Muscle composition in relation to age and sex

1991 ◽  
Vol 81 (2) ◽  
pp. 249-256 ◽  
Author(s):  
A. M. Forsberg ◽  
E. Nilsson ◽  
J. Werneman ◽  
J. Bergström ◽  
E. Hultman
Keyword(s):  
Protein Content ◽  
Dna Content ◽  
Soluble Protein ◽  
Fat Content ◽  
Age Group ◽  
Soluble Protein Content ◽  
Muscle Composition ◽  
Tissue Weight ◽  
Total Creatine ◽  
Age And Sex

1. A method is described enabling the determination of fat, water, electrolytes, protein, DNA, RNA and total creatine in a single sample of human muscle obtained by the percutaneous needle-biopsy technique. The amino acid content can also be analysed in the same muscle sample. 2. Fifty healthy subjects were studied: 29 between 19 and 40 years of age, 11 between 41 and 60 years of age, and 10 between 61 and 85 years of age. The two groups aged less than 60 years showed only marginal differences in muscle composition, whereas the highest age group showed increases in muscle fat content in relation to tissue weight and decreases in alkali-soluble protein content in relation to both tissue weight and tissue DNA content. Also, potassium, magnesium, total creatine and RNA contents were decreased in this age group when related to tissue DNA content. When alkali-soluble protein was used as a reference base, only magnesium content was decreased. 3. A comparison was also made between female (n = 23) and male (n = 18) subjects in the age groups below 60 years. Differences observed included a higher fat content in female muscle, and an increase in total creatine content in relation to tissue weight. The alkali-soluble protein content was lower per muscle cell in the females when calculated on the basis of DNA content. 4. The results show that in the assessment of muscle constituents, age and sex must be taken into account.

scholarly journals Effects of Water Particles in the Jinjiang River Estuary on the Physiological and Biochemical Characteristics of Microcystit Flos-Aquae

2021 ◽  
Author(s):  
Yiting Nan ◽  
Peiyong Guo ◽  
Hui Xing ◽  
Sijia Chen ◽  
Bo Hu ◽  
...  
Keyword(s):  
Particle Size ◽  
Particulate Matter ◽  
Protein Content ◽  
Suspended Particulate Matter ◽  
Soluble Protein ◽  
Suspended Particles ◽  
Dose Effect ◽  
Soluble Protein Content ◽  
Sod Activity ◽  
Suspended Particulate

Abstract The effects of different concentrations (100,150,200,250 mg/L) and different particle sizes (0–75µm, 75–120µm, 120–150µm, 150–500µm) on soluble protein content, SOD and CAT activity, MDA content, chlorophyll a content and photosynthetic parameters of Microcystis flos-aquae were studied, the mechanism of the effect of suspended particulate matter on the physiology and biochemistry of Microcystis flos-aquae was discussed. The results showed that the soluble protein content of Microcystis flos-aquae did not change obviously after being stressed by suspended particles of different concentration/diameter. The SOD activity of Microcystis flos-aquae increased at first and then decreased with the increase of the concentration of suspended particulate matter. The SOD activity of Microcystis flos-aquae reached 28.03 U/mL when the concentration of suspended particulate matter was 100 mg/L. The CAT activity of Microcystis flos-aquae increased with the increase of the concentration of suspended particles, and reached the maximum value of 12.45 U/mgprot in the concentration group of 250 mg/L, showing a certain dose-effect. The effect of small particle size on SOD, CAT and MDA of Microcystis flos-aquae was more significant than that of large particle size. The larger the concentration and the smaller the particle size, the stronger the attenuation of light and the lower the content of chlorophyll a. Both Fv/Fm and Fv/F0 of Microcystis flos-aquae increased at first and then decreased under different concentration/size of suspended particles. The relative electron transfer rate gradually returned to the normal level with the passage of time. There was no significant difference in α value between treatment group and control group, ETRmax and Ik decreased.

scholarly journals Mutation in neurospora crassa with leaf extract of citrus aurantifolia and their soluble protein content

1970 ◽  
Vol 19 (2) ◽  
pp. 151-155
Author(s):  
Apurba Lal Ray ◽  
Mahbuba Akhter Jahan ◽  
Tahsina Rahim
Keyword(s):  
Neurospora Crassa ◽  
Protein Content ◽  
Soluble Protein ◽  
Mycelial Growth ◽  
Leaf Extract ◽  
Mutagenic Effect ◽  
Wild Type ◽  
Soluble Protein Content ◽  
Citrus Aurantifolia ◽  
Morphological Mutants

Leaf extract of Citrus aurantifolia exhibited remarkable inhibitor effect on the radial mycelial growth of Neurospora crassa. The extract also showed mutagenic effect and atleast six morphological mutants of the fungus were detected including albino (al 243), vigorous (vg 117), fluffy (fl 220), colonial (cl 232), conidial band (con. band 171) and dirty (dir 83). The mutants were used for estimation of soluble protein in comparison with the wild type (Ema). The soluble protein content increased to some extent in case of the mutants con. band 171 (192.86 μg/ml), cl 232 (188.57 μg/ml) and vg 117 (186.43 μg/ml) as compared to the wild type (182.14 μg/ml). On the other hand, the soluble protein content was remarkably decreased in case of the mutant al 243 (94.28 μg/ml), which was about 50% less than the control. This indicates that the leaf extract not only effect colony morphology but possesses profound effect on growth and metabolism of the fungus. Key words: Neurospora crassa; Mutation; Leaf extract; Soluble protein DOI: http://dx.doi.org/10.3329/dujbs.v19i2.8958 DUJBS 2010; 19(2): 151-155

Changes in protein during development of Triturus embryos

Development ◽  
1973 ◽  
Vol 30 (3) ◽  
pp. 647-659
Author(s):  
Hiroshi Imoh ◽  
Tsutomu Minamidani
Keyword(s):  
Protein Synthesis ◽  
Protein Content ◽  
Sodium Carbonate ◽  
Dna Content ◽  
Total Protein ◽  
Soluble Protein ◽  
Nuclear Protein ◽  
Polyacrylamide Gel Electrophoresis ◽  
Tail Bud ◽  
Stage Of Development

The present paper reports basic data on DNA content, protein content, and protein synthesis in Triturus pyrrhogaster embryos during development from cleavage to the hatching stage. Except for measurements of DNA and total protein contents, embryos were labeled with sodium carbonate-14C for 10 h and fractionated into embryonic cell components, i.e. cytoplasmic mass, yolk and pigment granules, and nuclei, in a discontinuous density gradient of sucrose. The protein content and the radioactivity incorporated into protein were measured in each fraction. Those fractions combining protein soluble in buffer at pH 8·3 and in 0·25 N-HCl were further studied with polyacrylamide gel electrophoresis. In the newt embryo, four stages of active DNA increase were observed when cultured at constant temperature; they were gastrula, neurula, late tail-bud, and before-hatching stages. Total protein per embryo decreased from 3 to 2 mg during the development studied. The content of cytoplasmic soluble protein per embryo was low and constant throughout development. Synthesis of the fraction was observed at the earliest stage of development studied though the rate was not high and specific activity of the soluble protein increased during development. Qualitative changes in the newly synthesized protein were observed. With the yolk fraction, synthesis of protein, other than from probable contamination with the cytoplasmic fraction, was not detected and a detailed description was omitted. Changes were observed at two stages of development in the synthesis of nuclear protein soluble in buffer at pH 8·3, the first at gastrulation and the second at late tail-bud stage. The change at gastrulation seemed to be the start of syntheses of the nuclear soluble proteins, while quantitative enhancement rather than qualitative change was noticed at late tail-bud stage. Most of the nuclear protein soluble in 0·25 N-HCI was histone. The histone content increased in accordance with increase in the DNA content and the rate of DNA accumulation was accompanied by proportionate incorporation of radioactivity into histone. Among histone fractions, unique behaviour of the very lysine-rich histone was observed. The availability of [14C]sodium carbonate in rough estimations of protein synthesis in embryos and significance of the data obtained have been discussed.

scholarly journals Mycorrhizal efficacy of trifoliate orange seedlings on alleviating temperature stress

2011 ◽  
Vol 57 (No. 10) ◽  
pp. 459-464 ◽  
Author(s):  
Q.S. Wu
Keyword(s):  
High Temperature ◽  
Low Temperature ◽  
Protein Content ◽  
Temperature Stress ◽  
Soluble Protein ◽  
Mycorrhizal Colonization ◽  
Poncirus Trifoliata ◽  
Trifoliate Orange ◽  
Soluble Protein Content ◽  
Entry Points

Citrus plants often suffer from temperature stress, which seriously inhibits tree growth and even results in tree death. The present experiment was conducted to evaluate the effects of Glomus mosseae on growth, root morphology, superoxide dismutase (SOD) and catalase (CAT) activities, and soluble protein content of trifoliate orange (Poncirus trifoliata) seedlings at low (15°C), optimum (25°C) and high (35°C) temperatures. Sixty-eight days after temperature stresses, mycorrhizal colonization and number of both entry points and vesicles were significantly inhibited by low or high temperature. Mycorrhizal seedlings recorded significantly higher growth characteristics than non-mycorrhizal seedlings at both optimum and high temperatures, but the beneficial effects were almost lost at low temperature. Generally, mycorrhizal seedlings presented notably higher root traits (projected area, surface area, number of forks and volume) than non-mycorrhizal seedlings regardless of temperature levels. Mycorrhizal colonization significantly increased SOD and CAT activities and soluble protein content at high temperature, increased only SOD activity at optimum temperature, and decreased only soluble protein content at low temperature. It suggests that mycorrhizal alleviation of temperature stress in trifoliate orange seedlings was at high temperature, but the alleviation was obviously weakened at low temperature.

Ozone effects on soluble protein content of Acer negundo, Quercus robur and Platanus spp. pollen

Aerobiologia ◽  
2013 ◽  
Vol 29 (3) ◽  
pp. 443-447 ◽  
Author(s):  
H. Ribeiro ◽  
L. Duque ◽  
R. Sousa ◽  
I. Abreu
Keyword(s):  
Protein Content ◽  
Soluble Protein ◽  
Quercus Robur ◽  
Soluble Protein Content ◽  
Acer Negundo

Effects of auxins on soluble carbohydrates, starch and soluble protein content in Aechmea blanchetiana (Bromeliaceae) cultured in vitro

2010 ◽  
Vol 125 (3) ◽  
pp. 451-455 ◽  
Author(s):  
Edison Paulo Chu ◽  
Armando Reis Tavares ◽  
Shoey Kanashiro ◽  
Patricia Giampaoli ◽  
Erika Szeibel Yokota
Keyword(s):  
Protein Content ◽  
Soluble Protein ◽  
Soluble Carbohydrates ◽  
Soluble Protein Content

Photosynthesis, vegetative habit and culinary properties of sage (Salvia officinalis) in response to low-light conditions

2014 ◽  
Vol 94 (5) ◽  
pp. 881-889 ◽  
Author(s):  
Christine Mapes ◽  
Yan Xu
Keyword(s):  
Protein Content ◽  
Plant Height ◽  
Soluble Protein ◽  
Photochemical Efficiency ◽  
Leaf Size ◽  
Salvia Officinalis ◽  
Light Conditions ◽  
Soluble Protein Content ◽  
Low Light ◽  
Double Blind

Mapes, C. and Xu, Y. 2014. Photosynthesis, vegetative habit and culinary properties of sage (Salvia officinalis) in response to low-light conditions. Can. J. Plant Sci. 94: 881–889. Culinary sage (Salvia officinalis) is known to grow better at sunny locations with good drainage. However, when there is a necessity to propagate it in controlled environments with minimal additional lighting, or at shady locations, how significantly its growth and production would be restricted has not been well investigated. The objective of this study was to examine the responses of sage grown in a greenhouse over the winter when ambient light in the greenhouse is lower than 212 μmol m−2 s−1 (or a daily light integral of 9.2 mol m−2 d−1). Sage seedlings were planted in growing media and covered with various shade cloths to reach 70, 50 and 30% of the ambient irradiance. Photochemical efficiency and in situ chlorophyll status were estimated at 7, 21, and 56 d. The daily leaf emergence rate was calculated by monitoring changes in leaf number. Plant height, leaf size, biomass, aroma rating and soluble protein content of the sage plants grown under various light levels were measured at 84 d. The results indicate that light impacted the integrity of chloroplasts and the photosynthetic capacity of sage plants, as manifested by the lower the light level, the more the declines in variable fluorescence/maximal fluorescence and chlorophyll content index. Biochemical analysis revealed that the concentration of chlorophyll (per gram leaf dry mass) slightly increased under 50 and 30% light, but that of carotenoids was not changed. Light also affected the vegetative habit of sage plants. With reduced light intensity, plant height increased, whereas leaf size and number decreased. Double-blind aroma testing suggested that adequate light intesity might be required for the biosynthesis of essential oil in sage, attenuating its aroma. The soluble protein content in sage leaves declined at 50 and 30% light, suggesting a deminished source of essential amino acids contained in the plant. In summary, sage responds sensitively to reducing irradiance, which directly restricts its photosynthesis, and thus alters its vegetative growth and culinary properties.

scholarly journals Evaluation of a Commercial Extract of Giant Knotweed on Drought Tolerance of Impatiens

HortScience ◽  
2014 ◽  
Vol 49 (8) ◽  
pp. 1034-1040 ◽  
Author(s):  
Diana R. Cochran ◽  
Richard L. Harkess ◽  
Patricia R. Knight ◽  
Maria Tomaso-Peterson ◽  
Eugene K. Blythe ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Protein Content ◽  
Soluble Protein ◽  
Dry Weight ◽  
Soluble Protein Content ◽  
Commercial Extract ◽  
Impatiens Walleriana ◽  
Giant Knotweed ◽  
Reynoutria Sachalinensis ◽  
Polygonum Sachalinense

Regalia®, a commercial extract of giant knotweed [Fallopia sachalinensis F. Schmidt (synonyms: Reynoutria sachalinensis (F. Schmidt) Nakai, Polygonum sachalinense F. Schmidt, Tiniaria sachalinesis (F. Schmidt) Janch.)], was evaluated for its potential to enhance drought tolerance of container-grown impatiens (Impatiens walleriana Hook. f. ‘Super Elfin XP White’). In two separate experiments, Regalia® was foliar-applied once a week for 4 weeks at four different rates (0, 5, 10, or 15 mL·L−1). In Expt. 1, Regalia® was applied to impatiens grown under three target substrate volumetric water contents (TVWCs): 85%, 55%, or 25%. In Expt. 2, Regalia® was applied to impatiens watered with 1, 3, or 6 days between waterings (DBW). In Expt. 1, root dry weight (RDW) of impatiens receiving applications of Regalia® at the 0.5× rate was greater compared with the 0.0× rate across all TVWCs. Additionally, soluble protein content was greater after Regalia® application at the 0.5×, 1.0×, or 1.5× rates compared with the 0.0× rate for plants grown at 55% TVWC. In Expt. 2, leaf greenness (SPAD) and leaf net photosynthetic rate (Pn) were greater with Regalia® applied at the 0.5× and 1.0× rates compared with the 0.0× rate, respectively. Soluble protein content was greater in impatiens treated with Regalia® at the 1.5× rate and 1 DBW and the 0.5× rate with 3 DBW compared with the 0.0× rate with 1 or 3 DBW. However, there was no indication that impatiens grown under different moisture levels had increased drought tolerance after application of Regalia®.

Sign in / Sign up

Export Citation Format

Share Document