Changes in Composition during Electron Micro‐Probe Analysis of K2O–SrO–SiO2 Glass

1966 ◽  
Vol 37 (5) ◽  
pp. 2199-2199 ◽  
Author(s):  
A. K. Varshneya ◽  
A. R. Cooper ◽  
M. Cable
Author(s):  
A. V. Somlyo ◽  
H. Shuman ◽  
A. P. Somlyo

Electron probe analysis of frozen dried cryosections of frog skeletal muscle, rabbit vascular smooth muscle and of isolated, hyperpermeab1 e rabbit cardiac myocytes has been used to determine the composition of the cytoplasm and organelles in the resting state as well as during contraction. The concentration of elements within the organelles reflects the permeabilities of the organelle membranes to the cytoplasmic ions as well as binding sites. The measurements of [Ca] in the sarcoplasmic reticulum (SR) and mitochondria at rest and during contraction, have direct bearing on their role as release and/or storage sites for Ca in situ.


Author(s):  
Delbert E. Philpott ◽  
David Leaffer

There are certain advantages for electron probe analysis if the sample can be tilted directly towards the detector. The count rate is higher, it optimizes the geometry since only one angle need be taken into account for quantitative analysis and the signal to background ratio is improved. The need for less tilt angle may be an advantage because the grid bars are not moved quite as close to each other, leaving a little more open area for observation. Our present detector (EDAX) and microscope (Philips 300) combination precludes moving the detector behind the microscope where it would point directly at the grid. Therefore, the angle of the specimen was changed in order to optimize the geometry between the specimen and the detector.


Author(s):  
Avril V. Somlyo ◽  
H. Shuman ◽  
A.P. Somlyo

This is a preliminary report of electron probe analysis of rabbit portal-anterior mesenteric vein (PAMV) smooth muscle cryosectioned without fixation or cryoprotection. The instrumentation and method of electron probe quantitation used (1) and our initial results with cardiac (2) and skeletal (3) muscle have been presented elsewhere.In preparations depolarized with high K (K2SO4) solution, significant calcium peaks were detected over the sarcoplasmic reticulum (Fig 1 and 2) and the continuous perinuclear space. In some of the fibers there were also significant (up to 200 mM/kg dry wt) calcium peaks over the mitochondria. However, in smooth muscle that was not depolarized, high mitochondrial Ca was found in fibers that also contained elevated Na and low K (Fig 3). Therefore, the possibility that these Ca-loaded mitochondria are indicative of cell damage remains to be ruled out.


Author(s):  
T.R. Dinger ◽  
G. Thomas

The use of Si3N4, alloys for high temperature, high stress structural applications has prompted numerous studies of the oxynitride glasses which exist as intergranular phases in their microstructures. Oxynitride glasses have been investigated recently in their bulk form in order to understand their crystallization behavior for subsequent Si3N4 applications and to investigate their worth as glass-ceramic precursors. This research investigates the crystallization sequence of a glass having a normalized composition of Y26Si30Al11 ON11 and lying in the A1N-Y2O3-SiO2 section of the Y-Si-Al-O-N system. Such glasses exist as intergranular phases in the technologically important Y2O3/Al2O3-fluxed Si3N4 alloys.


1987 ◽  
Vol 48 (C6) ◽  
pp. C6-349-C6-354
Author(s):  
K. Hono ◽  
T. Sakurai ◽  
H. W. Pickering

1988 ◽  
Vol 49 (C6) ◽  
pp. C6-299-C6-304 ◽  
Author(s):  
U. ROLANDER ◽  
H.-O. ANDRÉN

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