Does a-linolenic acid in combination with linoleic acid influence liver metastasis and hepatic lipid peroxidation in BOP-induced pancreatic cancer in Syrian hamsters?

2000 ◽  
Vol 62 (6) ◽  
pp. 329-334 ◽  
Author(s):  
F.A. Wenger ◽  
M. Kilian ◽  
C.A. Jacobi ◽  
I. Schimke ◽  
H. Guski ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Lipid Peroxidation ◽  
Linoleic Acid ◽  
Liver Metastasis ◽  
Linolenic Acid ◽  
Syrian Hamsters ◽  
Hepatic Lipid ◽  
Hepatic Lipid Peroxidation

Influence of Octreotide on Liver Metastasis and Hepatic Lipid Peroxidation in BOP-Induced Pancreatic Cancer in Syrian Hamsters

Pancreas ◽  
2001 ◽  
Vol 23 (3) ◽  
pp. 266-272 ◽  
Author(s):  
F. A. Wenger ◽  
M. Kilian ◽  
I. Mautsch ◽  
C. A. Jacobi ◽  
A. Steiert ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Lipid Peroxidation ◽  
Liver Metastasis ◽  
Syrian Hamsters ◽  
Hepatic Lipid ◽  
Hepatic Lipid Peroxidation

Effects of celebrex and zyflo on liver metastasis and lipidperoxidation in pancreatic cancer in syrian hamsters

2001 ◽  
Vol 37 ◽  
pp. S110
Author(s):  
F.A. Wenger ◽  
M. Kilian ◽  
I. Schimke ◽  
H. Guski ◽  
C.A. Jacobi ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Liver Metastasis ◽  
Syrian Hamsters

scholarly journals Bariatric Surgery-Induced Weight Loss Reduces Hepatic Lipid Peroxidation Levels and Affects Hepatic Cytochrome P-450 Protein Content

2010 ◽  
Vol 251 (6) ◽  
pp. 1041-1048 ◽  
Author(s):  
Lauren N. Bell ◽  
Constance J. Temm ◽  
Rashmil Saxena ◽  
Raj Vuppalanchi ◽  
Philip Schauer ◽  
...  
Keyword(s):  
Bariatric Surgery ◽  
Lipid Peroxidation ◽  
Weight Loss ◽  
Protein Content ◽  
Hepatic Lipid ◽  
Hepatic Lipid Peroxidation ◽  
Cytochrome P 450 ◽  
Hepatic Cytochrome

scholarly journals 4-HNE Immunohistochemistry and Image Analysis for Detection of Lipid Peroxidation in Human Liver Samples Using Vitamin E Treatment in NAFLD as a Proof of Concept

2020 ◽  
Vol 68 (9) ◽  
pp. 635-643 ◽  
Author(s):  
Maren C. Podszun ◽  
Joon-Yong Chung ◽  
Kris Ylaya ◽  
David E. Kleiner ◽  
Stephen M. Hewitt ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Image Analysis ◽  
Vitamin E ◽  
Dynamic Range ◽  
Clinical Diagnostics ◽  
Automated Image Analysis ◽  
Antioxidant Vitamin ◽  
Alcoholic Fatty Liver ◽  
Hepatic Lipid ◽  
Hepatic Lipid Peroxidation

Lipid peroxidation is a common feature of liver diseases, especially non-alcoholic fatty liver disease (NAFLD). There are limited validated tools to study intra-hepatic lipid peroxidation, especially for small specimen. We developed a semi-quantitative, fully automated immunohistochemistry assay for the detection of 4-hydroxynoneal (4-HNE) protein adducts, a marker of lipid peroxidation, for adaptation to clinical diagnostics and research. We used Hep G2 cells treated with 4-HNE to validate specificity, sensitivity, and dynamic range of the antibody. Staining and semi-quantitative automated readout were confirmed in human needle-biopsy liver samples from subjects with NAFLD and normal liver histology. The ability to detect changes in lipid peroxidation was tested in paired liver biopsies from NAFLD subjects, obtained before and after 4 weeks of treatment with the antioxidant vitamin E (ClinicalTrials.gov NCT01792115, n=21). The cellular calibrator was linear and NAFLD patients had significantly higher levels of 4-HNE adducts compared to controls ( p=0.02). Vitamin E treatment significantly decreased 4-HNE ( p=0.0002). Our findings demonstrate that 4-HNE quantification by immunohistochemistry and automated image analysis is feasible and able to detect changes in hepatic lipid peroxidation in clinical trials. This method can be applied to archival and fresh samples and should be considered for use in assessing NAFLD histology.

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