Flow-mediated dilatation following wrist and upper arm occlusion in humans: the contribution of nitric oxide

2001 ◽  
Vol 101 (6) ◽  
pp. 629 ◽  
Author(s):  
Sagar N. DOSHI ◽  
Katerina K. NAKA ◽  
Nicola PAYNE ◽  
Christopher J. H. JONES ◽  
Moira ASHTON ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Upper Arm ◽  
Flow Mediated Dilatation

Flow-mediated dilatation following wrist and upper arm occlusion in humans: the contribution of nitric oxide

2001 ◽  
Vol 101 (6) ◽  
pp. 629-635 ◽  
Author(s):  
Sagar N. DOSHI ◽  
Katerina K. NAKA ◽  
Nicola PAYNE ◽  
Christopher J.H. JONES ◽  
Moira ASHTON ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Function ◽  
Brachial Artery ◽  
Peak Flow ◽  
Ultrasound Probe ◽  
Upper Arm ◽  
Flow Mediated Dilatation ◽  
Synthase Inhibitor ◽  
Increased Blood Flow ◽  
Similar Peak

Flow-mediated dilatation (FMD) of the brachial artery assessed by high-resolution ultrasound is widely used to measure endothelial function. However, the technique is not standardized, with different groups using occlusion of either the wrist or the upper arm to induce increased blood flow. The validity of the test as a marker of endothelial function rests on the assumption that the dilatation observed is endothelium-dependent and mediated by nitric oxide (NO). We sought to compare the NO component of brachial artery dilatation observed following wrist or upper arm occlusion. Dilatation was assessed before and during intra-arterial infusion of the NO synthase inhibitor NG-monomethyl-l-arginine (l-NMMA) following occlusion of (i) the wrist (distal to ultrasound probe) and (ii) the upper arm (proximal to ultrasound probe) for 5min in ten healthy males. Dilatation was significantly greater after upper arm occlusion (upper arm, 11.62±3.17%; wrist, 7.25±2.49%; P = 0.003). During l-NMMA infusion, dilatation after wrist occlusion was abolished (from 7.25±2.49% to 0.16±2.24%; P < 0.001), whereas dilatation after upper arm occlusion was only partially attenuated (from 11.62±3.17% to 7.51±2.34%; P = 0.006). The peak flow stimulus was similar after wrist and upper arm occlusion. We conclude that dilatation following upper arm occlusion is greater than that observed after wrist occlusion, despite a similar peak flow stimulus. l-NMMA infusion revealed that FMD following wrist occlusion is mediated exclusively by NO, while dilatation following upper arm occlusion comprises a substantial component not mediated by NO, most probably related to tissue ischaemia around the brachial artery. FMD following wrist occlusion may be a more valid marker of endothelial function than dilatation following upper arm occlusion.

Flow-mediated dilatation revisited

2005 ◽  
Vol 99 (4) ◽  
pp. 1623-1623 ◽  
Author(s):  
Robinson Joannides ◽  
Jeremy Bellien
Keyword(s):  
Nitric Oxide ◽  
Endothelial Function ◽  
Flow Mediated Dilation ◽  
Flow Mediated Dilatation ◽  
September Issue

This letter is in response to the Point:Counterpoint series “Flow-mediated dilation does/does not reflect nitric oxide-mediated endothelial function” that appeared in the September issue (vol. 99: 1233–1238, 2005; doi:10.1152/japplphysiol.00601.2005; http://jap.physiology.org/content/vol99/issue3/2005 ).

The impact of different supervised exercise regimens on endothelial function in patients with intermittent claudication

Vascular ◽  
2014 ◽  
Vol 23 (6) ◽  
pp. 561-569 ◽  
Author(s):  
Christopher L Delaney ◽  
Michelle D Miller ◽  
Richard B Allan ◽  
J Ian Spark
Keyword(s):  
Nitric Oxide ◽  
Exercise Training ◽  
Endothelial Function ◽  
Walking Distance ◽  
Reactive Hyperaemia ◽  
Supervised Exercise ◽  
Flow Mediated Dilatation ◽  
Group 2 ◽  
The Impact ◽  
Group 1

Background and objectives The impact of supervised exercise training on endothelial function in patients with intermittent claudication is unclear. This study assesses the impact of treadmill-based supervised exercise training alone or in combination with resistance training on pain free walking distance, flow-mediated dilatation, reactive hyperaemia index, nitric oxide and asymmetric dimethylarginine. Methods Thirty-five patients with intermittent claudication were randomised to 12 weeks of treadmill-only supervised exercise training (Group 1) or a combination of treadmill and lower-limb resistance supervised exercise training (Group 2). Pain free walking distance was assessed by six-minute walk test. Endothelial function was assessed by brachial artery flow-mediated dilatation, reactive hyperaemia index and serum analysis of asymmetric dimethylarginine and nitric oxide. Results Pain free walking distance improved within Group 1 (160 m to 204 m, p = 0.03) but not Group 2 (181 m to 188 m, p = 0.82), no between group difference. No significant change in flow-mediated dilatation or reactive hyperaemia index in either group. Nitric oxide decreased in Group 1 (15.0 µmol/L to 8.3 µmol/L, p = 0.003) but not Group 2 (11.2 µmol/L to 9.1 µmol/L, p = 0.14), p = 0.07 between groups. Asymmetric dimethylarginine decreased in Group 2 (0.61 µmol/L to 0.56 µmol/L, p = 0.03) but not Group 1 (0.58 µmol/l to 0.58 µmol/L, p = 0.776), no between group difference. Conclusion Supervised exercise training does not improve endothelial function as measured by flow-mediated dilatation, reactive hyperaemia index and nitric oxide bioavailability.

Low concentrations of adropin are associated with endothelial dysfunction as assessed by flow-mediated dilatation in patients with metabolic syndrome

2017 ◽  
Vol 55 (1) ◽  
pp. 139-144 ◽  
Author(s):  
Coskun U. Oruc ◽  
Yunus E. Akpinar ◽  
Elmire Dervisoglu ◽  
Shirkhan Amikishiyev ◽  
Artur Salmaslıoglu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Metabolic Syndrome ◽  
Endothelial Dysfunction ◽  
Cardiovascular Health ◽  
Clinical Symptoms ◽  
Close Association ◽  
Low Concentrations ◽  
Flow Mediated Dilatation ◽  
Student’S T ◽  
Endothelial Nitric Oxide

Abstract Background: In individuals with atherosclerotic risk factors, endothelial dysfunction (ED) appears as an early phase in the development of clinical symptoms. Recent studies indicate that adropin, a newly identified peptide, participates in cardiovascular health through the regulation of several metabolic events including angiogenesis and blood flow. In this study, we aimed to determine the relation of adropin with biochemical and radiologic parameters which reflect ED such as endothelial nitric oxide synthase (eNOS), endothelin 1 (ET-1), nitric oxide (NO) and flow-mediated dilatation (FMD) along with the routine biochemical measurements in patients recently diagnosed with metabolic syndrome (MetS). Methods: Fasting blood samples from 110 patients with MetS diagnosed according to the NCEP ATP III-2005 criteria were collected to measure the concentrations of adropin and other parameters including the lipid profile, insulin and glucose. Serum NOx concentrations were determined by measuring NO2 plus NO3. FMD test was performed by ultrasonography, and patients were stratified as FMD (+) or (–). Data were compared between these two subgroups and also with matching healthy controls (n=50). Biochemical data were evaluated using Student’s t or Mann-Whitney U tests. Results: Fifty-nine subjects had ED (+) and the remaining 101 subjects were ED (–). In the first group, adropin levels were significantly lower than the latter (2.13±1.05 vs. 3.41±1.63 ng/mL, respectively; p<0.001) and independently associated with FMD positivity as assessed by the logistic regression analysis. Conclusions: Low adropin level in circulation is related to ED and has a close association with FMD. Any alterations in its level may be of help in order to assess the development of ED before the occurrence of clinical symptoms in patients with metabolic syndrome.

scholarly journals Flow-mediated dilatation in the superficial femoral artery is nitric oxide mediated in humans

2008 ◽  
Vol 586 (4) ◽  
pp. 1137-1145 ◽  
Author(s):  
M. Kooijman ◽  
D. H. J. Thijssen ◽  
P. C. E. De Groot ◽  
M. W. P. Bleeker ◽  
H. J. M. Van Kuppevelt ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Femoral Artery ◽  
Superficial Femoral Artery ◽  
Flow Mediated Dilatation

scholarly journals Polycystin deficiency induces dopamine-reversible alterations in flow-mediated dilatation and vascular nitric oxide release in humans

2015 ◽  
Vol 87 (2) ◽  
pp. 465-472 ◽  
Author(s):  
Aurélien Lorthioir ◽  
Robinson Joannidès ◽  
Isabelle Rémy-Jouet ◽  
Caroline Fréguin-Bouilland ◽  
Michèle Iacob ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Release ◽  
Flow Mediated Dilatation

scholarly journals Acute reductions in haematocrit increase flow‐mediated dilatation independent of resting nitric oxide bioavailability in humans

2020 ◽  
Vol 598 (19) ◽  
pp. 4225-4236 ◽  
Author(s):  
Ryan L. Hoiland ◽  
Joshua C. Tremblay ◽  
Benjamin S. Stacey ◽  
Geoff B. Coombs ◽  
Daniela Nowak‐Flück ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Flow Mediated Dilatation ◽  
Nitric Oxide Bioavailability
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