discovery.

A multicellular tumor spheroid formation and extraction chip

2010 ◽  
Author(s):  
Hye-Jin Jin ◽  
Taeyoon Kim ◽  
Young-Ho Cho ◽  
Jin-Mo Gu ◽  
Jhingook Kim ◽  
...  
Keyword(s):  
Tumor Spheroid ◽  
Spheroid Formation ◽  
Multicellular Tumor Spheroid

scholarly journals High Folic Acid Supplementation Reprograms Glycolytic and Lactate-generating Metabolism to Promote Anchorage-independent Tumor Spheroid Formation in NSCLC Cells (P05-001-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Yi-Wen Chen ◽  
Rwei-Fen S Huang
Keyword(s):  
Folic Acid ◽  
Protein Expression ◽  
Glucose Transporter ◽  
Cell Protein ◽  
Control Group ◽  
Tumor Spheroid ◽  
Related Protein ◽  
Spheroid Formation ◽  
Tumor Spheroids ◽  
Glucose Transporter 1

Abstract Objectives Aims of this study were to investigate the effect of HF on anchorage-independent tumor spheroid formation and its’ working mechanisms of lung malignant tumor cells. Methods Human NSCLC cells A549 were cultured in control (C) medium (2.2 μM folic acid) or HF medium (10, 30, 50 μM folic acid) for 4 days. Cells were harvested to explore the self-renewal capacity of cancer cells by observing the anchorage-independent tumor spheroid formation. Meanwhile, the L-lactate assay were conducted to evaluate the lactate-generating state and the cell protein extract for the western blotting analysis to realize expression of energy metabolism related proteins in cells. Results The results showed that numbers of tumor spheroids in HF group were significantly higher than the control group. Besides, compared to C group, HF50 group showed significantly reduced lactate release into medium with highly accumulation in cellular lactate levels. The glycolytic-related protein expression of hexokinase II (HKII), lactate dehydrogenase (LDH) were increased and glucose transporter 1 (GLUT1), pyruvate dehydrogenase (PDH) were decreased in HF group. The signaling-related protein expression of insulin receptor substract-1 (IRS-1), hypoxia-inducible factor-1 alpha (HIF-1α) were increased and PI3 kinase (PI3K), AMP-activated protein kinase (AMPK) were decreased in HF group. Conclusions Collectively, HF supplementation may reprogram glycolytic metabolism and increase anchorage-independent tumor spheroids formation to mediate malignant progress of NSCLC. Funding Sources Ministry of Science and Technology, Taiwan, R.O.C.

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