Procedure for the isolation of mitochondria, cytosolic and nuclear material from a single piece of neurological tissue for high-throughput mass spectral analysis

2011 ◽  
Vol 197 (2) ◽  
pp. 279-282 ◽  
Author(s):  
Michael D. Timmons ◽  
Melissa A. Bradley ◽  
Mark A. Lovell ◽  
Bert C. Lynn
Keyword(s):  
Spectral Analysis ◽  
High Throughput ◽  
Nuclear Material ◽  
Mass Spectral Analysis ◽  
Mass Spectral ◽  
Single Piece

scholarly journals A modified FASP protocol for high-throughput preparation of protein samples for mass spectrometry

2016 ◽  
Author(s):  
Jeremy Potriquet ◽  
Marut Laohaviroj ◽  
Jeffery Bethony ◽  
Jason Mulvenna
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Spectral Analysis ◽  
High Throughput ◽  
Efficient Method ◽  
Work Flow ◽  
Mass Spectral Analysis ◽  
Mass Spectral ◽  
Molecular Weight Cutoff ◽  
Cellulose Membranes

To facilitate high-throughput proteomic analyses we have developed a modified FASP protocol which improves the rate at which protein samples can be processed prior to mass spectrometry. Adapting the original FASP protocol to a 96-well format necessitates extended spin times for buffer exchange due to the low centrifugation speeds tolerated by these devices. However, by using 96-well plates with a more robust polyethersulfone molecular weight cutoff membrane, instead of the cellulose membranes typically used in these devices, we could use isopropanol as a wetting agent, decreasing spin times required for buffer exchange from an hour to 30 minutes. In a typical work flow used in our laboratory this equates to a reduction of 3 hours per plate. To test whether our modified protocol produced similar results to FASP and other FASP-like protocols we compared the performance of our modified protocol to the original FASP and the more recently described eFASP and MStern-blot. We show that all FASP-like methods, including our modified protocol, display similar performance in terms of proteins identified and reproducibility. Our results show that our modified FASP protocol is an efficient method for the high-throughput processing of protein samples for mass spectral analysis.

scholarly journals SYNTHESIS, CHARACTERIZATION, AND ANTHELMINTIC ACTIVITY OF NOVEL BENZOTHIAZOLE DERIVATIVES CONTAINING INDOLE MOIETIES

2019 ◽  
pp. 321-325
Author(s):  
ALETI RAJAREDDY ◽  
SRINIVAS MURTHY M
Keyword(s):  
Spectral Analysis ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Magnetic Resonance ◽  
Fourier Transform Infrared Spectroscopy ◽  
Anthelmintic Activity ◽  
Mass Spectral Analysis ◽  
Standard Drug ◽  
Mass Spectral ◽  
Benzothiazole Derivatives

Objective: The objective of this study was to synthesize and evaluate the anthelmintic activity (AA) of novel benzothiazole derivatives containing indole moieties (BDIM). Methods: The present works which involve the substituted isatin Schiff bases undergo acetylating and reacting with 2-aminobenzothiazole to give novel BDIM. Results: All the newly synthesized molecules (5a-5o) were characterized by Fourier-transform infrared spectroscopy, H_nuclear magnetic resonance, and mass spectral analysis along with physical data. The biological potentials of the newly synthesized compounds are evaluated for their AA using an Indian earthworm (Pheretima posthuma), and albendazole was used as standard drug. Conclusion: The synthesized compound 5f, 5n, and 5o showed good AA, whereas others exhibited significant activities.

Large-scale mass spectral analysis by simplex pattern recognition

1976 ◽  
Vol 48 (12) ◽  
pp. 1768-1774 ◽  
Author(s):  
T. Fai. Lam ◽  
Charles L. Wilkins ◽  
Thomas R. Brunner ◽  
Leonard J. Soltzberg ◽  
Steven L. Kaberline
Keyword(s):  
Pattern Recognition ◽  
Spectral Analysis ◽  
Large Scale ◽  
Mass Spectral Analysis ◽  
Mass Spectral
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