Monoclonal Antibodies to Measure Wheat Gliadin in Gluten-Free Foods

1986 ◽  
Vol 71 (s15) ◽  
pp. 69P-69P
Author(s):  
A.R. Freedman ◽  
G. Galfre ◽  
P.J. Ciclitira
Keyword(s):  
Monoclonal Antibodies ◽  
Gluten Free ◽  
Wheat Gliadin

Monoclonal antibody ELISA to quantitate wheat gliadin contamination of gluten-free foods

1987 ◽  
Vol 98 (1) ◽  
pp. 123-127 ◽  
Author(s):  
A.R. Freedman ◽  
G. Galfrè ◽  
E. Gal ◽  
H.J. Ellis ◽  
P.J. Ciclitira
Keyword(s):  
Monoclonal Antibody ◽  
Gluten Free ◽  
Antibody Elisa ◽  
Wheat Gliadin

Chromosomal control of wheat gliadin protein epitopes: analysis with specific monoclonal antibodies

1991 ◽  
Vol 82 (1) ◽  
pp. 44-53 ◽  
Author(s):  
J. H. Skerritt ◽  
O. Martinuzzi ◽  
E. V. Metakovsky
Keyword(s):  
Monoclonal Antibodies ◽  
Wheat Gliadin ◽  
Gliadin Protein

scholarly journals Evaluation of a gluten free product containing wheat gliadin in patients with coeliac disease.

BMJ ◽  
1984 ◽  
Vol 289 (6437) ◽  
pp. 83-83 ◽  
Author(s):  
P J Ciclitira ◽  
H J Ellis ◽  
N L Fagg
Keyword(s):  
Coeliac Disease ◽  
Free Product ◽  
Gluten Free ◽  
Wheat Gliadin

Detection of wheat gliadin contamination of gluten-free foods by a monoclonal antibody dot immunobinding assay

1987 ◽  
Vol 166 (2-3) ◽  
pp. 323-328 ◽  
Author(s):  
Andrew R. Freedman ◽  
Giovani Galfre ◽  
Eva Gal ◽  
H.Julia Ellis ◽  
Paul J. Ciclitira
Keyword(s):  
Monoclonal Antibody ◽  
Gluten Free ◽  
Wheat Gliadin

scholarly journals Production of monoclonal antibodies to immunogenic peptides of ω-glaidin/c-hordein and rye-secalin for gluten free food screening

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Widya Abd Wahab ◽  
H Julia Ellis ◽  
Paul J Ciclitira
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Free Food ◽  
Enzyme Linked Immunosorbent Assay ◽  
Gluten Free ◽  
Immunization Schedule ◽  
Booster Injection ◽  
Immunogenic Peptides ◽  
T Cell Lines ◽  
Class Antibody

Introduction: The ω-glaidin/c-hordein (QPFPQPEQPFPW) and rye-secalin (QPFPQPQQPIPQ) peptides have previously demonstrated immunogenicity in sensitised coeliac T cell lines. The development of monoclonal antibody to those immunogenic peptides is presented with a view of developing an improved enzyme linked immunosorbent assay (ELISA) as a reliable tool to screen the safety of foods specialised for coeliac disease (CD) patients. Methods: Balb/C mice were fed with gluten free food. The immunogens were conjugated to purified tuberculin protein derivative (PPD) with glutaraldehyde and emulsified in Freund’s adjuvant. The employed immunization schedule included 3-5 weeks intervals, followed by a final intravenous injection without adjuvant, 3-4 days prior to fusion. Results: The antibody produced was IgM rather than IgG, although the hybridoma was successfully generated. The IgM class antibody is known to be relatively unstable, so could not be used in kits designed for food screening. Conclusions: The immunogenic peptides could possibly be used to raise monoclonal antibodies for gluten screening. However, a single booster injection might be insufficient to stimulate the spleen directly or the mice should be immunized with higher concentration of immunogen. This could be improved by including multiple booster administrations and increasing the dose.

Specificity Analysis of Anti-gliadin Mouse Monoclonal Antibodies Used for Detection of Gliadin in Food for Gluten-free Diet

2007 ◽  
Vol 55 (7) ◽  
pp. 2627-2632 ◽  
Author(s):  
Daniel Sánchez ◽  
Ludmila Tučková ◽  
Martin Burkhard ◽  
Jan Plicka ◽  
Thomas Mothes ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Gluten Free Diet ◽  
Gluten Free

Ultrastructural analysis of unique glycoconjugates in the rat olfactory system

1992 ◽  
Vol 50 (1) ◽  
pp. 890-891
Author(s):  
James E. Crandall ◽  
Linda C. Hassinger ◽  
Gerald A. Schwarting
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Monoclonal Antibodies ◽  
Olfactory System ◽  
Olfactory Bulbs ◽  
Temporal And Spatial Patterns ◽  
Carbohydrate Epitopes ◽  
Olfactory Systems ◽  
Temporal And Spatial ◽  
Cell Surface Glycoconjugates

Cell surface glycoconjugates are considered to play important roles in cell-cell interactions in the developing central nervous system. We have previously described a group of monoclonal antibodies that recognize defined carbohydrate epitopes and reveal unique temporal and spatial patterns of immunoreactivity in the developing main and accessory olfactory systems in rats. Antibody CC2 reacts with complex α-galactosyl and α-fucosyl glycoproteins and glycolipids. Antibody CC1 reacts with terminal N-acetyl galactosamine residues of globoside-like glycolipids. Antibody 1B2 reacts with β-galactosyl glycolipids and glycoproteins. Our light microscopic data suggest that these antigens may be located on the surfaces of axons of the vomeronasal and olfactory nerves as well as on some of their target neurons in the main and accessory olfactory bulbs.

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