Current Fluctuation Phenomena in Current‐Carrying Sliding Contacts

1954 ◽  
Vol 25 (12) ◽  
pp. 1473-1480 ◽  
Author(s):  
G. W. Epprecht
Keyword(s):  
Fluctuation Phenomena ◽  
Sliding Contacts ◽  
Current Fluctuation

Small Molecule Permeation Across Membrane Channels: Chemical Modification to Quantify Transport Across OmpF

2018 ◽  
Author(s):  
Jiajun Wang ◽  
Jayesh Arun Bafna ◽  
Satya Prathyusha Bhamidimarri ◽  
Mathias Winterhalter
Keyword(s):  
Dwell Time ◽  
Covalent Binding ◽  
Channel Structure ◽  
Ion Current ◽  
E Coli ◽  
Current Fluctuation ◽  
Wide Range ◽  
Outer Membrane Channel ◽  
Biological Channels ◽  
Constriction Region

Biological channels facilitate the exchange of small molecules across membranes, but surprisingly there is a lack of general tools for the identification and quantification of transport (i.e., translocation and binding). Analyzing the ion current fluctuation of a typical channel with its constriction region in the middle does not allow a direct conclusion on successful transport. For this, we created an additional barrier acting as a molecular counter at the exit of the channel. To identify permeation, we mainly read the molecule residence time in the channel lumen as the indicator whether the molecule reached the exit of the channel. As an example, here we use the well-studied porin, OmpF, an outer membrane channel from <i>E. coli</i>. Inspection of the channel structure suggests that aspartic acid at position 181 is located below the constriction region (CR) and we subsequently mutated this residue to cysteine, where else cysteine free and functionalized it by covalent binding with 2-sulfonatoethyl methanethiosulfonate (MTSES) or the larger glutathione (GLT) blockers. Using the dwell time as the signal for transport, we found that both mono-arginine and tri-arginine permeation process is prolonged by 20% and 50% respectively through OmpF<sub>E181C</sub>MTSES, while the larger sized blocker modification OmpF<sub>E181C</sub>GLT drastically decreased the permeation of mono-arginine by 9-fold and even blocked the pathway of the tri-arginine. In case of the hepta-arginine as substrate, both chemical modifications led to an identical ‘blocked’ pattern observed by the dwell time of ion current fluctuation of the OmpF<sub>wt</sub>. As an instance for antibiotic permeation, we analyzed norfloxacin, a fluoroquinolone antimicrobial agent. The modulation of the interaction dwell time suggests possible successful permeation of norfloxacin across OmpF<sub>wt</sub>. This approach may discriminate blockages from translocation events for a wide range of substrates. A potential application could be screening for scaffolds to improve the permeability of antibiotics.

scholarly journals Sliding Contacts in Planetary Magnetospheres

Symmetry ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 283
Author(s):  
Elena Belenkaya ◽  
Igor Alexeev
Keyword(s):  
Large Scale ◽  
Strong Field ◽  
Magnetized Plasma ◽  
Sliding Contacts ◽  
Radio Emissions ◽  
Planetary Magnetospheres ◽  
Current Task ◽  
Surrounding Space ◽  
Non Local ◽  
A Current

In the planetary magnetospheres there are specific places connected with velocity breakdown, reconnection, and dynamo processes. Here we pay attention to sliding layers. Sliding layers are formed in the ionosphere, on separatrix surfaces, at the magnetopauses and boundaries of stellar astrospheres, and at the Alfvén radius in the equatorial magnetosphere of rapidly rotating strongly magnetized giant planets. Although sliding contacts usually occur in thin local layers, their influence on the global structure of the surrounding space is very great. Therefore, they are associated with non-local processes that play a key role on a large scale. There can be an exchange between different forms of energy, a generation of strong field-aligned currents and emissions, and an amplification of magnetic fields. Depending on the conditions in the magnetosphere of the planet/exoplanet and in the flow of magnetized plasma passing it, different numbers of sliding layers with different configurations appear. Some are associated with regions of auroras and possible radio emissions. The search for planetary radio emissions is a current task in the detection of exoplanets.

scholarly journals An Optimal Control Algorithm with Reduced DC-Bus Current Fluctuation for Multiple Charging Modes of Electric Vehicle Charging Station

2021 ◽  
Vol 12 (3) ◽  
pp. 107
Author(s):  
Tao Chen ◽  
Peng Fu ◽  
Xiaojiao Chen ◽  
Sheng Dou ◽  
Liansheng Huang ◽  
...  
Keyword(s):  
Electric Vehicle ◽  
Electric Vehicles ◽  
Control Algorithm ◽  
Current Fluctuation ◽  
Charging Station ◽  
Electric Vehicle Charging ◽  
Three Phase ◽  
Optimization Control ◽  
Dc Bus ◽  
Vehicle Charging

This paper presents a systematic structure and a control strategy for the electric vehicle charging station. The system uses a three-phase three-level neutral point clamped (NPC) rectifier to drive multiple three-phase three-level NPC converters to provide electric energy for electric vehicles. This topology can realize the single-phase AC mode, three-phase AC mode, and DC mode by adding some switches to meet different charging requirements. In the case of multiple electric vehicles charging simultaneously, a system optimization control algorithm is adopted to minimize DC-bus current fluctuation by analyzing and reconstructing the DC-bus current in various charging modes. This algorithm uses the genetic algorithm (ga) as the core of computing and reduces the number of change parameter variables within a limited range. The DC-bus current fluctuation is still minimal. The charging station system structure and the proposed system-level optimization control algorithm can improve the DC-side current stability through model calculation and simulation verification.

Multiscale Analysis on Friction-Reducing Characteristics of Textured Surface in Nanoscale Sliding Contacts

2011 ◽  
Vol 86 ◽  
pp. 649-652
Author(s):  
Rui Ting Tong ◽  
Geng Liu ◽  
Lan Liu ◽  
Shang Jun Ma
Keyword(s):  
Copper Substrate ◽  
Dynamics Simulation ◽  
Textured Surface ◽  
Face Centered Cubic ◽  
Textured Surfaces ◽  
Sliding Contacts ◽  
Friction Forces ◽  
Surface Textures ◽  
Force Reduction ◽  
Face Centered

A multiscale method coupled molecular dynamics simulation and finite element method is used to investigate two dimensional nanoscale sliding contacts between a rigid cylindrical tip and an elastic face centered cubic copper substrate with textured surface, in which adhesive effects are considered. Two series of nanoscale surface textures with different asperity shape, different asperity heights and different spacing between asperities are designed. Through the friction forces comparisons between smooth surface and textured surfaces, a better shape is advised to indicate that asperity shape plays an important role in friction force reduction. With proper asperity height and proper spacing between asperities, surface textures can reduce friction forces effectively.

scholarly journals Two ATP-activated conductances in bullfrog atrial cells.

1988 ◽  
Vol 91 (1) ◽  
pp. 1-27 ◽  
Author(s):  
D D Friel ◽  
B P Bean
Keyword(s):  
Ionic Channels ◽  
Extracellular Atp ◽  
Fluctuation Analysis ◽  
Current Fluctuation ◽  
Atrial Cells ◽  
Atp Analogues ◽  
Alpha Beta ◽  
Gamma S ◽  
Inwardly Rectifying ◽  
Current Fluctuation Analysis

Currents activated by extracellular ATP were studied in single voltage-clamped bullfrog atrial cells. Rapid application of ATP elicited currents carried through two different conductance pathways: a rapidly desensitizing conductance reversing near -10 mV, and a maintained, inwardly rectifying conductance reversing near -85 mV. ATP activated the desensitizing component of current with a K 1/2 of approximately 50 microM and the maintained component with a K 1/2 of approximately 10 microM. Both types of current were activated by ATP but not by adenosine, AMP, or ADP. The desensitizing current was selectively inhibited by alpha, beta-methylene ATP, and the maintained, inwardly rectifying current was selectively suppressed by extracellular Cs. The desensitizing component of current was greatly reduced when extracellular Na was replaced by N-methylglucamine, but was slightly augmented when Na was replaced by Cs. GTP, ITP, and UTP were all ineffective in activating the desensitizing current, and of a variety of ATP analogues, only ATP-gamma-S was effective. Addition of EGTA or BAPTA to the intracellular solution did not obviously affect the desensitizing current. Fluctuation analysis of currents through the desensitizing conductance suggested that current is carried through ionic channels with a small (less than pS) unitary conductance.

Sign in / Sign up

Export Citation Format

Share Document