Two‐dimensional exchange nuclear magnetic resonance of powder samples. IV. Distribution of correlation times and line shapes in the intermediate dynamic range

1992 ◽  
Vol 97 (11) ◽  
pp. 7944-7954 ◽  
Author(s):  
D. Schaefer ◽  
H. W. Spiess
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Dynamic Range ◽  
Two Dimensional ◽  
Correlation Times ◽  
Line Shapes ◽  
Powder Samples ◽  
Nuclear Magnetic

Rotational diffusion measurements of suspended colloidal particles using two‐dimensional exchange nuclear magnetic resonance

1996 ◽  
Vol 104 (2) ◽  
pp. 509-520 ◽  
Author(s):  
G. A. Barrall ◽  
K. Schmidt‐Rohr ◽  
Y. K. Lee ◽  
K. Landfester ◽  
H. Zimmermann ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Colloidal Particles ◽  
Rotational Diffusion ◽  
Two Dimensional ◽  
Nuclear Magnetic

Anesthetic-membrane interaction: A 2H nuclear magnetic resonance study of the binding of specifically deuterated tetracaine and procaine to phosphatidylcholine

1984 ◽  
Vol 62 (2-3) ◽  
pp. 178-184 ◽  
Author(s):  
Eric C. Kelusky ◽  
Ian C. P. Smith
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Local Anesthetics ◽  
Nuclear Magnetic Resonance Study ◽  
Weakly Bound ◽  
Slow Exchange ◽  
H Nmr ◽  
Line Shapes ◽  
Acyl Chains ◽  
Nuclear Magnetic

The binding of the local anesthetics tetracaine and procaine with multilamellar dispersions of egg phosphatidylcholine has been studied by 2H nuclear magnetic resonance (NMR). The 2H-NMR line shapes of specifically deuterated local anesthetics are found to be very dependent on the attainment of a true equilibrium. The equilibrium could be most properly reached by the use of repeated freeze–thaw–vortex cycles. The data for tetracaine are consistent with the three-site exchange model proposed earlier. Tetracaine is in slow exchange between a strongly bound site and a weakly bound site and in fast exchange between the weakly bound site and free in solution. The slow exchange rate is estimated, from temperature and dilution studies, to be approximately 1.5 × 103 s−1 at pH 5.5 and slightly faster at pH 9.5. Comparisons of the quadrupole splittings with those seen for our earlier work in egg phosphatidylethanolamine suggest that the location of the strongly bound site in phosphatidylcholine is dependent on the anesthetic charge. This is in contrast to egg phosphatidylethanolamine, where molecular shapes appear to be the determining factor for the location of the anesthetic. Procaine bound very weakly to the model membranes, to yield only a broad resonance and no quadrupole splitting. It appears that procaine, unlike tetracaine, is not bound by the ordered acyl chains.

Sign in / Sign up

Export Citation Format

Share Document