3D printing technologies for in vitro vaccine testing platforms and vaccine delivery systems against infectious diseases

2021 ◽  
Author(s):  
Ji Young Choi ◽  
Bhushan Mahadik ◽  
John P. Fisher
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Vaccine Delivery ◽  
Viral Disease ◽  
Delivery Systems ◽  
Vaccine Research ◽  
Vitro Model ◽  
Organ Models ◽  
Vaccine Testing

Abstract Recent advances in 3D printing (3DP) and tissue engineering approaches enable the potential application of these technologies to vaccine research. Reconstituting the native tissue or cellular microenvironment will be vital for successful evaluation of pathogenicity of viral infection and screening of potential vaccines. Therefore, establishing a reliable in vitro model to study the vaccine efficiency or delivery of viral disease is important. Here, this review summarizes two major ways that tissue engineering and 3DP strategies could contribute to vaccine research: (1) 3D human tissue models to study the response to virus can be served as a testbed for new potential therapeutics. Using 3D tissue platform attempts to explore alternative options to pre-clinical animal research for evaluating vaccine candidates. (2) 3DP technologies can be applied to improve the vaccination strategies which could replace existing vaccine delivery. Controlled antigen release using carriers that are generated with biodegradable biomaterials can further enhance the efficient development of immunity as well as combination of multiple-dose vaccines into a single injection. This mini review discusses the up-to-date report of current 3D tissue/organ models for potential vaccine potency and known bioengineered vaccine delivery systems.

scholarly journals Three-dimensionally printed polycaprolactone/multicomponent bioactive glass scaffolds for potential application in bone tissue engineering

2020 ◽  
Vol 6 (1) ◽  
pp. 57-69
Author(s):  
Amirhosein Fathi ◽  
Farzad Kermani ◽  
Aliasghar Behnamghader ◽  
Sara Banijamali ◽  
Masoud Mozafari ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Layer By Layer ◽  
Composite Scaffolds ◽  
3D Printed ◽  
Co Doped

AbstractOver the last years, three-dimensional (3D) printing has been successfully applied to produce suitable substitutes for treating bone defects. In this work, 3D printed composite scaffolds of polycaprolactone (PCL) and strontium (Sr)- and cobalt (Co)-doped multi-component melt-derived bioactive glasses (BGs) were prepared for bone tissue engineering strategies. For this purpose, 30% of as-prepared BG particles (size <38 μm) were incorporated into PCL, and then the obtained composite mix was introduced into a 3D printing machine to fabricate layer-by-layer porous structures with the size of 12 × 12 × 2 mm3.The scaffolds were fully characterized through a series of physico-chemical and biological assays. Adding the BGs to PCL led to an improvement in the compressive strength of the fabricated scaffolds and increased their hydrophilicity. Furthermore, the PCL/BG scaffolds showed apatite-forming ability (i.e., bioactivity behavior) after being immersed in simulated body fluid (SBF). The in vitro cellular examinations revealed the cytocompatibility of the scaffolds and confirmed them as suitable substrates for the adhesion and proliferation of MG-63 osteosarcoma cells. In conclusion, 3D printed composite scaffolds made of PCL and Sr- and Co-doped BGs might be potentially-beneficial bone replacements, and the achieved results motivate further research on these materials.

Fabrication and characterization of customized tubular scaffolds for tracheal tissue engineering by using solvent based 3D printing on predefined template

2021 ◽  
Vol 27 (2) ◽  
pp. 421-428
Author(s):  
Rudranarayan Kandi ◽  
Pulak Mohan Pandey ◽  
Misba Majood ◽  
Sujata Mohanty
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
3D Printing ◽  
Chemical Properties ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
In Vitro Cytotoxicity ◽  
Content Type ◽  
Tracheal Tissue

Purpose This paper aims to discuss the successful fabrication of customized tubular scaffolds for tracheal tissue engineering with a novel route using solvent-based extrusion 3D printing. Design/methodology/approach The manufacturing approach involved extrusion of polymeric ink over a rotating predefined pattern to construct customized tubular structure of polycaprolactone (PCL) and polyurethane (PU). Dimensional deviation in thickness of scaffolds were calculated for various layer thicknesses of 3D printing. Physical and chemical properties of scaffolds were investigated by scanning electron microscope (SEM), contact angle measurement, Fourier Transform Infrared Spectroscopy (FTIR) and X-ray diffraction (XRD). Mechanical characterizations were performed, and the results were compared to the reported properties of human native trachea from previous reports. Additionally, in vitro cytotoxicity of the fabricated scaffolds was studied in terms of cell proliferation, cell adhesion and hemagglutination assay. Findings The developed fabrication route was flexible and accurate by printing customized tubular scaffolds of various scales. Physiochemical results showed good miscibility of PCL/PU blend, and decrease in crystalline nature of blend with the addition of PU. Preliminary mechanical assessments illustrated comparable mechanical properties with the native human trachea. Longitudinal compression test reported outstanding strength and flexibility to maintain an unobstructed lumen, necessary for the patency. Furthermore, the scaffolds were found to be biocompatible to promote cell adhesion and proliferation from the in vitro cytotoxicity results. Practical implications The attempt can potentially meet the demand for flexible tubular scaffolds that ease the concerns such as availability of suitable organ donors. Originality/value 3D printing over accurate predefined templates to fabricate customized grafts gives novelty to the present method. Various customized scaffolds were compared with conventional cylindrical scaffold in terms of flexibility.

Use of an in vitro model in tissue engineering to study wound repair and differentiation of blastema tissue from rabbit pinna

2015 ◽  
Vol 51 (7) ◽  
pp. 680-689 ◽  
Author(s):  
Mohammad Reza Hashemzadeh ◽  
Nasser Mahdavi-Shahri ◽  
Ahmad Reza Bahrami ◽  
Masoumeh Kheirabadi ◽  
Fatemeh Naseri ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Repair ◽  
In Vitro Model ◽  
Vitro Model

scholarly journals Microcarriers Based on Glycosaminoglycan-Like Marine Exopolysaccharide for TGF-β1 Long-Term Protection

Marine Drugs ◽  
2019 ◽  
Vol 17 (1) ◽  
pp. 65 ◽  
Author(s):  
Agata Zykwinska ◽  
Mélanie Marquis ◽  
Mathilde Godin ◽  
Laëtitia Marchand ◽  
Corinne Sinquin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Target Location ◽  
Cartilage Regeneration ◽  
Transforming Growth Factor Β1 ◽  
Hydrogel Matrix ◽  
Vitro Model ◽  
Tgf Β1

Articular cartilage is an avascular, non-innervated connective tissue with limited ability to regenerate. Articular degenerative processes arising from trauma, inflammation or due to aging are thus irreversible and may induce the loss of the joint function. To repair cartilaginous defects, tissue engineering approaches are under intense development. Association of cells and signalling proteins, such as growth factors, with biocompatible hydrogel matrix may lead to the regeneration of the healthy tissue. One current strategy to enhance both growth factor bioactivity and bioavailability is based on the delivery of these signalling proteins in microcarriers. In this context, the aim of the present study was to develop microcarriers by encapsulating Transforming Growth Factor-β1 (TGF-β1) into microparticles based on marine exopolysaccharide (EPS), namely GY785 EPS, for further applications in cartilage engineering. Using a capillary microfluidic approach, two microcarriers were prepared. The growth factor was either encapsulated directly within the microparticles based on slightly sulphated derivative or complexed firstly with the highly sulphated derivative before being incorporated within the microparticles. TGF-β1 release, studied under in vitro model conditions, revealed that the majority of the growth factor was retained inside the microparticles. Bioactivity of released TGF-β1 was particularly enhanced in the presence of highly sulphated derivative. It comes out from this study that GY785 EPS based microcarriers may constitute TGF-β1 reservoirs spatially retaining the growth factor for a variety of tissue engineering applications and in particular cartilage regeneration, where the growth factor needs to remain in the target location long enough to induce robust regenerative responses.

Cytokine regulation of mucosal responses: a rational basis for new vaccine delivery strategies

1994 ◽  
Vol 6 (3) ◽  
pp. 381 ◽  
Author(s):  
AJ Husband ◽  
S Bao ◽  
W Muir ◽  
AJ Ramsay ◽  
IA Ramshaw
Keyword(s):  
Gene Therapy ◽  
Vaccine Delivery ◽  
Delivery Systems ◽  
Immune Network ◽  
Rational Basis ◽  
Cytokine Regulation ◽  
Host Environment ◽  
Delivery Strategies

In this review, cytokine regulation of mucosal responses is discussed in relation to the mucosal immune network and regulation of IgA responses. Based on this understanding, aspects of gene therapy for manipulation of the host environment and vaccine delivery systems are discussed. Although evidence obtained in vitro is briefly reviewed the general focus of this article is on evidence obtained from models in vivo.

scholarly journals Chitosan as Functional Biomaterial for Designing Delivery Systems in Cardiac Therapies

Gels ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 253
Author(s):  
Bhaumik Patel ◽  
Ravi Manne ◽  
Devang B. Patel ◽  
Shashank Gorityala ◽  
Arunkumar Palaniappan ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Cardiac Tissue ◽  
Mechanical Stability ◽  
Cardiac Tissue Engineering ◽  
Delivery Systems ◽  
Pharmaceutical Industries

Cardiovascular diseases are a leading cause of mortality across the globe, and transplant surgeries are not always successful since it is not always possible to replace most of the damaged heart tissues, for example in myocardial infarction. Chitosan, a natural polysaccharide, is an important biomaterial for many biomedical and pharmaceutical industries. Based on the origin, degree of deacetylation, structure, and biological functions, chitosan has emerged for vital tissue engineering applications. Recent studies reported that chitosan coupled with innovative technologies helped to load or deliver drugs or stem cells to repair the damaged heart tissue not just in a myocardial infarction but even in other cardiac therapies. Herein, we outlined the latest advances in cardiac tissue engineering mediated by chitosan overcoming the barriers in cardiac diseases. We reviewed in vitro and in vivo data reported dealing with drug delivery systems, scaffolds, or carriers fabricated using chitosan for stem cell therapy essential in cardiac tissue engineering. This comprehensive review also summarizes the properties of chitosan as a biomaterial substrate having sufficient mechanical stability that can stimulate the native collagen fibril structure for differentiating pluripotent stem cells and mesenchymal stem cells into cardiomyocytes for cardiac tissue engineering.

scholarly journals The Human Microbial Metabolism of Quercetin in Different Formulations: An In Vitro Evaluation

Foods ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1121 ◽  
Author(s):  
Giuseppe Di Pede ◽  
Letizia Bresciani ◽  
Luca Calani ◽  
Giovanna Petrangolini ◽  
Antonella Riva ◽  
...  
Keyword(s):  
High Performance ◽  
Microbial Metabolism ◽  
Fecal Microbiota ◽  
Delivery Systems ◽  
Dependent Manner ◽  
Main Compound ◽  
Benzoic Acid Derivatives ◽  
Vitro Model

Quercetin is one of the main dietary flavonols, but its beneficial properties in disease prevention may be limited due to its scarce bioavailability. For this purpose, delivery systems have been designed to enhance both stability and bioavailability of bioactive compounds. This study aimed at investigating the human microbial metabolism of quercetin derived from unformulated and phytosome-formulated quercetin through an in vitro model. Both ingredients were firstly characterized for their profile in native (poly)phenols, and then fermented with human fecal microbiota for 24 h. Quantification of microbial metabolites was performed by ultra-high performance liquid chromatography coupled to mass spectrometry (uHPLC-MSn) analyses. Native quercetin, the main compound in both products, appeared less prone to microbial degradation in the phytosome-formulated version compared to the unformulated one during fecal incubation. Quercetin of both products was bioaccessible to colonic microbiota, resulting in the production of phenylpropanoic acid, phenylacetic acid and benzoic acid derivatives. The extent of the microbial metabolism of quercetin was higher in the unformulated ingredient, in a time-dependent manner. This study opened new perspectives to investigate the role of delivery systems on influencing the microbial metabolism of flavonols in the colonic environment, a pivotal step in the presumed bioactivity associated to their intake.

Design and in vitro evaluation of electrospun shape memory polyurethanes for self-fitting tissue engineering grafts and drug delivery systems

2020 ◽  
Vol 110 ◽  
pp. 110675 ◽  
Author(s):  
Monika Bil ◽  
Ewa Kijeńska-Gawrońska ◽  
Eliza Głodkowska-Mrówka ◽  
Aneta Manda-Handzlik ◽  
Piotr Mrówka
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Shape Memory ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
In Vitro Evaluation

A Biological 3D Printer for the Preparation of Tissue Engineering Micro-Channel Scaffold

2015 ◽  
Vol 645-646 ◽  
pp. 1290-1297 ◽  
Author(s):  
Ya Nan Zhang ◽  
Yuan Yuan Liu ◽  
Yu Li ◽  
Shuai Li ◽  
Qing Xi Hu
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Laser Scanning ◽  
Inner Core ◽  
Vascular Supply ◽  
Hollow Fibers ◽  
Dead Cell ◽  
Layer By Layer ◽  
3D Scaffold

The clinical applications of tissue engineering are still limited by the lack of a functional vascular supply in tissue-engineered constructs. In order to improve the pre-vascularization of tissue-engineered scaffold during in vitro culture, in this study, based on three-dimensional (3D) printing technology, using the crosslinking effect of coaxial fluids (sodium alginate and CaCl2) to prepare vessel-like hollow gel fibers, then layer by layer overlapping into 3D scaffold. The biological 3D printing platform was successfully developed and a coaxial nozzle module was introduced to generate a CaCl2-in-Alginate coaxial microfluidic. The inner core diameters of the prepared hollow gel fibers were 220~380 micrometers. In addition, the influence of materials concentration and dispensing rates on hollow fiber dimension were investigated, the cell-encapsulated in the printed hollow fibers was realized and the viability of endothelial cells (ECs) was studied with Laser scanning confocal microscopy (LSCM) and Live-Dead cell staining. The 3D scaffold built by hollow fibers could improve the phenomenon of diffusion constrain and enhance the survival rate of those ECs growing at a greater depth in the construct. This study provides a new theoretical basis for the vascularization of bone scaffold.

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