scholarly journals Amplification of SPPS150 andSalmonella typhiDNA with a high throughput oscillating flow polymerase chain reaction device

2010 ◽  
Vol 4 (2) ◽  
pp. 024103 ◽  
Author(s):  
D. Sugumar ◽  
Asma Ismail ◽  
Manickam Ravichandran ◽  
Ismail Aziah ◽  
L. X. Kong
Keyword(s):  
Polymerase Chain Reaction ◽  
High Throughput ◽  
Oscillating Flow ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Reaction Device

scholarly journals Sustained high-throughput polymerase chain reaction diagnostics during the European epidemic of Bluetongue virus serotype 8

2012 ◽  
Vol 24 (3) ◽  
pp. 469-478 ◽  
Author(s):  
Piet A. van Rijn ◽  
René G. Heutink ◽  
Jan Boonstra ◽  
Hans A. Kramps ◽  
René G. P. van Gennip
Keyword(s):  
Polymerase Chain Reaction ◽  
High Throughput ◽  
Bluetongue Virus ◽  
Chain Reaction ◽  
Virus Serotype ◽  
Polymerase Chain

Quantum dots for a high-throughput Pfu polymerase based multi-round polymerase chain reaction (PCR)

The Analyst ◽  
2018 ◽  
Vol 143 (5) ◽  
pp. 1259-1267 ◽  
Author(s):  
Fuming Sang ◽  
Zhizhou Zhang ◽  
Lin Yuan ◽  
Deli Liu
Keyword(s):  
Polymerase Chain Reaction ◽  
Quantum Dots ◽  
High Throughput ◽  
Chain Reaction ◽  
Round Polymerase Chain Reaction ◽  
Polymerase Chain

We developed a Pfu polymerase based multi-round PCR technique assisted by quantum dots (QDs).

scholarly journals Evaluation of a 384–Well Format for High-Throughput Real-Time Reverse Transcription Polymerase Chain Reaction for Avian Influenza Testing

2009 ◽  
Vol 21 (5) ◽  
pp. 679-683 ◽  
Author(s):  
Pamela J. Ferro ◽  
Jason Osterstock ◽  
Bo Norby ◽  
Geoffrey T. Fosgate ◽  
Blanca Lupiani
Keyword(s):  
Polymerase Chain Reaction ◽  
Avian Influenza ◽  
Real Time ◽  
High Throughput ◽  
Reverse Transcription ◽  
Virus Isolation ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Response Planning ◽  
Polymerase Chain

As concerns over the global spread of highly pathogenic avian influenza H5N1 have heightened, more countries are faced with increased surveillance efforts and incident response planning for handling a potential outbreak. The incorporation of molecular techniques in most diagnostic laboratories has enabled fast and efficient testing of many agents of concern, including avian influenza. However, the need for high-throughput testing remains. In this study, the use of a 384–well format for high-throughput real-time reverse transcription polymerase chain reaction (real-time RT-PCR) testing for avian influenza is described. The analytical sensitivity of a real-time RT-PCR assay for avian influenza virus matrix gene with the use of both 96– and 384–well assay formats and serial dilutions of transcribed control RNA were comparable, resulting in similar limits of detection. Of 28 hunter-collected cloacal swabs that were positive by virus isolation, 26 (92.9%) and 27 (96.4%) were positive in the 96– and 384–well assays, respectively; of the 340 hunter-collected swabs that were negative by virus isolation, 45 (13.2%) and 23 (6.8%) were positive in the 96– and 384–well assays, respectively. The data presented herein supports the utility of the 384–well format in the event of an avian influenza outbreak for high-throughput real-time RT-PCR testing.

Multidisciplinary Design and Optimization for Oscillating Flow Polymerase Chain Reaction Microfluidics Device

2009 ◽  
Author(s):  
Tohru Suwa ◽  
Hamid Hadim ◽  
Yong Shi
Keyword(s):  
Polymerase Chain Reaction ◽  
Contact Area ◽  
Channel Wall ◽  
Thermal Response ◽  
Oscillating Flow ◽  
Temperature Zone ◽  
Chain Reaction ◽  
Pdms Surface ◽  
Design And Optimization ◽  
Polymerase Chain

A Polymerase Chain Reaction (PCR) process is almost always required prior to DNA (deoxyribonucleic acid) analysis to create multiple copies of DNA fragments. Using microfluidics technology, the PCR process requires much shorter process time and much less DNA samples than conventional PCR systems. Among existing microfluidics-based techniques, the oscillating flow PCR has advantages including faster analysis time than cavity PCR microfluidics, and smaller contact area between the sample and polymer channel wall compared to flow-through PCR. The smaller contact area reduces DNA adsorption and enhances DNA detection accuracy. In the proposed study, new design features of the oscillating flow PCR concept are evaluated including: (1) PDMS (polydimethylsiloxane) and glass are selected as the microfluidics chip material for realizing a disposable chip, (2) water impingement cooling is applied to effectively isolate the temperature zones, and (3) a copper layer is attached outside of the chip to enhance uniform temperature distribution within the temperature zones. When PDMS is used for PCR microfluidics devices, lower efficiency has been a disadvantage. The efficiency is lowered because the DNA fragments are trapped at the PDMS surface. This trapping can be reduced by minimizing the contact area between the sample and the PDMS surface. When the sample contact area is reduced, which can be achieved by increasing the flow channel cross-sectional area, thermal response is degraded. Optimal channel dimensions are determined by considering the trade-off between thermal response and sample contact area with PDMS channel wall. The resulting thermal response of the sample in the temperature zone is comparable to existing studies, which use silicon as the chip material. A transient FEM heat transfer analysis for the temperature zone is performed for more effective thermal design and optimization.

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