Peak Height Measurement System for Pulsed Laser Experiments

Abstract We describe a procedure for estimating mannitol concentrations in biological fluids. Samples are mixed with internal standard solution (alpha-methylglucose), deproteinized if necessary, desalted, and dried. Specimens are then derivatized by adding pyridine/bis(trimethylsilyl)acetamide/trimethylchlorosilane and heating at 60 degrees C for 30 min. Samples are chromatographed on a 275-cm column of 10% OV-17, operated at 190 degrees C, and quantitated by peak-height measurement. The technique is linear, accurate, precise, sensitive, and free from interference. It has been used to measure mannitol in plasma, urine, and bile.

Download Full-text

A Height Measurement Based on Laser Triangulation Probe and a Two Dimensional Image Measurement System for Smart Manufacturing

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.764-765.1324 ◽

2015 ◽

Vol 764-765 ◽

pp. 1324-1328

Author(s):

Pen Han Chen ◽

Chien Hung Liu ◽

Yu Fen Chen ◽

Hung Sheng Chiu ◽

Yu Chi Liu

Keyword(s):

Measurement System ◽

Smart Manufacturing ◽

Measuring Error ◽

Laser Triangulation ◽

Two Dimensional ◽

Image Measurement ◽

Height Measurement ◽

Dimensional Image ◽

Two Dimensional Image ◽

Auto Focusing

This paper integrated a laser triangulation probe with a two-dimensional image measurement system to measure the height of an object using the auto-focusing method. In the laser triangulation probe, the laser diode was used as the light source and one-dimensional position sensitive detector was adopted to receive the position of the laser spots to detect the height. The laser triangulation probe was used for small height measurement and the auto-focusing method was used for large height measurement. Two standard gauges with thicknesses of 8mm and 8.5mm were used to verify our proposed method. With height difference of 500μm, the experiment results showed the measuring error was within ±3μm and standard deviation was about 0.1μm.

Download Full-text

Quantitative Estimation of Clinically Important Monosaccharides in Plasma by Rapid Thin Layer Chromatography

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/000456327801500116 ◽

1978 ◽

Vol 15 (1-6) ◽

pp. 65-76 ◽

Cited By ~ 68

Author(s):

I. S. Menzies ◽

J. N. Mount ◽

M. J. Wheeler

Keyword(s):

Present Method ◽

Quantitative Estimation ◽

Peak Height ◽

Sample Volume ◽

Thin Layers ◽

The Other ◽

Initial Sample ◽

Height Measurement ◽

Internal Marker ◽

Layer Chromatography

Summary A method is described for the quantitative estimation of clinically important monosaccharides in plasma or whole blood by direct densitometry of chromatographically-separated zones on silica gel layers. Simple modifications of technique originally introduced to improve the reproducibility of paper chromatography have now been adapted for thin layers. The present method is based on peak height measurement with an internal marker correction. Galactose, fructose, D-xylose, and 3-O-methyl glucose can be estimated in addition to glucose, either singly or in combination, within three or four hours, using an initial sample volume of 0·5 ml. With reasonable experience and skill a coefficient of variation of 3 to 6 %, depending on sugar concentration, can be achieved without replication, and the limit of sensitivity is about 0·05 mmol/l. When the performance was compared with an automated glucose oxidase/peroxidase system for glucose and the recovery for the other monosaccharides was calculated, the results were satisfactory.

Download Full-text