A microfluidic flow-through device for high throughput electrical lysis of bacterial cells based on continuous dc voltage

2006 ◽  
Vol 22 (5) ◽  
pp. 582-588 ◽  
Author(s):  
Hsiang-Yu Wang ◽  
Arun K. Bhunia ◽  
Chang Lu
Keyword(s):  
High Throughput ◽  
Bacterial Cells ◽  
Dc Voltage ◽  
Microfluidic Flow ◽  
Electrical Lysis ◽  
Flow Through

Mechanism of Cell Lysis in Microfluidic Channel With Integrated Nanocomposite Electrodes

2013 ◽  
Author(s):  
Madhusmita Mishra ◽  
Anil Krishna Koduri ◽  
Aman Chandra ◽  
D. Roy Mahapatra ◽  
G. M. Hegde
Keyword(s):  
Electric Field ◽  
Microfluidic Channel ◽  
Cell Lysis ◽  
Bacterial Cells ◽  
Nano Composite ◽  
Time Resolved ◽  
Ac Electric Field ◽  
Cell Dispersion ◽  
Electrical Lysis ◽  
Nanocomposite Electrodes

This paper reports on the characterization of an integrated micro-fluidic platform for controlled electrical lysis of biological cells and subsequent extraction of intracellular biomolecules. The proposed methodology is capable of high throughput electrical cell lysis facilitated by nano-composite coated electrodes. The nano-composites are synthesized using Carbon Nanotube and ZnO nanorod dispersion in polymer. Bacterial cells are used to demonstrate the lysis performance of these nanocomposite electrodes. Investigation of electrical lysis in the microchannel is carried out under different parameters, one with continuous DC application and the other under DC biased AC electric field. Lysis in DC field is dependent on optimal field strength and governed by the cell type. By introducing the AC electrical field, the electrokinetics is controlled to prevent cell clogging in the micro-channel and ensure uniform cell dispersion and lysis. Lysis mechanism is analyzed with time-resolved fluorescence imaging which reveal the time scale of electrical lysis and explain the dynamic behavior of GFP-expressing E. coli cells under the electric field induced by nanocomposite electrodes. The DNA and protein samples extracted after lysis are compared with those obtained from a conventional chemical lysis method by using a UV–Visible spectroscopy and fluorimetry. The paper also focuses on the mechanistic understanding of the nano-composite coating material and the film thickness on the leakage charge densities which lead to differential lysis efficiency.

scholarly journals A Continuous Flow-through Microfluidic Device for Electrical Lysis of Cells

Micromachines ◽  
2019 ◽  
Vol 10 (4) ◽  
pp. 247 ◽  
Author(s):  
Ying-Jie Lo ◽  
U Lei
Keyword(s):  
Electric Field ◽  
Cross Flow ◽  
Electrode Array ◽  
Threshold Value ◽  
Lower Half ◽  
Rectangular Microchannel ◽  
Peak Voltage ◽  
Biomedical Systems ◽  
Electrical Lysis ◽  
Flow Through

In contrast to the delicate 3D electrodes in the literature, a simple flow-through device is proposed here for continuous and massive lysis of cells using electricity. The device is essentially a rectangular microchannel with a planar electrode array built on its bottom wall, actuated by alternating current (AC) voltages between neighboring electrodes, and can be incorporated easily into other biomedical systems. Human whole blood diluted 10 times with phosphate-buffered saline (about 6 × 108 cells per mL) was pumped through the device, and the cells were completely lysed within 7 s after the application of a 20 V peak-to-peak voltage at 1 MHz, up to 400 μL/hr. Electric field and Maxwell stress were calculated for assessing electrical lysis. Only the lower half-channel was exposed to an electric field exceeding the irreversible threshold value of cell electroporation (Eth2), suggesting that a cross flow, proposed here primarily as the electro-thermally induced flow, was responsible for bringing the cells in the upper half-channel downward to the lower half-channel. The Maxwell shear stress associated with Eth2 was one order of magnitude less than the threshold mechanical stresses for lysis, implying that an applied moderate mechanical stress could aid electrical lysis.

scholarly journals Assessing Adhesion Forces of Type I and Type IV Pili of Xylella fastidiosa Bacteria by Use of a Microfluidic Flow Chamber

2007 ◽  
Vol 73 (8) ◽  
pp. 2690-2696 ◽  
Author(s):  
Leonardo De La Fuente ◽  
Emilie Montanes ◽  
Yizhi Meng ◽  
Yaxin Li ◽  
Thomas J. Burr ◽  
...  
Keyword(s):  
Adhesion Force ◽  
Xylella Fastidiosa ◽  
Flow Chamber ◽  
Type Iv Pili ◽  
Type I ◽  
Bacterial Cells ◽  
Adhesion Forces ◽  
Type Iv ◽  
Microfluidic Flow ◽  
Biofilm Development

ABSTRACT Xylella fastidiosa, a bacterium responsible for Pierce's disease in grapevines, possesses both type I and type IV pili at the same cell pole. Type IV pili facilitate twitching motility, and type I pili are involved in biofilm development. The adhesiveness of the bacteria and the roles of the two pili types in attachment to a glass substratum were evaluated using a microfluidic flow chamber in conjunction with pilus-defective mutants. The average adhesion force necessary to detach wild-type X. fastidiosa cells was 147 ± 11 pN. Mutant cells possessing only type I pili required a force of 204 ± 22 pN for removal, whereas cells possessing only type IV pili required 119 ± 8 pN to dislodge these cells. The experimental results demonstrate that microfluidic flow chambers are useful and convenient tools for assessing the drag forces necessary for detaching bacterial cells and that with specific pilus mutants, the role of the pilus type can be further assessed.

scholarly journals High-Throughput Microfiltration Membranes with Natural Biofouling Reducer Agent for Food Processing

Processes ◽  
2018 ◽  
Vol 7 (1) ◽  
pp. 1 ◽  
Author(s):  
Panggulu Utoro ◽  
Agung Sukoyo ◽  
Sandra Sandra ◽  
Nimatul Izza ◽  
Shinta Dewi ◽  
...  
Keyword(s):  
Cellulose Acetate ◽  
High Throughput ◽  
Water Flux ◽  
Antibacterial Properties ◽  
Mixed Matrix Membranes ◽  
Bacterial Cells ◽  
E Coli ◽  
Membrane Surfaces ◽  
Cellulose Acetate Membranes ◽  
Microfiltration Membranes

The effect of natural antibiotics Moringa oleifera seeds powder in cellulose acetate membranes as biofouling reducer agent was investigated. Mixed matrix membranes (MMM) were synthesized by adding 100 mesh M. oleifera seeds powder with variation of three concentrations (1 wt%, 2 wt%, and 3 wt%), into a mix polymer solution of CA (cellulose acetate) and two different solvents, i.e., DMF (dimethylformamide) and DMAc (dimethylacetamide). The synthesized membranes morphology was observed under scanning electron microscopy and from the images can be seen that the membranes made of DMAc formed rather large macrovoid as compared to DMF-based membranes. The microstructure affected the water flux through the membranes, in which the DMAc membranes provided a higher flux value and served as high-throughput microfiltration membranes. Antibacterial properties of MMM were tested using Escherichia coli adhesion onto membrane surfaces. The results showed that M. oleifera has been proven to eradicate E. coli activity on the membrane surfaces due to interaction between bacterial cells and phenolic compounds from M. oleifera, through absorption processes involving hydrogen bonds.

scholarly journals In situ capabilities of Small Angle X-ray Scattering

2019 ◽  
Vol 8 (1) ◽  
pp. 352-369 ◽  
Author(s):  
Jinghua Feng ◽  
Manfred Kriechbaum ◽  
Li (Emily) Liu
Keyword(s):  
Magnetic Field ◽  
High Throughput ◽  
Small Angle ◽  
Nanoscale Systems ◽  
X Ray ◽  
X Ray Scattering ◽  
Flow Through ◽  
Stretching Flow ◽  
Ray Scattering

Abstract Small Angle X-ray Scattering (SAXS) is an ideal characterization tool to explore nanoscale systems. In order to investigate nanostructural changes of materials under realistic sample environments, it is essential to equip SAXS with diverse in situ capabilities based on the corresponding requirements. In this paper, we highlight the representative experimental setups and corresponding applications of five widely used in situ capabilities: temperature, pressure, stretching, flow-through, and electric field. Additionally, we also briefly introduce other four in situ techniques including humidity, high-throughput, rheology, and magnetic field.

Metal Nanowire Felt as a Flow-Through Electrode for High-Throughput Electrosynthesis

2018 ◽  
Author(s):  
Myung Jun Kim ◽  
Youngran Seo ◽  
Mutya Cruz ◽  
Benjamin Wiley
Keyword(s):  
Charge Transfer ◽  
Mass Transport ◽  
Intramolecular Cyclization ◽  
High Throughput ◽  
Cyclization Reaction ◽  
Carbon Paper ◽  
Organic Chemicals ◽  
Flow Through ◽  
Cu Ions ◽  
Metal Nanowire

The low productivity of organic electrosynthesis limits the adoption of this green methodology for production of organic chemicals. This work examines to what extent reducing the size of the fibers in a flow-through electrode to the nanoscale can increase the productivity of electrosynthesis. A Cu nanowire felt, made from nanowires 45 times smaller than the 10-μm-wide fibers in carbon paper, achieved a productivity 278 times higher than carbon paper for mass transport-limited reduction of Cu ions. For an intramolecular cyclization reaction that was limited by both mass and charge transfer kinetics, the Cu nanowire felt achieved a productivity 4.2 times higher than carbon paper. This work demonstrates large gains in productivity can be achieved with nanostructured flow-through electrodes, but that potential gains can be limited by charge transfer kinetics.

A DC Voltage Driven Flow-Through Electroporation Microchip

2009 ◽  
Vol 60-61 ◽  
pp. 44-48
Author(s):  
Qiao Le Zhao ◽  
Yu Cheng Lin ◽  
Qin Gan Huang
Keyword(s):  
Field Strength ◽  
Electric Field ◽  
Shape Change ◽  
Low Cost ◽  
Strength Distribution ◽  
Time Span ◽  
Dc Voltage ◽  
Flow Through ◽  
Set Up ◽  
Field Strengths

Differing with the traditional way to perform electroporation (EP) by using the DC electrical pulse, this paper proposes a new EP system by applying continuous DC voltages to generate proper EP electric field strengths utilizing the shape change of the channel. The fabrication of chip and set-up of system are clearly described and simulations also carried out utilizing CFD-ACE to study the electric field strength distribution and the time span when fluid passes through different electric field strengths. The fabrication of the proposed EP system is quite simple and low-cost.

Sign in / Sign up

Export Citation Format

Share Document