Design and operation of a portable scanner for high performance microchip capillary array electrophoresis

2010 ◽  
Vol 81 (11) ◽  
pp. 113105 ◽  
Author(s):  
James R. Scherer ◽  
Peng Liu ◽  
Richard A. Mathies
Keyword(s):  
High Performance ◽  
Capillary Array Electrophoresis ◽  
Capillary Array

scholarly journals Genotyping Energy-Transfer-Cassette-labeled Short-Tandem-Repeat Amplicons with Capillary Array Electrophoresis Microchannel Plates

2001 ◽  
Vol 47 (9) ◽  
pp. 1614-1621 ◽  
Author(s):  
Igor L Medintz ◽  
Lorenzo Berti ◽  
Charles A Emrich ◽  
Jennifer Tom ◽  
James R Scherer ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Genetic Analysis ◽  
High Throughput ◽  
High Performance ◽  
Microchannel Plate ◽  
Str Analysis ◽  
Capillary Array Electrophoresis ◽  
Microchannel Plates ◽  
Capillary Array ◽  
Short Tandem

Abstract Background: Genetic analysis of microsatellite DNA is a powerful tool used in linkage analysis, gene mapping, and clinical diagnosis. To address the expanding needs of studies of short tandem repeats (STRs), we demonstrated high-performance STR analysis on a high-throughput microchannel plate-based platform. Methods: Energy-transfer-cassette-labeled STR amplicons were separated and typed on a microfabricated 96-channel radial capillary array electrophoresis (CAE) microchannel plate system. Four-color detection was accomplished with a laser-excited confocal fluorescence rotary scanner. Results: Multiplex STR analysis with single base-pair resolution was demonstrated on denaturing polyacrylamide gel media. The high-throughput multiplex capabilities of this genetic analysis platform were demonstrated by the simultaneous separation of STR amplicons representing 122 samples in ninety-six 5.5-cm-long channels in <8 min. Sizing values obtained for these amplicons on the CAE microchannel plate were comparable to those measured on a conventional commercial CAE instrument and exhibit <1% sizing variance. Conclusions: Energy-transfer-cassette labeling and microfabricated CAE microchannel plates allow high-performance multiplex STR analyses.

High-Performance Genetic Analysis on Microfabricated Capillary Array Electrophoresis Plastic Chips Fabricated by Injection Molding

2005 ◽  
Vol 77 (7) ◽  
pp. 2140-2146 ◽  
Author(s):  
Fuquan Dang ◽  
Osamu Tabata ◽  
Masaya Kurokawa ◽  
Ashraf A. Ewis ◽  
Lihua Zhang ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Injection Molding ◽  
High Performance ◽  
Capillary Array Electrophoresis ◽  
Capillary Array

High-performance genetic analysis using microfabricated capillary array electrophoresis microplates

2001 ◽  
Vol 22 (18) ◽  
pp. 3845-3856 ◽  
Author(s):  
Igor L. Medintz ◽  
Brian M. Paegel ◽  
Robert G. Blazej ◽  
Charles A. Emrich ◽  
Lorenzo Berti ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
High Performance ◽  
Capillary Array Electrophoresis ◽  
Capillary Array

Radial Capillary Array Electrophoresis Microplate and Scanner for High-Performance Nucleic Acid Analysis

1999 ◽  
Vol 71 (23) ◽  
pp. 5354-5361 ◽  
Author(s):  
Yining Shi ◽  
Peter C. Simpson ◽  
James R. Scherer ◽  
David Wexler ◽  
Christine Skibola ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Performance ◽  
Capillary Array Electrophoresis ◽  
Nucleic Acid Analysis ◽  
Capillary Array

MICRO CAPILLARY ARRAY ELECTROPHORESIS CHIP BY MOVING MASK LIGA TECHNOLOGY

2004 ◽  
Vol 01 (01) ◽  
pp. 39-46
Author(s):  
HUI YOU ◽  
SHOUJI SHINOHARA ◽  
KENNICHI ENAMI ◽  
SHINSUKE SHIBATA ◽  
OSAMU TABATA ◽  
...  
Keyword(s):  
Adhesive Bonding ◽  
Dna Analysis ◽  
Adhesive Layer ◽  
Fabrication Process ◽  
Optical Components ◽  
X Ray ◽  
Laser Induced Fluorescence Detection ◽  
Capillary Array Electrophoresis ◽  
Liga Technology ◽  
Capillary Array

The concept and the fabrication process of a micro capillary array electrophoresis (μ-CAE) for DNA analysis were stated. The chip was mainly made of plastic and fabricated by the innovatory LIGA technology which included moving mask deep X-ray lithography, electroplating and hot embossing, and assembled by an adhesive bonding, in which the adhesive layer solidified and formed firm bond under UV exposure. The micro channel array with high aspect ratio is the key part, whose wall should have a slight inclination to ensure the demoulding. It was demonstrated that moving mask deep X-ray lithography (M2DXL) technology could successfully control the inclination and enabled to integrate micro optical components such as micro lens into the chip, which improved detection performance greatly. The fabrication process was experimented and some initial prototype chips have been obtained. Fluidic test and electrophoresis test with laser-induced fluorescence detection has been done on the prototype. The results confirm that the μ-CAE by moving mask LIGA process is realizable and it has great potential in high-throughput, which may lead to ultra-fast DNA analysis.

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