On the efficiency of energy transfer in collisional activation of small peptides

2002 ◽  
Vol 116 (10) ◽  
pp. 4302-4310 ◽  
Author(s):  
Julia Laskin ◽  
Jean H. Futrell
Keyword(s):  
Energy Transfer ◽  
Collisional Activation ◽  
Small Peptides

Collisional activation of large ions. Energy losses and an impulsive collision theory of energy transfer

1994 ◽  
Vol 90 (2) ◽  
pp. 239 ◽  
Author(s):  
Caroline D. Bradley ◽  
Jonathan M. Curtis ◽  
Peter J. Derrick ◽  
Margaret M. Sheil
Keyword(s):  
Energy Transfer ◽  
Collisional Activation ◽  
Energy Losses ◽  
Collision Theory

Mechanism of Energy Transfer in Collisional Activation of Kiloelectron-Volt Macromolecular Ions

1989 ◽  
Vol 44 (3) ◽  
pp. 245-246 ◽  
Author(s):  
Einar Uggerud ◽  
Peter J. Derrick
Keyword(s):  
Energy Transfer ◽  
Collisional Activation ◽  
Collision Theory

An impulsive collision theory explains why helium is an effective target gas for collisionally activated decomposition of large biological ions.

Collisional Activation of Small Peptides

1999 ◽  
Vol 103 (20) ◽  
pp. 3981-3990 ◽  
Author(s):  
Oussama Meroueh ◽  
William L. Hase
Keyword(s):  
Collisional Activation ◽  
Small Peptides

A quantitative approach to inner shell losses

1978 ◽  
Vol 36 (1) ◽  
pp. 526-527 ◽  
Author(s):  
R.D. Leapman ◽  
P. Rez ◽  
D.F. Mayers
Keyword(s):  
Energy Transfer ◽  
Cross Section ◽  
Cross Sections ◽  
Accurate Determination ◽  
Background Intensity ◽  
Core Excitation ◽  
Quantitative Basis ◽  
Cross Section Differential ◽  
Bound Electrons

Microanalysis by EELS has been developing rapidly and though the general form of the spectrum is now understood there is a need to put the technique on a more quantitative basis (1,2). Certain aspects important for microanalysis include: (i) accurate determination of the partial cross sections, σx(α,ΔE) for core excitation when scattering lies inside collection angle a and energy range ΔE above the edge, (ii) behavior of the background intensity due to excitation of less strongly bound electrons, necessary for extrapolation beneath the signal of interest, (iii) departures from the simple hydrogenic K-edge seen in L and M losses, effecting σx and complicating microanalysis. Such problems might be approached empirically but here we describe how computation can elucidate the spectrum shape.The inelastic cross section differential with respect to energy transfer E and momentum transfer q for electrons of energy E0 and velocity v can be written as

The extracellular matrix of echinoderm and amphibian eggs: Visualization in quick-frozen, deep-etched, and rotary-shadowed specimens

1990 ◽  
Vol 48 (3) ◽  
pp. 60-61
Author(s):  
Barry Bonnell ◽  
Carolyn Larabell ◽  
Douglas Chandler
Keyword(s):  
Extracellular Matrix ◽  
Plasma Membrane ◽  
Sea Urchin ◽  
Crystalline Structure ◽  
Cortical Granule ◽  
Two Dimensional ◽  
Small Peptides ◽  
Deep Etching ◽  
Quick Freezing ◽  
Acrosomal Reaction

Eggs of many species including those of echinoderms, amphibians and mammals exhibit an extensive extracellular matrix (ECM) that is important both in the reception of sperm and in providing a block to polyspermy after fertilization.In sea urchin eggs there are two distinctive coats, the vitelline layer which contains glycoprotein sperm receptors and the jelly layer that contains fucose sulfate glycoconjugates which trigger the acrosomal reaction and small peptides which act as chemoattractants for sperm. The vitelline layer (VL), as visualized by quick-freezing, deep-etching, and rotary-shadowing (QFDE-RS), is a fishnet-like structure, anchored to the plasma membrane by short posts. Orbiting above the VL are horizontal filaments which are thought to anchor the thicker jelly layer to the egg. Upon fertilization, the VL elevates and is transformed by cortical granule secretions into the fertilization envelope (FE). The rounded casts of microvilli in the VL are transformed into angular peaks and the envelope becomes coated inside and out with sheets of paracrystalline protein having a quasi-two dimensional crystalline structure.

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