Control analysis of the importance of phosphoglycerate enolase for metabolic fluxes in Lactococcus lactis subsp. lactis IL1403

2006 ◽  
Vol 153 (5) ◽  
pp. 346 ◽  
Author(s):  
B. Koebmann ◽  
C. Solem ◽  
P.R. Jensen
Keyword(s):  
Lactococcus Lactis ◽  
Control Analysis ◽  
Lactococcus Lactis Subsp ◽  
Metabolic Fluxes ◽  
Lactis Il1403

scholarly journals Starvation-Induced Stress Resistance in Lactococcus lactis subsp. lactis IL1403

1994 ◽  
Vol 60 (9) ◽  
pp. 3474-3478 ◽  
Author(s):  
Axel Hartke ◽  
Sandrine Bouche ◽  
Xavier Gansel ◽  
Philippe Boutibonnes ◽  
Yanick Auffray
Keyword(s):  
Lactococcus Lactis ◽  
Stress Resistance ◽  
Lactococcus Lactis Subsp ◽  
Induced Stress ◽  
Lactis Il1403

Cryotolerance and Cold Adaptation in Lactococcus lactis Subsp. lactis IL1403

Cryobiology ◽  
1995 ◽  
Vol 32 (6) ◽  
pp. 516-520 ◽  
Author(s):  
Jean-Michel Panoff ◽  
Bouachanh Thammavongs ◽  
Jean-Marie Laplace ◽  
Axel Hartke ◽  
Philippe Boutibonnes ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Cold Adaptation ◽  
Lactococcus Lactis Subsp ◽  
Lactis Il1403

Cloning and Characterization of a Novel tuf Promoter from Lactococcus lactis subsp. lactis IL1403

2009 ◽  
Vol 59 (4) ◽  
pp. 425-431 ◽  
Author(s):  
Eun Bae Kim ◽  
Da Chuan Piao ◽  
Jee Soo Son ◽  
Yun Jaie Choi
Keyword(s):  
Lactococcus Lactis ◽  
Lactococcus Lactis Subsp ◽  
Lactis Il1403

Generation and Characterization of Thymidine/d-Alanine Auxotrophic Recombinant Lactococcus lactis subsp. lactis IL1403 Expressing BmpB

2009 ◽  
Vol 61 (1) ◽  
pp. 29-36 ◽  
Author(s):  
Eun Bae Kim ◽  
Jee Soo Son ◽  
Qian Kun Zhang ◽  
Nam Kyung Lee ◽  
Sung Hee Kim ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Lactococcus Lactis Subsp ◽  
Lactis Il1403 ◽  
Recombinant Lactococcus Lactis

Induction of thermotolerance by chemical agents in Lactococcus lactis subsp. lactis IL1403

1995 ◽  
Vol 25 (1) ◽  
pp. 83-94 ◽  
Author(s):  
P. Boutibonnes ◽  
V. Bisson ◽  
B. Thammavongs ◽  
A. Hartke ◽  
J.M. Panoff ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Lactococcus Lactis Subsp ◽  
Chemical Agents ◽  
Lactis Il1403

scholarly journals Physical and genetic map of the chromosome of Lactococcus lactis subsp. lactis IL1403.

1992 ◽  
Vol 174 (21) ◽  
pp. 6752-6762 ◽  
Author(s):  
P Le Bourgeois ◽  
M Lautier ◽  
M Mata ◽  
P Ritzenthaler
Keyword(s):  
Lactococcus Lactis ◽  
Genetic Map ◽  
Lactococcus Lactis Subsp ◽  
Physical And Genetic Map ◽  
Lactis Il1403

Identification of Lyoprotectants Released from Gradient-freezing Pretreated Entocyte in Lactococcus Lactis Subsp. Lactis IL1403

2017 ◽  
Vol 17 (2) ◽  
pp. 122-134
Author(s):  
Lin Songyang ◽  
Kang Qiaozhen ◽  
Pan Dan ◽  
Liu Xin ◽  
Lu Laizheng ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Starter Culture ◽  
Pentose Phosphate ◽  
Plate Count ◽  
Phosphotransferase System ◽  
Lactococcus Lactis Subsp ◽  
Plate Count Method ◽  
Lactis Il1403 ◽  
Related Proteins

Performance of Lactococcus lactis as a starter culture in food production largely depends on the use of lyoprotectants during lyophilization. Gradient-freezing of bacterial cultures was conducted at 4°C, −20°C, and −80°C by storing the samples at each temperature for 2 h, successively. The entocytes extracted from the frozen cells were used as a lyoprotectant in the follow-up freeze-drying process of Lactococcus lactis subsp. lactis IL1403. The cell survival rate of gradient-freezing group increased 6.4-fold by bacterial plate count method. Furthermore, a proteomics and bioinformatics method was applied to elucidate the protein changes of Lactococcus lactis subsp. lactis IL1403 in response to gradient-freezing by gel-free proteomics using tandem mass tags (TMTs). The results showed that 121 stress-related proteins were significantly influenced by gradient-freezing. These proteins were involved in several metabolism pathways including ribosome metabolism, amino acid metabolism, quorum sensing, phosphotransferase system (PTS), pentose phosphate pathway, microbial metabolism in diverse environments, and nitrogen metabolism, etc. Some of these proteins especially the up-regulated proteins are potential lyoprotectants in vitro and they still need to be further investigated.

Growth, nisA Gene Expression, and In Situ Activity of Novel Lactococcus lactis subsp. cremoris Costarter Culture in Commercial Hard Cheese Production

2017 ◽  
Vol 80 (12) ◽  
pp. 2137-2146 ◽  
Author(s):  
Dimitrios Noutsopoulos ◽  
Athanasia Kakouri ◽  
Eleftheria Kartezini ◽  
Dimitrios Pappas ◽  
Efstathios Hatziloukas ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lactococcus Lactis ◽  
Starter Culture ◽  
Fold Increase ◽  
Raw Milk ◽  
Good Growth ◽  
Lactococcus Lactis Subsp ◽  
Quantitative Expression ◽  
Hard Cheese

ABSTRACT This study evaluated in situ expression of the nisA gene by an indigenous, nisin A–producing (NisA+) Lactococcus lactis subsp. cremoris raw milk genotype, represented by strain M78, in traditional Greek Graviera cheeses under real factory-scale manufacturing and ripening conditions. Cheeses were produced with added a mixed thermophilic and mesophilic commercial starter culture (CSC) or with the CSC plus strain M78 (CSC+M78). Cheeses were sampled after curd cooking (day 0), fermentation of the unsalted molds for 24 h (day 1), brining (day 7), and ripening of the brined molds (14 to 15 kg each) for 30 days in a fully controlled industrial room (16.5°C; 91% relative humidity; day 37). Total RNA was directly extracted from the cheese samples, and the expression of nisA gene was evaluated by real-time reverse transcription PCR (qRT-PCR). Agar overlay and well diffusion bioassays were correspondingly used for in situ detection of the M78 NisA+ colonies in the cheese agar plates and antilisterial activity in whole-cheese slurry samples, respectively. Agar overlay assays showed good growth (>8 log CFU/g of cheese) of the NisA+ strain M78 in coculture with the CSC and vice versa. The nisA expression was detected in CSC+M78 cheese samples only, with its expression levels being the highest (16-fold increase compared with those of the control gene) on day 1, followed by significant reduction on day 7 and almost negligible expression on day 37. Based on the results, certain intrinsic and mainly implicit hurdle factors appeared to reduce growth prevalence rates and decrease nisA gene expression, as well as the nisin A–mediated antilisterial activities of the NisA+ strain M78 postfermentation. To our knowledge, this is the first report on quantitative expression of the nisA gene in a Greek cooked hard cheese during commercial manufacturing and ripening conditions by using a novel, rarely isolated, indigenous NisA+ L. lactis subsp. cremoris genotype as costarter culture.

Location and characterization of aminopeptidase N in Lactococcus lactis subsp. cremoris HP

1992 ◽  
Vol 37 (1) ◽  
pp. 46-54 ◽  
Author(s):  
Fred A. Exterkate ◽  
Marian de Jong ◽  
Gerrie J. C. M. de Veer ◽  
Ronald Baankreis
Keyword(s):  
Lactococcus Lactis ◽  
Aminopeptidase N ◽  
Lactococcus Lactis Subsp
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