Determination of in vivo protein synthesis in human T lymphocytes

2001 ◽  
Vol 20 (2) ◽  
pp. 181-182 ◽  
Author(s):  
A. JANUSZKIEWICZ ◽  
P. ESSÉN ◽  
M.A. McNURLAN ◽  
O. RINGDÉN ◽  
P.J. GARLICK ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
T Lymphocytes ◽  
Human T Lymphocytes ◽  
In Vivo Protein Synthesis

In vivo protein synthesis of circulating human T lymphocytes does not respond to a cortisol challenge within 24 h

2000 ◽  
Vol 44 (2) ◽  
pp. 202-209 ◽  
Author(s):  
A. Januszkiewicz ◽  
P. Essén ◽  
M. A. Mcnurlan ◽  
O. Ringdén ◽  
J. Wernerman ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
T Lymphocytes ◽  
Human T Lymphocytes ◽  
In Vivo Protein Synthesis

A combined stress hormone infusion decreases in vivo protein synthesis in human T lymphocytes in healthy volunteers

Metabolism ◽  
2001 ◽  
Vol 50 (11) ◽  
pp. 1308-1314 ◽  
Author(s):  
Anna Januszkiewicz ◽  
Pia Ess[eacute]n ◽  
Margaret A. McNurlan ◽  
Olle Ringd[eacute]n ◽  
Peter J. Garlick ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
T Lymphocytes ◽  
Healthy Volunteers ◽  
Stress Hormone ◽  
Combined Stress ◽  
Human T Lymphocytes ◽  
In Vivo Protein Synthesis

scholarly journals Response of in vivo protein synthesis in T lymphocytes and leucocytes to an endotoxin challenge in healthy volunteers

2002 ◽  
Vol 130 (2) ◽  
pp. 263-270 ◽  
Author(s):  
A. JANUSZKIEWICZ ◽  
K. LORÉ ◽  
P. ESSÉN ◽  
B. ANDERSSON ◽  
M. A. MCNURLAN ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
T Lymphocytes ◽  
Healthy Volunteers ◽  
Endotoxin Challenge ◽  
In Vivo Protein Synthesis

Determination of in vivo protein synthesis in human palatine tonsil

2005 ◽  
Vol 108 (2) ◽  
pp. 179-184 ◽  
Author(s):  
Anna JANUSZKIEWICZ ◽  
Maria KLAUDE ◽  
Karin LORÉ ◽  
Jan ANDERSSON ◽  
Olle RINGDÉN ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
T Lymphocytes ◽  
Immune Cells ◽  
Mononuclear Cells ◽  
Palatine Tonsil ◽  
Synthesis Rate ◽  
Protein Synthesis Rate ◽  
Palatine Tonsils ◽  
In Vivo Protein Synthesis

The palatine tonsils are constantly exposed to ingested or inhaled antigens which, in turn, lead to a permanent activation of tonsillar immune cells, even in a basic physiological state. The aim of the present study was to investigate if the immunological activation of the human palatine tonsil is reflected by a high metabolic activity, as determined by in vivo measurement of protein synthesis. The protein synthesis rate of the tonsil was also compared with that of the circulating T-lymphocytes, the total blood mononuclear cells and the whole population of blood leucocytes. Phenotypic characterization of immune-competent cells in tonsil tissue and blood was performed by flow cytometry. Pinch tonsil biopsies were taken after induction of anaesthesia in healthy adult patients (n=12) scheduled for ear surgery, uvulopalatopharyngoplasty or nose surgery. Protein synthesis was quantitatively determined during a 90-min period by a flooding-dose technique. The in vivo protein synthesis rate in the palatine tonsils was 22.8±5.7%/24 h (mean±S.D.), whereas protein synthesis in the circulating T-lymphocytes was 10.7±3.4%/24 h, in mononuclear cells was 10.8±2.8%/24 h and in leucocytes was 3.2±1.2%/24 h. CD3+ lymphocytes were the most abundant cell population in the tonsil. The in vivo protein synthesis rate in human tonsils was higher compared with the circulating immune cells. This high metabolic rate may reflect the permanent immunological activity present in human tonsils, although cell phenotypes and activity markers do not explain the differences.

Ricin inhibition of in vivo protein-synthesis in castor beans

1984 ◽  
Vol 36 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Suzanne M. Harley ◽  
Harry Beevers
Keyword(s):  
Protein Synthesis ◽  
In Vivo Protein Synthesis

Telomerase levels control the lifespan of human T lymphocytes

Blood ◽  
2003 ◽  
Vol 102 (3) ◽  
pp. 849-857 ◽  
Author(s):  
Alexander Röth ◽  
Hans Yssel ◽  
Jérôme Pène ◽  
Elizabeth A. Chavez ◽  
Mike Schertzer ◽  
...  
Keyword(s):  
T Cells ◽  
T Lymphocytes ◽  
Ectopic Expression ◽  
Dominant Negative ◽  
Major Influence ◽  
Telomeric Dna ◽  
Human Telomerase Reverse Transcriptase ◽  
Human T Lymphocytes ◽  
Human T Cells

Abstract The loss of telomeric DNA with each cell division contributes to the limited replicative lifespan of human T lymphocytes. Although telomerase is transiently expressed in T lymphocytes upon activation, it is insufficient to confer immortality. We have previously shown that immortalization of human CD8+ T lymphocytes can be achieved by ectopic expression of the human telomerase reverse transcriptase (hTERT) gene, which encodes for the catalytic component of the telomerase complex. To study the role of endogenous hTERT in the lifespan of human T cells, we blocked endogenous hTERT expression by ectopic expression of dominant-negative (DN) hTERT. Cells expressing DN-hTERT had a decreased lifespan and showed cytogenetic abnormalities, including chromosome ends without detectable telomeric DNA as well as chromosome fusions. These results indicate that while endogenous hTERT cannot prevent overall telomere shortening, it has a major influence on the longevity of human T cells. Furthermore, we show that up-regulation of hTERT in T cells upon activation decreases over time in culture. Long-term–cultured T cells also show a decreased expression of c-myc upon activation, resulting in less c-myc–induced transcription of hTERT. Moreover, memory T cells, which have expanded in vivo upon antigen encounter, expressed a lower level of hTERT upon activation than naive cells from the same donor. The observed inverse correlation between telomerase levels and replicative history suggests that telomerase levels in T cells are limiting and increasingly insufficient to sustain their proliferation.

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