Radial Capillary Array Electrophoresis Microplate and Scanner for High-Performance Nucleic Acid Analysis

1999 ◽  
Vol 71 (23) ◽  
pp. 5354-5361 ◽  
Author(s):  
Yining Shi ◽  
Peter C. Simpson ◽  
James R. Scherer ◽  
David Wexler ◽  
Christine Skibola ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Performance ◽  
Capillary Array Electrophoresis ◽  
Nucleic Acid Analysis ◽  
Capillary Array

scholarly journals Genotyping Energy-Transfer-Cassette-labeled Short-Tandem-Repeat Amplicons with Capillary Array Electrophoresis Microchannel Plates

2001 ◽  
Vol 47 (9) ◽  
pp. 1614-1621 ◽  
Author(s):  
Igor L Medintz ◽  
Lorenzo Berti ◽  
Charles A Emrich ◽  
Jennifer Tom ◽  
James R Scherer ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Genetic Analysis ◽  
High Throughput ◽  
High Performance ◽  
Microchannel Plate ◽  
Str Analysis ◽  
Capillary Array Electrophoresis ◽  
Microchannel Plates ◽  
Capillary Array ◽  
Short Tandem

Abstract Background: Genetic analysis of microsatellite DNA is a powerful tool used in linkage analysis, gene mapping, and clinical diagnosis. To address the expanding needs of studies of short tandem repeats (STRs), we demonstrated high-performance STR analysis on a high-throughput microchannel plate-based platform. Methods: Energy-transfer-cassette-labeled STR amplicons were separated and typed on a microfabricated 96-channel radial capillary array electrophoresis (CAE) microchannel plate system. Four-color detection was accomplished with a laser-excited confocal fluorescence rotary scanner. Results: Multiplex STR analysis with single base-pair resolution was demonstrated on denaturing polyacrylamide gel media. The high-throughput multiplex capabilities of this genetic analysis platform were demonstrated by the simultaneous separation of STR amplicons representing 122 samples in ninety-six 5.5-cm-long channels in <8 min. Sizing values obtained for these amplicons on the CAE microchannel plate were comparable to those measured on a conventional commercial CAE instrument and exhibit <1% sizing variance. Conclusions: Energy-transfer-cassette labeling and microfabricated CAE microchannel plates allow high-performance multiplex STR analyses.

High-Performance Genetic Analysis on Microfabricated Capillary Array Electrophoresis Plastic Chips Fabricated by Injection Molding

2005 ◽  
Vol 77 (7) ◽  
pp. 2140-2146 ◽  
Author(s):  
Fuquan Dang ◽  
Osamu Tabata ◽  
Masaya Kurokawa ◽  
Ashraf A. Ewis ◽  
Lihua Zhang ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Injection Molding ◽  
High Performance ◽  
Capillary Array Electrophoresis ◽  
Capillary Array

High-performance genetic analysis using microfabricated capillary array electrophoresis microplates

2001 ◽  
Vol 22 (18) ◽  
pp. 3845-3856 ◽  
Author(s):  
Igor L. Medintz ◽  
Brian M. Paegel ◽  
Robert G. Blazej ◽  
Charles A. Emrich ◽  
Lorenzo Berti ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
High Performance ◽  
Capillary Array Electrophoresis ◽  
Capillary Array
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