Multiplexed immunoassay using post-synthesis functionalized hydrogel microparticles

Lab on a Chip ◽  
2019 ◽  
Vol 19 (1) ◽  
pp. 111-119 ◽  
Author(s):  
Hyun Jee Lee ◽  
Yoon Ho Roh ◽  
Hyeon Ung Kim ◽  
Sun Min Kim ◽  
Ki Wan Bong
Keyword(s):  
High Sensitivity ◽  
Fast Detection ◽  
Hydrogel Microparticles ◽  
Post Synthesis ◽  
Multiplexed Immunoassay

Post-synthesis functionalized hydrogel microparticles were demonstrated in multiplex immunoassays with high sensitivity, a wide assay range, and fast detection.

scholarly journals Nanopore Technology for the Application of Protein Detection

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1942
Author(s):  
Xiaoqing Zeng ◽  
Yang Xiang ◽  
Qianshan Liu ◽  
Liang Wang ◽  
Qianyun Ma ◽  
...  
Keyword(s):  
Single Molecule ◽  
Protein Detection ◽  
High Sensitivity ◽  
Single Molecule Detection ◽  
Sequence Detection ◽  
Fast Detection ◽  
Material Basis ◽  
Sensing Technology ◽  
Detection Technology ◽  
Structure Recognition

Protein is an important component of all the cells and tissues of the human body and is the material basis of life. Its content, sequence, and spatial structure have a great impact on proteomics and human biology. It can reflect the important information of normal or pathophysiological processes and promote the development of new diagnoses and treatment methods. However, the current techniques of proteomics for protein analysis are limited by chemical modifications, large sample sizes, or cumbersome operations. Solving this problem requires overcoming huge challenges. Nanopore single molecule detection technology overcomes this shortcoming. As a new sensing technology, it has the advantages of no labeling, high sensitivity, fast detection speed, real-time monitoring, and simple operation. It is widely used in gene sequencing, detection of peptides and proteins, markers and microorganisms, and other biomolecules and metal ions. Therefore, based on the advantages of novel nanopore single-molecule detection technology, its application to protein sequence detection and structure recognition has also been proposed and developed. In this paper, the application of nanopore single-molecule detection technology in protein detection in recent years is reviewed, and its development prospect is investigated.

scholarly journals Optimization of Velocity Flap Structures in High Sensitivity Macrofluidic Airflow Sensor

2018 ◽  
Vol 7 (4.27) ◽  
pp. 11
Author(s):  
Mohamad Dzulhelmy bin Amari ◽  
Muhamad Saifuddin b. Abdull Shukor ◽  
Sukarnur Che Abdullah
Keyword(s):  
High Sensitivity ◽  
Low Velocity Impact ◽  
Low Velocity ◽  
Velocity Impact ◽  
Fast Detection ◽  
Intelligent Control System ◽  
Highly Sensitive ◽  
Computational Fluid Dynamics Cfd ◽  
Airflow Sensor ◽  
Program Software

Automated reaction from the system is most important in fulfilling the requirement of the intelligent control system. Hence, many related studies regarding in developing the hardware of the system such as high sensitivity of the airflow sensor in detecting the changes either in user or the environment. The effect of the fast detection of the sensor through the high sensitivity of the airflow sensor have enable the system to identify and analyze the behavior of the user in higher accuracy compared to conventional system. Within the scope of airflow sensitivity, separation between two parts in the airflow sensor in altering the velocity impact have been inquired in purpose, while a few investigations in relations to determine the pressure contour of the main parts have been explored by application of using Computational Fluid Dynamics (CFD. This simulation is performed in the ANSYS program software. Thus, this study consequently intends to be focus on detection the high sensitivity of the airflow movement by distinguishing the high and low velocity impact. The optimization the airflow sensor in this study based on design parameter also done in order to design and develop a highly sensitive airflow sensor   

scholarly journals Application of Electrochemical Sensors Based on Carbon Nanomaterials for Detection of Flavonoids

Nanomaterials ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 2020
Author(s):  
Jinchun Hu ◽  
Zhenguo Zhang
Keyword(s):  
Carbon Nanomaterials ◽  
Electrochemical Sensors ◽  
Graphene Quantum Dots ◽  
High Sensitivity ◽  
Research Field ◽  
Electrochemical Sensing ◽  
Detection Methods ◽  
Health Issues ◽  
Fast Detection ◽  
Anti Cancer

Flavonoids have a variety of physiological activities such as anti-free radicals, regulating hormone levels, antibacterial factors, and anti-cancer factors, which are widely present in edible and medicinal plants. Real-time detection of flavonoids is a key step in the quality control of diverse matrices closely related to social, economic, and health issues. Traditional detection methods are time-consuming and require expensive equipment and complicated working conditions. Therefore, electrochemical sensors with high sensitivity and fast detection speed have aroused extensive research interest. Carbon nanomaterials are preferred material in improving the performance of electrochemical sensing. In this paper, we review the progress of electrochemical sensors based on carbon nanomaterials including carbon nanotubes, graphene, carbon and graphene quantum dots, mesoporous carbon, and carbon black for detecting flavonoids in food and drug homologous substances in the last four years. In addition, we look forward to the prospects and challenges of this research field.

DEVELOPMENT OF MATERIALS AND SAMPLING METHODS FOR IR-BASED DETECTION OF TOXIC COMPOUNDS IN WATER

2007 ◽  
Vol 17 (04) ◽  
pp. 697-708
Author(s):  
BRIAN J. NINNESS ◽  
LUKE D. DOUCETTE ◽  
BEN MCCOOL ◽  
CARL P. TRIPP
Keyword(s):  
High Sensitivity ◽  
False Alarms ◽  
Material Synthesis ◽  
Sampling Protocol ◽  
Toxic Compounds ◽  
Fast Detection ◽  
Oxide Powders ◽  
Detection Approach ◽  
Aqueous Environments

A detection approach based on the principles of Fourier Transform Infrared Spectroscopy (FTIR) is presented for the trace level detection of toxic compounds in water. The main advantages of this approach are that it operates in heterogeneous aqueous environments, provides fast detection (< 10 min), and exhibits high sensitivity/selectivity to nonvolatile toxic materials with minimal false alarms. The key enablers to using FTIR for aqueous-based detection is the development of a selective and robust sampling protocol coupled to a miniaturized portable FTIR unit. The sampling approaches involve synthesizing and tailoring microporous, mesoporous, and nonporous metal oxide powders/films that are amenable for in situ FTIR measurements. In this paper we provide an overview of the material synthesis and surface modification strategies, and present results obtained using these materials for the low level detection of the organophosphate pesticide phosmet. Phosmet is used as a surrogate for the nerve agent VX.

Post-synthesis functionalized hydrogel microparticles for high performance microRNA detection

2019 ◽  
Vol 1076 ◽  
pp. 110-117 ◽  
Author(s):  
Yoon Ho Roh ◽  
Hyun Jee Lee ◽  
Hyun June Moon ◽  
Sun Min Kim ◽  
Ki Wan Bong
Keyword(s):  
High Performance ◽  
Hydrogel Microparticles ◽  
Microrna Detection ◽  
Post Synthesis

scholarly journals Verification Test of High Flap Macrofluidic Air Flow Sensor in Wind Tunnel

2019 ◽  
Vol 9 (1) ◽  
pp. 5672-5676
Keyword(s):  
Wind Tunnel ◽  
Air Flow ◽  
High Sensitivity ◽  
Flow Sensor ◽  
Fast Detection ◽  
Degree Of Orientation ◽  
Verification Test ◽  
Airflow Sensor ◽  
Space Interval

This air sensor functioning to detect the speed of air surrounding while in motionor a sudden changes in its environment. The effect of fast detection of a security sensor through the high sensitivity of the airflow sensor has enabled the system to identify and analyze the critical condition in higher accuracy compared to the conventional of any security system. Previous studies have developed the macrofluidic air flow sensor that observed the air flow in higher accuracy while the sensor in motion will be verified by detection of high sensitivity in the relative velocity of the airflow sensor compared to a conventional sensor. An experimental investigation was conducted to verify macrofluidic air flow sensor in wind tunnel by control velocity of range (30 to 110 km/h). The result shows the characterization of the changes in voltage reading with respect to the airflow speed in the wind tunnel. Sensor 1 to 4 have been placed at 0 to 360 degree of orientation with respective of 90 degree space interval.

scholarly journals The Application of Nucleic Acid Probe–Based Fluorescent Sensing and Imaging in Cancer Diagnosis and Therapy

2021 ◽  
Vol 9 ◽  
Author(s):  
Ge Huang ◽  
Chen Su ◽  
Lijuan Wang ◽  
Yanxia Fei ◽  
Jinfeng Yang
Keyword(s):  
Nucleic Acid ◽  
Cancer Diagnosis ◽  
High Sensitivity ◽  
Good Tolerance ◽  
Fast Detection ◽  
Fluorescent Sensing ◽  
Cancer Management ◽  
Management Techniques ◽  
Cancer Theranostics ◽  
Cancer Diagnosis And Therapy

It is well known that cancer incidence and death rates have been growing, but the development of cancer theranostics and therapeutics has been a challenging work. Recently, nucleic acid probe–based fluorescent sensing and imaging have achieved remarkable improvements in a variety of cancer management techniques, credited to their high sensitivity, good tolerance to interference, fast detection, and high versatility. Herein, nucleic acid probe–based fluorescent sensing and imaging are labeled with advanced fluorophores, which are essential for fast and sensitive detection of aberrant nucleic acids and other cancer-relevant molecules, consequently performing cancer early diagnosis and targeted treatment. In this review, we introduce the characteristics of nucleic acid probes, summarize the development of nucleic acid probe–based fluorescent sensing and imaging, and prominently elaborate their applications in cancer diagnosis and treatment. In discussion, some challenges and perspectives are elaborated in the field of nucleic acid probe–based fluorescent sensing and imaging.

scholarly journals A Review on Functionalized Graphene Sensors for Detection of Ammonia

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1443
Author(s):  
Xiaohui Tang ◽  
Marc Debliquy ◽  
Driss Lahem ◽  
Yiyi Yan ◽  
Jean-Pierre Raskin
Keyword(s):  
Metal Oxides ◽  
Gas Sensors ◽  
Mass Production ◽  
Metallic Nanoparticles ◽  
Room Temperature ◽  
Organic Molecules ◽  
High Sensitivity ◽  
Functionalized Graphene ◽  
Fast Detection ◽  
Sensor Mass

Since the first graphene gas sensor has been reported, functionalized graphene gas sensors have already attracted a lot of research interest due to their potential for high sensitivity, great selectivity, and fast detection of various gases. In this paper, we summarize the recent development and progression of functionalized graphene sensors for ammonia (NH3) detection at room temperature. We review graphene gas sensors functionalized by different materials, including metallic nanoparticles, metal oxides, organic molecules, and conducting polymers. The various sensing mechanism of functionalized graphene gas sensors are explained and compared. Meanwhile, some existing challenges that may hinder the sensor mass production are discussed and several related solutions are proposed. Possible opportunities and perspective applications of the graphene NH3 sensors are also presented.

REAGENTLESS BIO-SAMPLING METHODS FOR IR DETECTION

2007 ◽  
Vol 17 (04) ◽  
pp. 729-737 ◽  
Author(s):  
LUKE D. DOUCETTE ◽  
HE LI ◽  
BRIAN J. NINNESS ◽  
CARL P. TRIPP
Keyword(s):  
Detection System ◽  
High Sensitivity ◽  
Attenuated Total Reflection ◽  
False Alarms ◽  
Flow Cells ◽  
Fast Detection ◽  
Detection Systems ◽  
Sampling Protocols ◽  
Ir Detection ◽  
The Military

Currently there exists a critical need within the military and homeland defense for highly sophisticated yet, small, lightweight portable sensors and detection systems for identifying and quantifying biological and biowarfare agents (BWA) in both liquid and aerosolized form. Our proposed BWA detection system is based upon Fourier Transform Infrared Spectroscopy (FTIR), where the main advantages of this approach are that it is reagentless, operates in heterogeneous aqueous environments, and provides fast detection and high sensitivity/selectivity to bacterial spores with minimal false alarms. The key enabler to using FTIR for BWA detection is to develop selective and robust sampling protocols coupled to a miniaturized, portable FTIR unit. To that end, we have developed front-end liquid flow cells which incorporate electric field (E-Field) concentration methods for spores onto the surface of an Attenuated Total Reflection (ATR) IR crystal. IR spectra are presented which show collection and detection results with BG spores in water. The approaches we have developed take advantage of the fact that all spores are negatively charged in neutral pH solutions. Therefore, E-Field concentration of spores directly onto an ATR sampling element enables low level concentration measurements to be possible.

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