Studies of pH-Sensitive Optical Properties of the deGFP1 Green Fluorescent Protein Using a Unique Polarizable Force Field

2014 ◽  
Vol 10 (8) ◽  
pp. 3492-3502 ◽  
Author(s):  
I. Harczuk ◽  
N. Arul Murugan ◽  
O. Vahtras ◽  
H. Ågren
Keyword(s):  
Optical Properties ◽  
Green Fluorescent Protein ◽  
Force Field ◽  
Fluorescent Protein ◽  
Ph Sensitive ◽  
Polarizable Force Field ◽  
Green Fluorescent

scholarly journals Nonlinear Optical Properties of Green Fluorescent Protein Chromophore Coupled Diradicals

2013 ◽  
Vol 29 (12) ◽  
pp. 2543-2550
Author(s):  
YU Hai-Ling ◽  
◽  
ZHANG Meng-Ying ◽  
HONG Bo ◽  
CHENG Zhi Qiang ◽  
...  
Keyword(s):  
Optical Properties ◽  
Green Fluorescent Protein ◽  
Nonlinear Optical ◽  
Fluorescent Protein ◽  
Nonlinear Optical Properties ◽  
Green Fluorescent

Cleavage of progastrin-derived peptides is modulated by intravesicular PH: Studies using PH-sensitive green fluorescent protein directed to secretory vesicles

2000 ◽  
Vol 118 (4) ◽  
pp. A712
Author(s):  
Colin Blackmore ◽  
Lisa Bishop ◽  
Andrea Varro ◽  
Rod Dimaline ◽  
David V. Gallacher ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Ph Sensitive ◽  
Secretory Vesicles ◽  
Green Fluorescent

scholarly journals M153R Mutation in a pH-Sensitive Green Fluorescent Protein Stabilizes Its Fusion Proteins

PLoS ONE ◽  
2011 ◽  
Vol 6 (5) ◽  
pp. e19598 ◽  
Author(s):  
Yusuke V. Morimoto ◽  
Seiji Kojima ◽  
Keiichi Namba ◽  
Tohru Minamino
Keyword(s):  
Green Fluorescent Protein ◽  
Fusion Proteins ◽  
Fluorescent Protein ◽  
Ph Sensitive ◽  
Green Fluorescent

Expression and optical properties of green fluorescent protein expressed in different cellular environments

2005 ◽  
Vol 119 (4) ◽  
pp. 368-378 ◽  
Author(s):  
Jin Zou ◽  
Yiming Ye ◽  
Kristy Welshhans ◽  
Monica Lurtz ◽  
April L. Ellis ◽  
...  
Keyword(s):  
Optical Properties ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Green Fluorescent

scholarly journals A Green Fluorescent Protein Containing a QFG Tri-Peptide Chromophore: Optical Properties and X-Ray Crystal Structure

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47331 ◽  
Author(s):  
Jion M. Battad ◽  
Daouda A. K. Traore ◽  
Emma Byres ◽  
Jamie Rossjohn ◽  
Rodney J. Devenish ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Optical Properties ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
X Ray ◽  
Green Fluorescent

scholarly journals The yeast peroxisomal adenine nucleotide transporter: characterization of two transport modes and involvement in ΔpH formation across peroxisomal membranes

2004 ◽  
Vol 381 (3) ◽  
pp. 581-585 ◽  
Author(s):  
Francesco M. LASORSA ◽  
Pasquale SCARCIA ◽  
Ralf ERDMANN ◽  
Ferdinando PALMIERI ◽  
Hanspeter ROTTENSTEINER ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescence Imaging ◽  
Fluorescent Protein ◽  
Adenine Nucleotide ◽  
Ph Sensitive ◽  
Peroxisomal Membrane ◽  
Transport Modes ◽  
Proton Movement ◽  
Green Fluorescent

The yeast peroxisomal adenine nucleotide carrier, Ant1p, was shown to catalyse unidirectional transport in addition to exchange of substrates. In both transport modes, proton movement occurs. Nucleotide hetero-exchange is H+-compensated and electroneutral. Furthermore, microscopic fluorescence imaging of a pH-sensitive green fluorescent protein targeted to peroxisomes shows that Ant1p is involved in the formation of a ΔpH across the peroxisomal membrane, acidic inside.

scholarly journals Cathodoluminescence of green fluorescent protein exhibits the redshifted spectrum and the robustness

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Keiichirou Akiba ◽  
Katsuyuki Tamehiro ◽  
Koki Matsui ◽  
Hayata Ikegami ◽  
Hiroki Minoda
Keyword(s):  
Optical Properties ◽  
Electron Beam ◽  
Electron Microscope ◽  
Green Fluorescent Protein ◽  
Electron Beam Irradiation ◽  
Fluorescent Protein ◽  
Structural Information ◽  
Ion Beam ◽  
Beam Irradiation ◽  
Green Fluorescent

Abstract Green fluorescent protein (GFP) and its variants are an essential tool for visualizing functional units in biomaterials. This is achieved by the fascinating optical properties of them. Here, we report novel optical properties of enhanced GFP (EGFP), which is one of widely used engineered variants of the wild-type GFP. We study the electron-beam-induced luminescence, which is known as cathodoluminescence (CL), using the hybrid light and transmission electron microscope. Surprisingly, even from the same specimen, we observe a completely different dependences of the fluorescence and CL on the electron beam irradiation. Since light emission is normally independent of whether an electron is excited to the upper level by light or by electron beam, this difference is quite peculiar. We conclude that the electron beam irradiation causes the local generation of a new redshifted form of EGFP and CL is preferentially emitted from it. In addition, we also find that the redshifted form is rather robust to electron bombardment. These remarkable properties can be utilized for three-dimensional reconstruction without electron staining in focused ion beam/scanning electron microscopy technology and provide significant potential for simultaneously observing the functional information specified by super-resolution CL imaging and the structural information at the molecular level obtained by electron microscope.

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