Solid optical ring interferometer for high-throughput feedback-free spectral analysis and filtering

2015 ◽  
Vol 86 (2) ◽  
pp. 023104 ◽  
Author(s):  
B. Petrak ◽  
M. Peiris ◽  
A. Muller
Keyword(s):  
Spectral Analysis ◽  
High Throughput ◽  
Ring Interferometer ◽  
Optical Ring

scholarly journals A modified FASP protocol for high-throughput preparation of protein samples for mass spectrometry

2016 ◽  
Author(s):  
Jeremy Potriquet ◽  
Marut Laohaviroj ◽  
Jeffery Bethony ◽  
Jason Mulvenna
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Spectral Analysis ◽  
High Throughput ◽  
Efficient Method ◽  
Work Flow ◽  
Mass Spectral Analysis ◽  
Mass Spectral ◽  
Molecular Weight Cutoff ◽  
Cellulose Membranes

To facilitate high-throughput proteomic analyses we have developed a modified FASP protocol which improves the rate at which protein samples can be processed prior to mass spectrometry. Adapting the original FASP protocol to a 96-well format necessitates extended spin times for buffer exchange due to the low centrifugation speeds tolerated by these devices. However, by using 96-well plates with a more robust polyethersulfone molecular weight cutoff membrane, instead of the cellulose membranes typically used in these devices, we could use isopropanol as a wetting agent, decreasing spin times required for buffer exchange from an hour to 30 minutes. In a typical work flow used in our laboratory this equates to a reduction of 3 hours per plate. To test whether our modified protocol produced similar results to FASP and other FASP-like protocols we compared the performance of our modified protocol to the original FASP and the more recently described eFASP and MStern-blot. We show that all FASP-like methods, including our modified protocol, display similar performance in terms of proteins identified and reproducibility. Our results show that our modified FASP protocol is an efficient method for the high-throughput processing of protein samples for mass spectral analysis.

154: Integration of TPSA and High-Throughput Mass Spectrometry Data Improves Prostate Cancer Prediction

2007 ◽  
Vol 177 (4S) ◽  
pp. 52-53
Author(s):  
Stefano Ongarello ◽  
Eberhard Steiner ◽  
Regina Achleitner ◽  
Isabel Feuerstein ◽  
Birgit Stenzel ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mass Spectrometry ◽  
High Throughput ◽  
Mass Spectrometry Data ◽  
Cancer Prediction
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