Diminished skin blood flow in Type I diabetes: evidence for non-endothelium-dependent dysfunction

2001 ◽  
Vol 101 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Abram KATZ ◽  
Karin EKBERG ◽  
Bo-Lennart JOHANSSON ◽  
John WAHREN
Keyword(s):  
Blood Flow ◽  
Sodium Nitroprusside ◽  
Skin Blood Flow ◽  
Perfusion Imaging ◽  
Healthy Subjects ◽  
Type I Diabetes ◽  
Diabetic Patients ◽  
Type I ◽  
Laser Doppler Perfusion Imaging ◽  
Before And After

The purpose of this study was to quantify the extent to which skin blood flow (SBF) responses to application of endothelium-dependent and -independent vasodilating agents differ between Type I diabetic patients and healthy subjects. Patients and matched controls were studied after an overnight fast. SBF was determined with laser Doppler perfusion imaging before and after iontophoresis of acetylcholine (Ach; endothelium-dependent) and sodium nitroprusside (SNP; endothelium-independent). Basal SBF did not differ significantly between groups. Iontophoresis of ACh and SNP increased SBF 20-fold in controls. In the patients, the increases in SBF following iontophoresis of ACh and SNP were reduced by 18% and 19%, respectively, versus controls (P < 0.05 for both). These data demonstrate that Type I diabetic patients have similar diminished SBF responses to iontophoresis of ACh and SNP, which suggests that non-endothelial-dependent factors are primarily responsible for the diminished SBF responses.

Skin Microcirculation in Patients with Type I Diabetes with and without Neuropathy after Neurovascular Stimulation

1998 ◽  
Vol 94 (3) ◽  
pp. 255-261 ◽  
Author(s):  
Thomas Forst ◽  
Andreas Pfützner ◽  
Thomas Kunt ◽  
Thomas Pohlmann ◽  
Ulrike Schenk ◽  
...  
Keyword(s):  
Blood Flow ◽  
Diabetic Neuropathy ◽  
Type I Diabetes ◽  
Capillary Blood ◽  
Capillary Blood Flow ◽  
Diabetic Patients ◽  
Type I ◽  
Skin Microcirculation ◽  
Skin Perfusion ◽  
Diabetes Patients

1. Neurovascular inflammation is impaired in patients suffering from diabetic neuropathy. The aim of our study was to evaluate the distribution of nutritive and total skin blood flow in diabetic patients with and without neuropathy after neurovascular stimulation with acetylcholine. 2. Twenty patients with Type I diabetes, 10 with and 10 without neuropathy, and 10 age-matched non-diabetic control subjects, underwent microvascular investigations before and after neurovascular stimulation by intracutaneous application of acetylcholine. The capillary blood cell velocity in the nailfold of the hallux was measured by videophotometric capillaroscopy, and the total skin microcirculation in the same area by laser Doppler flowmetry. 3. The increase in total skin blood flow was significantly impaired in the group of neuropathic diabetic patients compared with the non-neuropathic diabetic patients (17.5 ± 83 versus 51.0 ± 16.2; P < 0.05) and the non-diabetic subjects (17.5 ± 8.3 versus 67.8 ± 19.7; P < 0.01). The increase in capillary blood flow was not significantly impaired in Type 1 diabetes patients with neuropathy. 4. The ratio between capillary blood flow and total skin perfusion decreased significantly in the control group (from 0.82 ± 0.15 to 0.47 ± 0.11; P < 0.005) and in the Type I diabetes patients without neuropathy (from 0.79 ± 0.12 to 0.43 ± 0.12; P < 0.05), whereas the decrease in the neuropathic group was statistically insignificant (from 1.05 ± 0.19 to 0.72 ±0.16). 5. Diminished total skin perfusion in the foot after intracutaneous stimulation with acetylcholine in Type I diabetes patients is associated with diabetic neuropathy, indicating a disturbance in the neurovascular reflex arc. This impaired neurovascular response is caused by a diminished total and sub-papillary blood flow and not by a diminished nutritive capillary flow. There is no evidence of a diminished nutritive capillary blood flow during neurogenic inflammation in Type I diabetes patients suffering from diabetic neuropathy.

Laser Doppler Perfusion Imaging: A New Technique for Measuring Skin Blood Flow in Rheumatology

Rheumatology ◽  
1993 ◽  
Vol 32 (10) ◽  
pp. 938-938 ◽  
Author(s):  
A. M. SEIFALIAN ◽  
K. HOWELL ◽  
G. STANSBY ◽  
A. E. JACKSON ◽  
G. HAMILTON ◽  
...  
Keyword(s):  
Blood Flow ◽  
Skin Blood Flow ◽  
Perfusion Imaging ◽  
Laser Doppler ◽  
New Technique ◽  
Laser Doppler Perfusion Imaging ◽  
A New Technique

scholarly journals Laser Doppler Perfusion Imaging Is Useful in the Study of Raynaud’s Phenomenon and Improves the Capillaroscopic Diagnosis

2009 ◽  
Vol 36 (10) ◽  
pp. 2257-2263 ◽  
Author(s):  
EDOARDO ROSATO ◽  
FEDERICA BORGHESE ◽  
SIMONETTA PISARRI ◽  
FELICE SALSANO
Keyword(s):  
Blood Flow ◽  
Distribution Pattern ◽  
Skin Blood Flow ◽  
Perfusion Imaging ◽  
Raynaud’S Phenomenon ◽  
Raynaud's Phenomenon ◽  
Laser Doppler ◽  
Healthy Controls ◽  
Laser Doppler Perfusion Imaging ◽  
Distal Perfusion

Objective.To investigate capillary morphology and skin blood flow of dorsal hands by nailfold videocapillaroscopy (NVC) and laser Doppler perfusion imaging (LDPI), respectively, in patients with primary Raynaud’s phenomenon (PRP) and systemic sclerosis (SSc) and to compare the results with those obtained in healthy controls.Methods.The study group consisted of 142 patients with SSc, 88 patients with PRP, and 147 healthy controls. NVC was performed in all the groups examined. In patients with SSc the capillaroscopic pattern was classified as early, active, or late group pattern. A baseline skin blood flow determination of the dorsum of the subject’s hands was acquired through a low-energy 670 nm Lisca Laser Doppler Perfusion Imager.Results.In the healthy controls the perfusion distribution pattern was homogeneous, with a proximal-distal perfusion gradient. In patients with PRP, the perfusion distribution pattern was homogeneous, but the proximal-distal perfusion gradient was absent. Finally, in patients with SSc the perfusion distribution pattern was dyshomogeneous and a proximal-distal gradient was absent. The minimum perfusion, mean perfusion, maximum perfusion, and standard deviation, calculated as variation by means of each measurement site, were significantly different in all the groups examinated.Conclusion.NVC represents the best method to analyze microvascular damage in rheumatic diseases. LDPI improves the evaluation of vascular damage in patients with SSc. The LDPI and the capillaroscopic images fully matched the definition of the various stages of vascular digital damage in SSc.

scholarly journals Laser Doppler Perfusion Imaging of Skin Blood Flow Using Red and Near-Infrared Sources

1996 ◽  
Vol 107 (6) ◽  
pp. 882-886 ◽  
Author(s):  
Neil C. Abbot ◽  
William R. Ferrell ◽  
John C. Lockhart ◽  
J. Graham Lowe
Keyword(s):  
Blood Flow ◽  
Skin Blood Flow ◽  
Perfusion Imaging ◽  
Near Infrared ◽  
Laser Doppler ◽  
Laser Doppler Perfusion Imaging ◽  
Infrared Sources

Diminished skin blood flow in Type I diabetes: evidence for non-endothelium-dependent dysfunction

2001 ◽  
Vol 101 (1) ◽  
pp. 59 ◽  
Author(s):  
Abram KATZ ◽  
Karin EKBERG ◽  
Bo-Lennart JOHANSSON ◽  
John WAHREN
Keyword(s):  
Blood Flow ◽  
Skin Blood Flow ◽  
Type I Diabetes ◽  
Type I

Stem Cell-Based Therapies: A New Ray of Hope for Diabetic Patients

2019 ◽  
Vol 14 (2) ◽  
pp. 146-151 ◽  
Author(s):  
Junaid Khan ◽  
Amit Alexander ◽  
Mukta Agrawal ◽  
Ajazuddin ◽  
Sunil Kumar Dubey ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Drug Therapy ◽  
Type I Diabetes ◽  
Embryonic Stem ◽  
Health Concern ◽  
Future Research ◽  
Diabetic Patients ◽  
Successful Implementation ◽  
Type I

Diabetes and its complications are a significant health concern throughout the globe. There are physiological differences in the mechanism of type-I and type-II diabetes and the conventional drug therapy as well as insulin administration seem to be insufficient to address the problem at large successfully. Hypoglycemic swings, frequent dose adjustments and resistance to the drug are major problems associated with drug therapy. Cellular approaches through stem cell based therapeutic interventions offer a promising solution to the problem. The need for pancreatic transplants in case of Type- I diabetes can also be by-passed/reduced due to the formation of insulin producing β cells via stem cells. Embryonic Stem Cells (ESCs) and induced Pluripotent Stem Cells (iPSCs), successfully used for generating insulin producing &#946; cells. Although many experiments have shown promising results with stem cells in vitro, their clinical testing still needs more exploration. The review attempts to bring into light the clinical studies favoring the transplantation of stem cells in diabetic patients with an objective of improving insulin secretion and improving degeneration of different tissues in response to diabetes. It also focuses on the problems associated with successful implementation of the technique and possible directions for future research.

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