Synthesis and intrinsic optical properties of retinal Schiff base and Green Fluorescent Protein chromophores

2008 ◽  
Author(s):  
Mogens Brøndsted Nielsen ◽  
Michael Axman Petersen ◽  
Lars H. Andersen ◽  
V. K. Vaidyan ◽  
V. S. Jayakumar
Keyword(s):  
Optical Properties ◽  
Schiff Base ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Green Fluorescent

scholarly journals Nonlinear Optical Properties of Green Fluorescent Protein Chromophore Coupled Diradicals

2013 ◽  
Vol 29 (12) ◽  
pp. 2543-2550
Author(s):  
YU Hai-Ling ◽  
◽  
ZHANG Meng-Ying ◽  
HONG Bo ◽  
CHENG Zhi Qiang ◽  
...  
Keyword(s):  
Optical Properties ◽  
Green Fluorescent Protein ◽  
Nonlinear Optical ◽  
Fluorescent Protein ◽  
Nonlinear Optical Properties ◽  
Green Fluorescent

Expression and optical properties of green fluorescent protein expressed in different cellular environments

2005 ◽  
Vol 119 (4) ◽  
pp. 368-378 ◽  
Author(s):  
Jin Zou ◽  
Yiming Ye ◽  
Kristy Welshhans ◽  
Monica Lurtz ◽  
April L. Ellis ◽  
...  
Keyword(s):  
Optical Properties ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Green Fluorescent

scholarly journals A Green Fluorescent Protein Containing a QFG Tri-Peptide Chromophore: Optical Properties and X-Ray Crystal Structure

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47331 ◽  
Author(s):  
Jion M. Battad ◽  
Daouda A. K. Traore ◽  
Emma Byres ◽  
Jamie Rossjohn ◽  
Rodney J. Devenish ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Optical Properties ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
X Ray ◽  
Green Fluorescent

scholarly journals Cathodoluminescence of green fluorescent protein exhibits the redshifted spectrum and the robustness

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Keiichirou Akiba ◽  
Katsuyuki Tamehiro ◽  
Koki Matsui ◽  
Hayata Ikegami ◽  
Hiroki Minoda
Keyword(s):  
Optical Properties ◽  
Electron Beam ◽  
Electron Microscope ◽  
Green Fluorescent Protein ◽  
Electron Beam Irradiation ◽  
Fluorescent Protein ◽  
Structural Information ◽  
Ion Beam ◽  
Beam Irradiation ◽  
Green Fluorescent

Abstract Green fluorescent protein (GFP) and its variants are an essential tool for visualizing functional units in biomaterials. This is achieved by the fascinating optical properties of them. Here, we report novel optical properties of enhanced GFP (EGFP), which is one of widely used engineered variants of the wild-type GFP. We study the electron-beam-induced luminescence, which is known as cathodoluminescence (CL), using the hybrid light and transmission electron microscope. Surprisingly, even from the same specimen, we observe a completely different dependences of the fluorescence and CL on the electron beam irradiation. Since light emission is normally independent of whether an electron is excited to the upper level by light or by electron beam, this difference is quite peculiar. We conclude that the electron beam irradiation causes the local generation of a new redshifted form of EGFP and CL is preferentially emitted from it. In addition, we also find that the redshifted form is rather robust to electron bombardment. These remarkable properties can be utilized for three-dimensional reconstruction without electron staining in focused ion beam/scanning electron microscopy technology and provide significant potential for simultaneously observing the functional information specified by super-resolution CL imaging and the structural information at the molecular level obtained by electron microscope.

Sign in / Sign up

Export Citation Format

Share Document