Structural information from two-dimensional fifth-order Raman spectroscopy

1999 ◽  
Vol 111 (2) ◽  
pp. 492-503 ◽  
Author(s):  
Ko Okumura ◽  
Andrei Tokmakoff ◽  
Yoshitaka Tanimura
Keyword(s):  
Raman Spectroscopy ◽  
Structural Information ◽  
Two Dimensional ◽  
Fifth Order

Two-dimensional fifth-order Raman spectroscopy of liquid formamide: Experiment and Theory

2008 ◽  
Vol 128 (23) ◽  
pp. 234507 ◽  
Author(s):  
Y. L. Li ◽  
L. Huang ◽  
R. J. Dwayne Miller ◽  
Taisuke Hasegawa ◽  
Yoshitaka Tanimura
Keyword(s):  
Raman Spectroscopy ◽  
Two Dimensional ◽  
Fifth Order

Two Dimensional Fifth-Order Raman Spectroscopy

2008 ◽  
pp. 1-72 ◽  
Author(s):  
Christopher J. Milne ◽  
Yun-Liang Li ◽  
R. J. Dwayne Miller
Keyword(s):  
Raman Spectroscopy ◽  
Two Dimensional ◽  
Fifth Order

Fifth-order two-dimensional Raman spectroscopy: A new direct probe of the liquid state

2003 ◽  
Vol 22 (3) ◽  
pp. 497-532 ◽  
Author(s):  
Kevin J. Kubarych ◽  
Chris J. Milne ◽  
R. J. Dwayne Miller
Keyword(s):  
Raman Spectroscopy ◽  
Liquid State ◽  
Two Dimensional ◽  
Fifth Order

Two-Dimensional Crystallization of Tetanus Toxin

1985 ◽  
Vol 43 ◽  
pp. 528-529
Author(s):  
Jeffry A. Reidler ◽  
John P. Robinson
Keyword(s):  
Electron Microscopy ◽  
Tetanus Toxin ◽  
Structural Information ◽  
Three Dimensional ◽  
Two Dimensional ◽  
Membrane Interface ◽  
3D Reconstructions ◽  
Cellular Receptors ◽  
Computer Reconstruction ◽  
2D Crystals

We have prepared two-dimensional (2D) crystals of tetanus toxin using procedures developed by Uzgiris and Kornberg for the directed production of 2D crystals of monoclonal antibodies at an antigen-phospholipid monolayer interface. The tetanus toxin crystals were formed using a small mole fraction of the natural receptor, GT1, incorporated into phosphatidyl choline monolayers. The crystals formed at low concentration overnight. Two dimensional crystals of this type are particularly useful for structure determination using electron microscopy and computer image refinement. Three dimensional (3D) structural information can be derived from these crystals by computer reconstruction of photographs of toxin crystals taken at different tilt angles. Such 3D reconstructions may help elucidate the mechanism of entry of the enzymatic subunit of toxins into cells, particularly since these crystals form directly on a membrane interface at similar concentrations of ganglioside GT1 to the natural cellular receptors.

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