scholarly journals Cell-free layer (CFL) analysis in a glass capillary: Comparison between a manual and automatic method

2012 ◽  
Author(s):  
David Bento ◽  
Diana Pinho ◽  
Ana I. Pereira ◽  
Rui Lima
Keyword(s):  
Automatic Method ◽  
Free Layer ◽  
Glass Capillary ◽  
Cell Free Layer

scholarly journals Manual and Automatic Image Analysis Segmentation Methods for Blood Flow Studies in Microchannels

Micromachines ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 317
Author(s):  
Violeta Carvalho ◽  
Inês M. Gonçalves ◽  
Andrews Souza ◽  
Maria S. Souza ◽  
David Bento ◽  
...  
Keyword(s):  
Blood Flow ◽  
Image Analysis ◽  
High Speed ◽  
Automatic Image Analysis ◽  
Free Layer ◽  
Glass Capillary ◽  
Analysis Methods ◽  
Segmentation Methods ◽  
Automatic Methods ◽  
Cell Free Layer

In blood flow studies, image analysis plays an extremely important role to examine raw data obtained by high-speed video microscopy systems. This work shows different ways to process the images which contain various blood phenomena happening in microfluidic devices and in microcirculation. For this purpose, the current methods used for tracking red blood cells (RBCs) flowing through a glass capillary and techniques to measure the cell-free layer thickness in different kinds of microchannels will be presented. Most of the past blood flow experimental data have been collected and analyzed by means of manual methods, that can be extremely reliable, but they are highly time-consuming, user-intensive, repetitive, and the results can be subjective to user-induced errors. For this reason, it is crucial to develop image analysis methods able to obtain the data automatically. Concerning automatic image analysis methods for individual RBCs tracking and to measure the well known microfluidic phenomena cell-free layer, two developed methods are presented and discussed in order to demonstrate their feasibility to obtain accurate data acquisition in such studies. Additionally, a comparison analysis between manual and automatic methods was performed.

scholarly journals Emergent cell-free layer asymmetry and biased haematocrit partition in a biomimetic vascular network of successive bifurcations

Soft Matter ◽  
2021 ◽  
Author(s):  
Qi Zhou ◽  
Joana Fidalgo ◽  
Miguel Bernabeu ◽  
Mónica S.N. Oliveira ◽  
Timm Krüger
Keyword(s):  
Red Blood Cells ◽  
Human Body ◽  
Blood Cells ◽  
Soft Matter ◽  
Vascular Network ◽  
Free Layer ◽  
Cell Free Layer

Blood is a vital soft matter, and its normal circulation in the human body relies on the distribution of red blood cells (RBCs) at successive bifurcations. Understanding how RBCs are...

scholarly journals Effect of Cell-Free Layer Variation on Arteriolar Wall Shear Stress

2010 ◽  
Vol 39 (1) ◽  
pp. 359-366 ◽  
Author(s):  
Bumseok Namgung ◽  
Peng Kai Ong ◽  
Paul C. Johnson ◽  
Sangho Kim
Keyword(s):  
Shear Stress ◽  
Wall Shear Stress ◽  
Free Layer ◽  
Arteriolar Wall ◽  
Wall Shear ◽  
Cell Free Layer

scholarly journals Visualization and Quantification of the Cell-free Layer in Arterioles of the Rat Cremaster Muscle

10.3791/54550 ◽  
2016 ◽  
Author(s):  
Yan Cheng Ng ◽  
Liam K. Fisher ◽  
Veena Salim ◽  
Sangho Kim ◽  
Bumseok Namgung
Keyword(s):  
Cremaster Muscle ◽  
Free Layer ◽  
Cell Free Layer

Quantitative evaluation of flow dynamics of erythrocytes in microvessels: influence of erythrocyte aggregation

1995 ◽  
Vol 268 (5) ◽  
pp. H1959-H1965 ◽  
Author(s):  
M. Soutani ◽  
Y. Suzuki ◽  
N. Tateishi ◽  
N. Maeda
Keyword(s):  
Flow Resistance ◽  
Circulatory System ◽  
Flow Dynamics ◽  
Erythrocyte Aggregation ◽  
Intensity Change ◽  
Image Analyzer ◽  
Free Layer ◽  
High Shear Rates ◽  
Suspension Viscosity ◽  
Cell Free Layer

Effects of erythrocyte aggregation on the flow dynamics of erythrocytes in microvessels were examined quantitatively by perfusing human erythrocytes suspended in isotonic medium containing various concentrations of dextran (70,400 avg mol wt, Dx-70) into a part of the microvascular bed isolated from rabbit mesentery. Thickness of the marginal cell-free layer was measured with an image analyzer, total flow resistance was determined on the basis of the perfusion pressure-volume flow relationship, and homogeneity of erythrocyte flow was evaluated by the power spectrum obtained by the fast Fourier transform of the light intensity change monitored on single microvessels. With increasing dextran concentration, suspension viscosity of erythrocytes at high shear rates increased linearly and thickness of the cell-free layer increased in a sigmoidal fashion. Flow resistance increased relatively little over the range of dextran concentrations in which the cell-free layer increased most rapidly. Furthermore, the flow pattern of erythrocytes in microvessels became inhomogeneous. In conclusion, the present study shows that Dx-70-induced erythrocyte aggregation results in increased flow resistance in the circulatory system, even through the widening of the cell-free layer tends to reduce the resistance and also results in inhomogeneous flow of erythrocytes in microvessels.

scholarly journals Effects of cell‐free layer width and its variability on wall shear stress in arterioles

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
BumSeok Namgung ◽  
Peng Kai Ong ◽  
Paul C Johnson ◽  
Sangho Kim
Keyword(s):  
Shear Stress ◽  
Wall Shear Stress ◽  
Free Layer ◽  
Layer Width ◽  
Wall Shear ◽  
Cell Free Layer

Integration of cardiovascular regulation by the blood/endothelium cell-free layer

2011 ◽  
Vol 3 (4) ◽  
pp. 458-470 ◽  
Author(s):  
C. Makena Hightower ◽  
Beatriz Y. Salazar Vázquez ◽  
Sung Woo Park ◽  
Krishna Sriram ◽  
Judith Martini ◽  
...  
Keyword(s):  
Cardiovascular Regulation ◽  
Free Layer ◽  
Cell Free Layer

scholarly journals Cell-free layer analysis in a polydimethysiloxane microchannel: a global approach

2016 ◽  
Vol 8 (3) ◽  
pp. 196 ◽  
Author(s):  
Elmano Pinto ◽  
Vera Faustino ◽  
Diana Pinho ◽  
Raquel O. Rodrigues ◽  
Rui A. Lima ◽  
...  
Keyword(s):  
Global Approach ◽  
Free Layer ◽  
Layer Analysis ◽  
Cell Free Layer
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