On the Size, Shape, and Hydration of Southern Bean Mosaic Virus and Tobacco Necrosis Virus in Solution

1951 ◽  
Vol 19 (6) ◽  
pp. 793-794 ◽  
Author(s):  
B. R. Leonard ◽  
J. W. Anderegg ◽  
Paul Kaesberg ◽  
S. Shulman ◽  
W. W. Beeman
1966 ◽  
Vol 44 (6) ◽  
pp. 821-826 ◽  
Author(s):  
J. R. Edwardson ◽  
D. E. Purcifull ◽  
R. G. Christie

Particles within lesions of leaf tissue infected with either tobacco necrosis virus (TNV) or southern bean mosaic virus (SBMV) were compared with particles in embedded pellets of purified preparations of these viruses by an examination of thin sections. The mode of the diameters of particles in tissues and pellets was 20.5 mµ.It is assumed that the particles in infected tissues are virus particles on the basis of their similarities in size, shape, and arrangement with the particles in purified preparations.


Parasitology ◽  
1941 ◽  
Vol 33 (3) ◽  
pp. 320-330 ◽  
Author(s):  
Kenneth M. Smith ◽  
W. D. MacClement

An account is given of ultrafiltration studies with 9 plant viruses. It is shown that 3 of these viruses filter in a consistent manner and appear to have approximately spherical particles. These three are Lycopersicum virus 4 (tomato bushy stunt virus), Nicotiana virus 11 (tobacco necrosis virus) and Nicotiana virus 12 (tobacco ringspot virus). The filtration end-point of 40 mμ is the same in each case and from this a particle diameter of 13–20 mμ is calculated. There is a peculiarity, however, in the filtration curve of tobacco necrosis virus which shows itself in a “bench” or “shelf” and which suggests either a polydisperse system or some degree of dissymmetry of particle shape.Great difficulty was experienced in filtering Nicotiana virus 1 (tobacco mosaic virus) and its strains. A value of 13–20 mμ was obtained for the particle diameter of the type virus and this agrees well with measurements obtained by other methods. The filtration results, however, suggest that the infective units are not of the same length and that this variability may be considerable. Similar difficulty was experienced in filtering Solanum virus 1 (potato virus X), another rod-shaped virus; the end-point was found to be 100 mμ, from which a particle diameter of 33–50 mμ is calculated. It was not possible to obtain a definite filtration end-point for Cucumis virus 1 (cucumber mosaic virus), probably because of the low initial concentration of virus in the extracted sap.


PLoS ONE ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. e0128171
Author(s):  
Carla M. R. Varanda ◽  
Marco Machado ◽  
Paulo Martel ◽  
Gustavo Nolasco ◽  
Maria I. E. Clara ◽  
...  

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