L-band electron paramagnetic resonance spectrometer for use in vivo and in studies of aqueous biological samples

2005 ◽  
Vol 76 (1) ◽  
pp. 013107 ◽  
Author(s):  
T. Walczak ◽  
P. Leśniewski ◽  
I. Salikhov ◽  
A. Sucheta ◽  
K. Szybiński ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Biological Samples ◽  
Electron Paramagnetic Resonance Spectrometer ◽  
Paramagnetic Resonance ◽  
Band Electron ◽  
L Band ◽  
Electron Paramagnetic

scholarly journals Developments in Biodosimetry Methods for Triage With a Focus on X-band Electron Paramagnetic Resonance In Vivo Fingernail Dosimetry

2018 ◽  
Vol 115 (1) ◽  
pp. 140-150 ◽  
Author(s):  
Steven G. Swarts ◽  
Jason W. Sidabras ◽  
Oleg Grinberg ◽  
Dmitriy S. Tipikin ◽  
Maciej M. Kmiec ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Paramagnetic Resonance ◽  
Band Electron ◽  
X Band ◽  
Electron Paramagnetic ◽  
Fingernail Dosimetry

scholarly journals Detection of L-band electron paramagnetic resonance in the DPPH molecule using impedance measurements

RSC Advances ◽  
2020 ◽  
Vol 10 (29) ◽  
pp. 17311-17316
Author(s):  
Ushnish Chaudhuri ◽  
R. Mahendiran
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Schematic Diagram ◽  
Paramagnetic Resonance ◽  
Impedance Measurements ◽  
Band Electron ◽  
L Band ◽  
Set Up ◽  
Electron Paramagnetic ◽  
Strip Coil

(a) Schematic diagram of our experimental set up. (b) Resistance and reactance of the DPPH molecule for 2 GHz current in the strip coil.

In vivo imaging of oxidative stress in ischemia-reperfusion renal injury using electron paramagnetic resonance

2005 ◽  
Vol 288 (3) ◽  
pp. F597-F603 ◽  
Author(s):  
Aki Hirayama ◽  
Sohji Nagase ◽  
Atsushi Ueda ◽  
Takaaki Oteki ◽  
Kenji Takada ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Electron Paramagnetic Resonance ◽  
Serum Creatinine ◽  
Ischemia Reperfusion ◽  
Partial Recovery ◽  
Paramagnetic Resonance ◽  
Reducing Activity ◽  
L Band ◽  
Electron Paramagnetic

Oxidative stress during ischemia-reperfusion acute renal failure (IR-ARF) was noninvasively evaluated with in vivo electron paramagnetic resonance (EPR) imaging. Female ICR mice underwent left nephrectomy and 30-min ischemia-reperfusion of the right kidney. Oxidative stress was evaluated as organ reducing activity with the half-lives of the spin probe 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (carbamoyl-PROXYL) using 1) conventional L-band EPR, which showed organ-reducing activity in the whole abdominal area; and 2) EPR imaging, which showed semiquantitative but organ-specific reducing activity. The results were compared with the reducing activity of organ homogenate and phosphatidylcholine hydroperoxide (PC-OOH) concentrations. Half-lives of carbamoyl-PROXYL in the whole upper abdominal area, measured by L-band EPR, were prolonged on day 3 after ischemia-reperfusion and recovered to the level of nontreated mice on day 7. This trend resembled closely that of serum creatinine and blood urea nitrogen concentration. The EPR imaging-measured carbamoyl-PROXYL half-life was also prolonged on day 3 in both the kidney and the liver. However, in the kidney this showed only partial recovery on day 7. In the liver, this convalescence was more remarkable. The ex vivo studies of organ reducing activity and PC-OOH agreed with the results from EPRI, but not with those from L-band EPR. These results indicate that renal reducing activity shows only partial recovery on day 7 after ischemia-reperfusion, when serum creatinine and blood urea nitrogen have recovered. EPR imaging is an appropriate and useful method for the noninvasive evaluation of oxidative stress in the presence of renal injury.

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