The Regulation of Solasodine Production by Agrobacterium rhizogenes-Transformed Roots of Solanum aviculare

Planta Medica ◽  
2000 ◽  
Vol 66 (5) ◽  
pp. 448-451 ◽  
Author(s):  
Adriana Carla Cavalcante Argôlo ◽  
Barry Victor Charlwood ◽  
Marcia Pletsch
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Transformed Roots ◽  
Solanum Aviculare

A reliable and efficient protocol for induction of hairy roots in Agastache foeniculum

Biologia ◽  
2014 ◽  
Vol 69 (7) ◽  
Author(s):  
Elnaz Nourozi ◽  
Bahman Hosseini ◽  
Abbas Hassani
Keyword(s):  
Salicylic Acid ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
High Performance ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Biochemical Properties ◽  
Separate Experiment ◽  
Transformed Roots

AbstractHairy root culture system is a valuable tool to study the characteristics of gene expression, gene function, root biology, biochemical properties and biosynthesis pathways of secondary metabolites. In the present study, hairy roots were established in Anise hyssop (Agastache foeniculum) via Agrobacterium rhizogenes. Three strains of Agrobacterium rhizogenes (A4, A7 and 9435), were used for induction of hairy roots in four various explants (hypocotyl, cotyledon, one-month-old leaf and five-month-old leaf) of Anise hyssop. The highest frequency of transformation was achieved using A4 strain in one-month-old leaves (51.1%). The transgenic states of hairy root lines were confirmed by PCR (Polymerase chain reaction) method. High performance liquid chromatography analysis revealed that the production of rosmarinic acid (RA) in transformed roots of A. foeniculum was almost 4-fold higher than that of the non-transformed roots. In a separate experiment, hairy roots obtained from one-month-old leaves inoculated with A4 strain, were grown in liquid medium and the effects of different concentrations of salicylic acid (0.0, 0.01, 0.1 and 1 mM) and chitosan (0, 50, 100 and 150 mg L−1) (as elicitor) and sucrose (20, 30, 40 and 50 g L−1) on the growth of hairy roots were evaluated. The results showed that, 30 g L−1 sucrose and 100 mg L−1 chitosan increased the biomass of hairy root cultures and application of salicylic acid reduced the growth of hairy roots compared with control roots.

scholarly journals Transgenic root cultures of Gentiana punctata L.

2014 ◽  
Vol 68 (4) ◽  
pp. 275-280 ◽  
Author(s):  
Branka Vinterhalter ◽  
Vladimir Orbović ◽  
Dragan Vinterhalter
Keyword(s):  
Growth Rate ◽  
Agrobacterium Rhizogenes ◽  
Basal Medium ◽  
Shoot Cultures ◽  
Root Cultures ◽  
Transformed Roots ◽  
Flower Buds ◽  
Transgenic Root ◽  
Short Internodes

Shoot cultures of <em>Gentiana punctata</em> L. were inoculated with suspension of <em>Agrobacterium rhizogenes</em> strain A4 M70GUS. Hairy roots which appeared 2-3 weeks later were cultured on hormone-free, liquid, WPM (Lloyd and McCown 1980) basal medium for more than 5 years (60 subcultures). Growth rate of transformed roots was higher than the growth rate of nontransformed roots. Spontaneous shoot regeneration occured only in three culture vessels in subcultures No. 40 and 42. Plants had phenotype characteristics typical for <em>A. rhizogenes</em> transformed plants including: wrincled leaves, short internodes, plagiotropic roots and in general their growth rate was reduced. These plants also manifested precocious formation of flower buds without vernalization and flowering under in vitro conditions. Flowers were pale yellow, the same as in the standard phenotype.

Induction of hairy roots in Arnica montana L. by Agrobacterium rhizogenes

2013 ◽  
Vol 8 (5) ◽  
pp. 470-479 ◽  
Author(s):  
Mariya Petrova ◽  
Ely Zayova ◽  
Mariana Vlahova
Keyword(s):  
Genetic Transformation ◽  
Agrobacterium Rhizogenes ◽  
Hairy Roots ◽  
Culture Media ◽  
Pcr Analysis ◽  
Transformed Roots ◽  
Fresh Weight ◽  
Transformation Techniques ◽  
Nutrient Media ◽  
Arnica Montana

AbstractThe induction of hairy roots in Arnica montana L. by Agrobacterium rhizogenes mediated system was established. The frequency of genetic transformation varied from 4.8 to 12% depended on method of infection. The cefotaxime at concentration of 200 mg/l proved to suppress effectively the growth of A. rhizogenes after co-cultivation. Among the three tested nutrient media: Murashige and Skoog (MS), Gamborg’s (B5) and Schenk and Hildebrandt (SH), MS medium was superior for growth and high biomass production of transformed roots compared to other culture media. After culturing for 40 days the fresh weight of clone T4 increased 7.6 fold over the non-transformed roots. The transfer of rol A, rol B and rol C genes into Arnica genome was confirmed by PCR analysis. Established genetic transformation techniques in A. montana efficiently provided and generated a large number of transformed roots — an excellent system for studying gene function and could be used for the production of secondary metabolites synthesized in roots.

Efficient production of Agrobacterium rhizogenes-transformed roots and composite plants for studying gene expression in coffee roots

2006 ◽  
Vol 25 (9) ◽  
pp. 959-967 ◽  
Author(s):  
E. Alpizar ◽  
E. Dechamp ◽  
S. Espeout ◽  
M. Royer ◽  
A. C. Lecouls ◽  
...  
Keyword(s):  
Gene Expression ◽  
Agrobacterium Rhizogenes ◽  
Efficient Production ◽  
Transformed Roots ◽  
Composite Plants

scholarly journals Genetically transformed hairy roots producing agropine induced on Trigonella foenum-graecum L. plant by Agrobacterium rhizogenes 1601.

2013 ◽  
Vol 7 (1) ◽  
pp. 91-98
Author(s):  
M. M. Al-Mahdawe ◽  
M. K. Al-Mallah ◽  
A. O. Al-Attrakchii
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Roots ◽  
Root Formation ◽  
Paper Electrophoresis ◽  
Transformed Roots ◽  
Fresh Weight ◽  
Trigonella Foenum Graecum ◽  
Transformed Hairy Roots ◽  
Long Time ◽  
Trigonella Foenum Graecum L

Inoculation of leaves and stems explants excised from field-grown Trigonella foenum-graecum L. with engineered Agrobacterium rhizogenes 1601 inoculum of optical density 1.90, 2.06 and 1.96 led to the formation of hairy roots on these explants. The highest percent of infection with inoculum of O.D 1.90 was 20% in leaves, and was 53.3% in stems using inoculum of O.D 2.06. Infection percent was 50% in hypocotyl compared with non-inoculated explants. The results showed that inoculation of seedlings lacking roots were slow in hairy root formation and take long time compared with their formation in intact seedlings inoculated with the same inocula. Results of paper electrophoresis of these root proved the incidence of their genetic transformation. Moreover, these transformed roots grow happily in liquid WP medium with an average fresh weight 5.9g after four weeks of culture, whereas fresh weight of normal roots was 1.02g under the same conditions.

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