A microfluidic device for physical trapping and electrical lysis of bacterial cells

2008 ◽  
Vol 92 (21) ◽  
pp. 214103 ◽  
Author(s):  
Ning Bao ◽  
Chang Lu
Keyword(s):  
Microfluidic Device ◽  
Bacterial Cells ◽  
Electrical Lysis

Rapid Electrical Lysis of Bacterial Cells in a Microfluidic Device

2007 ◽  
pp. 23-35 ◽  
Author(s):  
Hsiang-Yu Wang ◽  
Padmapriya P. Banada ◽  
Arun K. Bhunia ◽  
Chang Lu
Keyword(s):  
Microfluidic Device ◽  
Bacterial Cells ◽  
Electrical Lysis

Mechanism of Cell Lysis in Microfluidic Channel With Integrated Nanocomposite Electrodes

2013 ◽  
Author(s):  
Madhusmita Mishra ◽  
Anil Krishna Koduri ◽  
Aman Chandra ◽  
D. Roy Mahapatra ◽  
G. M. Hegde
Keyword(s):  
Electric Field ◽  
Microfluidic Channel ◽  
Cell Lysis ◽  
Bacterial Cells ◽  
Nano Composite ◽  
Time Resolved ◽  
Ac Electric Field ◽  
Cell Dispersion ◽  
Electrical Lysis ◽  
Nanocomposite Electrodes

This paper reports on the characterization of an integrated micro-fluidic platform for controlled electrical lysis of biological cells and subsequent extraction of intracellular biomolecules. The proposed methodology is capable of high throughput electrical cell lysis facilitated by nano-composite coated electrodes. The nano-composites are synthesized using Carbon Nanotube and ZnO nanorod dispersion in polymer. Bacterial cells are used to demonstrate the lysis performance of these nanocomposite electrodes. Investigation of electrical lysis in the microchannel is carried out under different parameters, one with continuous DC application and the other under DC biased AC electric field. Lysis in DC field is dependent on optimal field strength and governed by the cell type. By introducing the AC electrical field, the electrokinetics is controlled to prevent cell clogging in the micro-channel and ensure uniform cell dispersion and lysis. Lysis mechanism is analyzed with time-resolved fluorescence imaging which reveal the time scale of electrical lysis and explain the dynamic behavior of GFP-expressing E. coli cells under the electric field induced by nanocomposite electrodes. The DNA and protein samples extracted after lysis are compared with those obtained from a conventional chemical lysis method by using a UV–Visible spectroscopy and fluorimetry. The paper also focuses on the mechanistic understanding of the nano-composite coating material and the film thickness on the leakage charge densities which lead to differential lysis efficiency.

scholarly journals Electrophoretic Concentration and Electrical Lysis of Bacteria in a Microfluidic Device Using a Nanoporous Membrane

Micromachines ◽  
2017 ◽  
Vol 8 (2) ◽  
pp. 45 ◽  
Author(s):  
Md. Islam ◽  
Ali Shahid ◽  
Kacper Kuryllo ◽  
Yingfu Li ◽  
M. Deen ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Nanoporous Membrane ◽  
Electrophoretic Concentration ◽  
Electrical Lysis

scholarly journals Microfluidic Device for Automated Synchronization of Bacterial Cells

2012 ◽  
Vol 84 (20) ◽  
pp. 8571-8578 ◽  
Author(s):  
Seth M. Madren ◽  
Michelle D. Hoffman ◽  
Pamela J. B. Brown ◽  
David T. Kysela ◽  
Yves V. Brun ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Bacterial Cells

Rapid quantification of bacterial cells in potable water using a simplified microfluidic device

2007 ◽  
Vol 68 (3) ◽  
pp. 643-647 ◽  
Author(s):  
Chieko Sakamoto ◽  
Nobuyasu Yamaguchi ◽  
Masumi Yamada ◽  
Hiroyasu Nagase ◽  
Minoru Seki ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Potable Water ◽  
Bacterial Cells ◽  
Rapid Quantification

scholarly journals Statistical optimization of the lysis agents for gram-negative bacterial cells in a microfluidic device

2006 ◽  
Vol 11 (6) ◽  
pp. 557-557
Author(s):  
Young-Bum Kim ◽  
Ji-Ho Park ◽  
Woo-Jin Chang ◽  
Yoon-Mo Koo ◽  
Eun-Ki Kim ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Statistical Optimization ◽  
Bacterial Cells ◽  
Gram Negative

scholarly journals Rapid, Semiautomated Quantification of Bacterial Cells in Freshwater by Using a Microfluidic Device for On-Chip Staining and Counting

2010 ◽  
Vol 77 (4) ◽  
pp. 1536-1539 ◽  
Author(s):  
Nobuyasu Yamaguchi ◽  
Masashi Torii ◽  
Yuko Uebayashi ◽  
Masao Nasu
Keyword(s):  
Escherichia Coli ◽  
Microfluidic Device ◽  
Potable Water ◽  
Pond Water ◽  
Bacterial Cells ◽  
On Chip

ABSTRACTA microfluidic device-based system for the rapid and semiautomated counting of bacteria in freshwater was fabricated and examined. Bacteria in groundwater and in potable water, as well as starvedEscherichia coliO157:H7 spiked in pond water, were able to be on-chip stained and enumerated within 1 h using this system.

Selective isolation of bacterial cells within a microfluidic device using magnetic probe-based cell fishing

2014 ◽  
Vol 195 ◽  
pp. 581-589 ◽  
Author(s):  
Jérémy Pivetal ◽  
Sylvain Toru ◽  
Marie Frenea-Robin ◽  
Naoufel Haddour ◽  
Sébastien Cecillon ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Magnetic Probe ◽  
Bacterial Cells ◽  
Selective Isolation
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