Mixed hydrogel bead-based tumor spheroid formation and anticancer drug testing

The Analyst ◽  
2014 ◽  
Vol 139 (10) ◽  
pp. 2449-2458 ◽  
Author(s):  
Yaolei Wang ◽  
Jinyi Wang
Keyword(s):  
Anticancer Drug ◽  
Drug Testing ◽  
Tumor Spheroid ◽  
Spheroid Formation ◽  
Tumor Spheroids ◽  
Hydrogel Bead ◽  
Hydrogel Beads

A microfluidic method was developed for the formation of tumor spheroids using alginate and matrigel mixed hydrogel beads.

Self-filling microwell arrays (SFMAs) for tumor spheroid formation

Lab on a Chip ◽  
2018 ◽  
Vol 18 (22) ◽  
pp. 3516-3528 ◽  
Author(s):  
Amir Seyfoori ◽  
Ehsan Samiei ◽  
Neda Jalili ◽  
Brent Godau ◽  
Mehdi Rahmanian ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Disease Modeling ◽  
Great Promise ◽  
Tumor Spheroid ◽  
Spheroid Formation ◽  
Tumor Spheroids

Self-filling microwell arrays hold great promise for the production of 3D tumor spheroids and organoids for disease modeling and drug discovery.

scholarly journals High Folic Acid Supplementation Reprograms Glycolytic and Lactate-generating Metabolism to Promote Anchorage-independent Tumor Spheroid Formation in NSCLC Cells (P05-001-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Yi-Wen Chen ◽  
Rwei-Fen S Huang
Keyword(s):  
Folic Acid ◽  
Protein Expression ◽  
Glucose Transporter ◽  
Cell Protein ◽  
Control Group ◽  
Tumor Spheroid ◽  
Related Protein ◽  
Spheroid Formation ◽  
Tumor Spheroids ◽  
Glucose Transporter 1

Abstract Objectives Aims of this study were to investigate the effect of HF on anchorage-independent tumor spheroid formation and its’ working mechanisms of lung malignant tumor cells. Methods Human NSCLC cells A549 were cultured in control (C) medium (2.2 μM folic acid) or HF medium (10, 30, 50 μM folic acid) for 4 days. Cells were harvested to explore the self-renewal capacity of cancer cells by observing the anchorage-independent tumor spheroid formation. Meanwhile, the L-lactate assay were conducted to evaluate the lactate-generating state and the cell protein extract for the western blotting analysis to realize expression of energy metabolism related proteins in cells. Results The results showed that numbers of tumor spheroids in HF group were significantly higher than the control group. Besides, compared to C group, HF50 group showed significantly reduced lactate release into medium with highly accumulation in cellular lactate levels. The glycolytic-related protein expression of hexokinase II (HKII), lactate dehydrogenase (LDH) were increased and glucose transporter 1 (GLUT1), pyruvate dehydrogenase (PDH) were decreased in HF group. The signaling-related protein expression of insulin receptor substract-1 (IRS-1), hypoxia-inducible factor-1 alpha (HIF-1α) were increased and PI3 kinase (PI3K), AMP-activated protein kinase (AMPK) were decreased in HF group. Conclusions Collectively, HF supplementation may reprogram glycolytic metabolism and increase anchorage-independent tumor spheroids formation to mediate malignant progress of NSCLC. Funding Sources Ministry of Science and Technology, Taiwan, R.O.C.

scholarly journals A 3D Printed Hanging Drop Dripper for Tumor Spheroids Analysis Without Recovery

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Liang Zhao ◽  
Jidong Xiu ◽  
Yang Liu ◽  
Tianye Zhang ◽  
Wenjie Pan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Testing ◽  
Epithelial Mesenchymal Transition ◽  
Three Dimensional ◽  
Tumor Spheroid ◽  
Hanging Drop ◽  
Tumor Spheroids ◽  
Mesenchymal Transition ◽  
Malignant Breast

AbstractCompared with traditional monolayer cell culture, the three-dimensional tumor spheroid has emerged as an essential in vitro model for cancer research due to the recapitulation of the architecture and physiology of solid human tumors. Herein, by implementing the rapid prototyping of a benchtop 3D printer, we developed a new strategy to generate and analyze tumor spheroids on a commonly used multi-well plate. In this method, the printed artifact can be directly mounted on a 96/384-well plate, enables hanging drop-based spheroid formation, avoiding the tedious fabrication process from micromechanical systems. Besides long-term spheroid culture (20 days), this method supports subsequent analysis of tumor spheroid by seamlessly dripping from the printed array, thereby eliminating the need for spheroids retrieval for downstream characterization. We demonstrated several tumor spheroid-based assays, including tumoroid drug testing, metastasis on or inside extracellular matrix gel, and tumor transendothelial (TEM) assay. Based on quantitative phenotypical and molecular analysis without any precarious retrieval and transfer, we found that the malignant breast cancer (MDA-MB-231) cell aggregate presents a more metastatic morphological phenotype than the non-malignant breast cancer (MCF-7) and colonial cancer (HCT-116) cell spheroid, and shows an up-regulation of epithelial-mesenchymal transition (EMT) relevant genes (fold change > 2). Finally, we validated this tumor malignancy by the TEM assay, which could be easily performed using our approach. This methodology could provide a useful workflow for expediting tumoroid modeled in vitro assay, allowing the “Lab-on-a-Cloud” scenario for routine study.

Formation of multicellular tumor spheroids induced by cyclic RGD-peptides and use for anticancer drug testing in vitro

2016 ◽  
Vol 506 (1-2) ◽  
pp. 148-157 ◽  
Author(s):  
Roman Akasov ◽  
Daria Zaytseva-Zotova ◽  
Sergey Burov ◽  
Maria Leko ◽  
Monique Dontenwill ◽  
...  
Keyword(s):  
Anticancer Drug ◽  
Drug Testing ◽  
Multicellular Tumor Spheroids ◽  
Tumor Spheroids ◽  
Rgd Peptides

scholarly journals Core-shell hydrogel beads with extracellular matrix for tumor spheroid formation

2015 ◽  
Vol 9 (2) ◽  
pp. 024118 ◽  
Author(s):  
L. Yu ◽  
S. M. Grist ◽  
S. S. Nasseri ◽  
E. Cheng ◽  
Y.-C. E. Hwang ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Core Shell ◽  
Tumor Spheroid ◽  
Spheroid Formation ◽  
Hydrogel Beads

Fabrication of PNIPAm-based Thermoresponsive hydrogel microwell arrays for tumor spheroid formation

2021 ◽  
pp. 112100
Author(s):  
Dinesh Dhamecha ◽  
Duong Le ◽  
Tomali Chakravarty ◽  
Kalindu Perera ◽  
Arnob Dutta ◽  
...  
Keyword(s):  
Tumor Spheroid ◽  
Spheroid Formation ◽  
Thermoresponsive Hydrogel

Enhanced floating and intragastric release of 5-Flourouracil through sesame oil entrapped gellan composite hydrogel beads

2021 ◽  
Vol 16 ◽  
Author(s):  
Madhurima Das ◽  
Balaram Ghosh ◽  
Tapan Kumar Giri
Keyword(s):  
Drug Transport ◽  
Entrapment Efficiency ◽  
Sesame Oil ◽  
Gellan Gum ◽  
Hydrogel Bead ◽  
Hydrogel Beads ◽  
Buffer Solutions ◽  
Molecular Dispersion ◽  
Time Period

Background: 5-Fluorouracil is an anti-metabolite compound used for several years as an anti-tumor drug. The development of a gastroretentive drug delivery system of 5-Fluorouracil may have advantages since they retain in the stomach for an extended time and release the drug in a sustained manner which ultimately enhances the absorption of the drug and consequently the bioavailability. Objective: The objective of the present work was to prepare a sesame oil-entrapped gellan gum hydrogel bead for controlled stomach specific delivery of 5-Fluorouracil. Methods: Sesame oil-entrapped gellan gum hydrogel bead was prepared by ionotropic gelation method. The developed hydrogels were characterized by SEM, FTIR, DSC, and XRD. The entrapment efficiency, floating ability, swelling and drug release in vitro were also determined. Results: Electrostatic interaction between the carboxylic group of polymers and Ca++ was confirmed by FTIR analysis. The SEM photograph of the hydrogel beads portrayed an approximately spherical shaped structure. DSC thermogram and XRD spectra exhibited the molecular dispersion of the drug inside the hydrogel beads. The developed beads of 5-Fluorouracil floated in pH 1.2 buffer solutions for a prolonged time period and the duration of floating was improved significantly with increasing the concentration of oil. The developed formulations showed controlled release of drug and incorporation of oil retarded the release of the drug. Fickian and non-Fickian mechanism of drug transport was observed from the prepared hydrogel beads. Conclusion: Overall, the oil-entrapped gellan matrices could be used for the intragastric delivery of 5-Fluorouracil to treat stomach cancer.

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