Further insight concerning the TL region of the Ri plasmid of Agrobacterium rhizogenes strain A4: Transfer of a 1.9 kb fragment is sufficient to induce transformed roots on tobacco leaf fragments

1987 ◽  
Vol 210 (1) ◽  
pp. 111-115 ◽  
Author(s):  
Françoise Vilaine ◽  
Constance Charbonnier ◽  
Francine Casse-Delbart
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Transformed Roots ◽  
Tobacco Leaf ◽  
Ri Plasmid

A reliable and efficient protocol for induction of hairy roots in Agastache foeniculum

Biologia ◽  
2014 ◽  
Vol 69 (7) ◽  
Author(s):  
Elnaz Nourozi ◽  
Bahman Hosseini ◽  
Abbas Hassani
Keyword(s):  
Salicylic Acid ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
High Performance ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Biochemical Properties ◽  
Separate Experiment ◽  
Transformed Roots

AbstractHairy root culture system is a valuable tool to study the characteristics of gene expression, gene function, root biology, biochemical properties and biosynthesis pathways of secondary metabolites. In the present study, hairy roots were established in Anise hyssop (Agastache foeniculum) via Agrobacterium rhizogenes. Three strains of Agrobacterium rhizogenes (A4, A7 and 9435), were used for induction of hairy roots in four various explants (hypocotyl, cotyledon, one-month-old leaf and five-month-old leaf) of Anise hyssop. The highest frequency of transformation was achieved using A4 strain in one-month-old leaves (51.1%). The transgenic states of hairy root lines were confirmed by PCR (Polymerase chain reaction) method. High performance liquid chromatography analysis revealed that the production of rosmarinic acid (RA) in transformed roots of A. foeniculum was almost 4-fold higher than that of the non-transformed roots. In a separate experiment, hairy roots obtained from one-month-old leaves inoculated with A4 strain, were grown in liquid medium and the effects of different concentrations of salicylic acid (0.0, 0.01, 0.1 and 1 mM) and chitosan (0, 50, 100 and 150 mg L−1) (as elicitor) and sucrose (20, 30, 40 and 50 g L−1) on the growth of hairy roots were evaluated. The results showed that, 30 g L−1 sucrose and 100 mg L−1 chitosan increased the biomass of hairy root cultures and application of salicylic acid reduced the growth of hairy roots compared with control roots.

scholarly journals Transgenic root cultures of Gentiana punctata L.

2014 ◽  
Vol 68 (4) ◽  
pp. 275-280 ◽  
Author(s):  
Branka Vinterhalter ◽  
Vladimir Orbović ◽  
Dragan Vinterhalter
Keyword(s):  
Growth Rate ◽  
Agrobacterium Rhizogenes ◽  
Basal Medium ◽  
Shoot Cultures ◽  
Root Cultures ◽  
Transformed Roots ◽  
Flower Buds ◽  
Transgenic Root ◽  
Short Internodes

Shoot cultures of <em>Gentiana punctata</em> L. were inoculated with suspension of <em>Agrobacterium rhizogenes</em> strain A4 M70GUS. Hairy roots which appeared 2-3 weeks later were cultured on hormone-free, liquid, WPM (Lloyd and McCown 1980) basal medium for more than 5 years (60 subcultures). Growth rate of transformed roots was higher than the growth rate of nontransformed roots. Spontaneous shoot regeneration occured only in three culture vessels in subcultures No. 40 and 42. Plants had phenotype characteristics typical for <em>A. rhizogenes</em> transformed plants including: wrincled leaves, short internodes, plagiotropic roots and in general their growth rate was reduced. These plants also manifested precocious formation of flower buds without vernalization and flowering under in vitro conditions. Flowers were pale yellow, the same as in the standard phenotype.

Genome structure of Ri plasmid (3). Sequencing analysis of the vir region of pRi1724 in Japanese Agrobacterium rhizogenes

2000 ◽  
Vol 44 (1) ◽  
pp. 95-96 ◽  
Author(s):  
N. Niken Satuti ◽  
K. Moriguchi ◽  
M. Sato ◽  
M. Kataoka ◽  
Y. Maeda ◽  
...  
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Genome Structure ◽  
Sequencing Analysis ◽  
Ri Plasmid

scholarly journals Complementation of Agrobacterium tumefaciens tumor-inducing aux mutants by genes from the TR-region of the Ri plasmid of Agrobacterium rhizogenes

1986 ◽  
Vol 83 (18) ◽  
pp. 6935-6939 ◽  
Author(s):  
I. A. Offringa ◽  
L. S. Melchers ◽  
A. J. G. Regensburg-Tuink ◽  
P. Costantino ◽  
R. A. Schilperoort ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Agrobacterium Rhizogenes ◽  
Ri Plasmid

Induction of hairy roots in Arnica montana L. by Agrobacterium rhizogenes

2013 ◽  
Vol 8 (5) ◽  
pp. 470-479 ◽  
Author(s):  
Mariya Petrova ◽  
Ely Zayova ◽  
Mariana Vlahova
Keyword(s):  
Genetic Transformation ◽  
Agrobacterium Rhizogenes ◽  
Hairy Roots ◽  
Culture Media ◽  
Pcr Analysis ◽  
Transformed Roots ◽  
Fresh Weight ◽  
Transformation Techniques ◽  
Nutrient Media ◽  
Arnica Montana

AbstractThe induction of hairy roots in Arnica montana L. by Agrobacterium rhizogenes mediated system was established. The frequency of genetic transformation varied from 4.8 to 12% depended on method of infection. The cefotaxime at concentration of 200 mg/l proved to suppress effectively the growth of A. rhizogenes after co-cultivation. Among the three tested nutrient media: Murashige and Skoog (MS), Gamborg’s (B5) and Schenk and Hildebrandt (SH), MS medium was superior for growth and high biomass production of transformed roots compared to other culture media. After culturing for 40 days the fresh weight of clone T4 increased 7.6 fold over the non-transformed roots. The transfer of rol A, rol B and rol C genes into Arnica genome was confirmed by PCR analysis. Established genetic transformation techniques in A. montana efficiently provided and generated a large number of transformed roots — an excellent system for studying gene function and could be used for the production of secondary metabolites synthesized in roots.

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