scholarly journals A multiplexed immunoassay system based upon reciprocating centrifugal microfluidics

2011 ◽  
Vol 82 (6) ◽  
pp. 064303 ◽  
Author(s):  
Zahra Noroozi ◽  
Horacio Kido ◽  
Régis Peytavi ◽  
Rie Nakajima-Sasaki ◽  
Algimantas Jasinskas ◽  
...  
Keyword(s):  
Centrifugal Microfluidics ◽  
Multiplexed Immunoassay

scholarly journals Use of a multiplexed immunoassay for detection of serotype-specific Streptococcus pneumoniae antigen in pleural fluid and cerebrospinal fluid specimens

2011 ◽  
Vol 60 (12) ◽  
pp. 1879-1881 ◽  
Author(s):  
Carmen L. Sheppard ◽  
Malcolm Guiver ◽  
John Hartley ◽  
Timothy G. Harrison ◽  
Robert C. George
Keyword(s):  
Cerebrospinal Fluid ◽  
Streptococcus Pneumoniae ◽  
Pleural Fluid ◽  
Multiplexed Immunoassay

Sampling-Resolution Strategy for One-Way Multiplexed Immunoassay with Sequential Chemiluminescent Detection

2008 ◽  
Vol 80 (14) ◽  
pp. 5654-5659 ◽  
Author(s):  
Hong Liu ◽  
Zhifeng Fu ◽  
Zhanjun Yang ◽  
Feng Yan ◽  
Huangxian Ju
Keyword(s):  
Chemiluminescent Detection ◽  
Resolution Strategy ◽  
Multiplexed Immunoassay

Development of All-Plastic Microvalve Array for Multiplexed Immunoassay

2014 ◽  
Author(s):  
Shancy Augustine ◽  
Pan Gu ◽  
Xiangjun Zheng ◽  
Toshikazu Nishida ◽  
Z. Hugh Fan
Keyword(s):  
Large Scale ◽  
Low Cost ◽  
Simultaneous Detection ◽  
Cost Effective ◽  
Negative Control ◽  
Sensitive Material ◽  
Detection Scheme ◽  
Power Battery ◽  
Multiple Analytes ◽  
Multiplexed Immunoassay

There is a need for low-cost immunoassays that measure the presence and concentration of multiple harmful agents in one device. Currently, comparable immunoassays employ a one-analyte-per-test format that is time consuming and not cost effective for the requirement of detecting multiple analytes in a single sample. For instance, if a spectrum of harmful agents, including E. coli O157, cholera toxin, and Salmonella typhimurium, should be simultaneously monitored in foods and drinking water, then a one-analyte-per-test would be inefficient. This work demonstrates a platform capable of simultaneous detection of multiple analytes in a single, low-cost, microvalve array-enabled multiplexed immunoassay. This multiplexed immunoassay platform is demonstrated in a prototype COC (cyclic olefin copolymer) device with a 2×3 array in which 6 analytes can be detected simultaneously. In order to contain and regulate the flow of reagents in the multichannel device, an array of microfluidic valves actuated by a thermally expandable material and microfabricated resistors have been developed to direct the flow to the necessary assay sites. The microvalve-based immunoassay is shown to be reliable, easy to operate, and compatible with large-scale integration. The all-plastic microvalves use paraffin wax as the thermally sensitive material which drastically reduces power consumption by latching upon closing so that pulsed power is required only to close and latch the microvalve until it is necessary to re-open the valve. The multiplexed detection scheme has been demonstrated by using three proteins, C reactive protein (CRP) and transferrin, both of which are biomarkers associated with traumatic brain injury (TBI) as well as bovine serum albumin (BSA) as the negative control. Since there are no external bulky pneumatic accessories required to operate/latch the microvalves in the device, this compact, thermally actuated and latching microvalve-enabled multiplexed immunoassay has the potential to realize a portable, low power, battery operated microfluidic device for biological assays.

Multiplexed immunoassay of thyroglobulin autoantibodies in patients with differentiated thyroid carcinoma

Head & Neck ◽  
2011 ◽  
Vol 34 (10) ◽  
pp. 1369-1371 ◽  
Author(s):  
Luca Giovanella ◽  
Emanuela Toffalori ◽  
Renato Tozzoli ◽  
Marco Caputo ◽  
Luca Ceriani ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Differentiated Thyroid Carcinoma ◽  
Thyroglobulin Autoantibodies ◽  
Multiplexed Immunoassay

scholarly journals The eLoaD platform endows centrifugal microfluidics with on-disc power and communication

2018 ◽  
Vol 117 ◽  
pp. 464-473 ◽  
Author(s):  
Saraí M. Torres Delgado ◽  
Jan G. Korvink ◽  
Dario Mager
Keyword(s):  
Centrifugal Microfluidics

Polymerized cholesteric liquid crystal microdisks generated by centrifugal microfluidics towards tunable laser emissions [Invited]

2020 ◽  
Vol 18 (8) ◽  
pp. 080006
Author(s):  
Ya-Hao Ge ◽  
Yi-Mei Lan ◽  
Xing-Rui Li ◽  
Yu-Wei Shan ◽  
Yu-Jie Yang ◽  
...  
Keyword(s):  
Liquid Crystal ◽  
Cholesteric Liquid Crystal ◽  
Tunable Laser ◽  
Centrifugal Microfluidics

Multiplexed immunoassay using post-synthesis functionalized hydrogel microparticles

Lab on a Chip ◽  
2019 ◽  
Vol 19 (1) ◽  
pp. 111-119 ◽  
Author(s):  
Hyun Jee Lee ◽  
Yoon Ho Roh ◽  
Hyeon Ung Kim ◽  
Sun Min Kim ◽  
Ki Wan Bong
Keyword(s):  
High Sensitivity ◽  
Fast Detection ◽  
Hydrogel Microparticles ◽  
Post Synthesis ◽  
Multiplexed Immunoassay

Post-synthesis functionalized hydrogel microparticles were demonstrated in multiplex immunoassays with high sensitivity, a wide assay range, and fast detection.

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