Bile Salts, Hypotension and Obstructive Jaundice

1984 ◽  
Vol 67 (2) ◽  
pp. 177-183 ◽  
Author(s):  
A. Bomzon ◽  
J. P. M. Finberg ◽  
D. Tovbin ◽  
S. G. Naidu ◽  
O. S. Better
Keyword(s):  
Smooth Muscle ◽  
Bile Duct ◽  
Obstructive Jaundice ◽  
Bile Salts ◽  
Vas Deferens ◽  
Bile Duct Ligation ◽  
Peripheral Resistance ◽  
Duct Ligation ◽  
Isolated Rat

1. We have examined the effects of bile duct ligation on vascular and extravascular smooth muscle responsiveness to noradrenaline and tyramine using isolated rat hindlimb perfusion, and portal vein and vas deferens preparations. 2. Bile duct ligation reduced the contractile responses to noradrenaline of vascular and extra-vascular smooth muscle. 3. Exposure of smooth muscle to some bile salts caused a reduction in contractility. 4. This effect was dependent upon bile salt type and concentration. 5. These studies in vitro suggest that the reduced total peripheral resistance and hypotension seen in obstructive jaundice cannot be explained by a spasmolytic effect of some of the bile salts on smooth muscle.

scholarly journals A new model of reversible obstructive jaundice using rapidly absorbable suture materials

2012 ◽  
Vol 35 (6) ◽  
pp. 351 ◽  
Author(s):  
Nurettin Kahramansoy ◽  
Hayri Erkol ◽  
Edip E Yilmaz ◽  
Mustafa Şit ◽  
Fahri Yilmaz ◽  
...  
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Obstructive Jaundice ◽  
Surgical Intervention ◽  
Bile Duct Ligation ◽  
Absorbable Suture ◽  
Suture Materials ◽  
Common Bile Duct Ligation ◽  
Duct Ligation ◽  
Morphologic Changes

Purpose: Reversible obstructive jaundice models have some limiting features, including the need for a second anaesthesia, re-laparotomy and surgical intervention after common bile duct ligation. The present study investigates the feasibility of a new application that can eliminate these limitations. Rapidly absorbable suture materials were used for ligation; therefore, spontaneous biliary decompression was anticipated by the self release of these rapidly degrading materials. Methods: Common bile ducts in Wistar Albino rats were ligated with silk, polyglytone 6211, or irradiated polyglactine 910 (n=7 for each group). Rats were grouped according to both the suture materials and the experiments termination date: 5 days (sham, silk5, polyglytone5, polyglactine5) and 21 days (silk21, polyglytone21, polyglactine21) after the ligation. Biochemical and morphologic changes of liver were assessed. Results: The group polyglactine21 showed significantly lower mean ALT, AST, GGT, total and direct bilirubin values when compared with the group polyglactine5 (p=0.004-0.037). Morphologic changes did not correlate with the biochemical amelioration. In the group polyglytone21, not only the biochemical but also the morphologic changes significantly ameliorated when compared with the group polyglytone5 (p=0.003-0.043). No procedure associated mortality was observed. Conclusion: Common bile duct ligation with polyglytone offers a new reversible model for prolonged obstructive jaundice which abolishes the need for relaparotomy and a second surgical intervention and significantly reduces mortality.

Peripheral vascular neuroeffector mechanisms in experimental cholestasis

1993 ◽  
Vol 265 (3) ◽  
pp. G579-G586 ◽  
Author(s):  
G. Jacob ◽  
O. Said ◽  
J. Finberg ◽  
A. Bomzon
Keyword(s):  
Electrical Stimulation ◽  
Bile Duct ◽  
Vascular Reactivity ◽  
Bile Duct Ligation ◽  
Norepinephrine Uptake ◽  
Pithed Rats ◽  
Duct Ligation ◽  
Adrenoceptor Agonists

Jaundiced patients have systemic hypotension and are more susceptible to hemorrhagic shock than nonjaundiced individuals. We have hypothesized that the mechanism whereby these cardiovascular complications arise is linked to a disturbance of the vascular neuroeffector process in the cardiovascular system. With the use of 3-day bile duct-manipulated (sham-operated) and bile duct-ligated rats, we have evaluated alpha-adrenoceptor function and amine uptake using in vivo and in vitro techniques. Blunted pressor responsiveness to norepinephrine, electrical stimulation, and the alpha 1-adrenoceptor agonists, methoxamine and phenylephrine, was observed in the bile duct-ligated pithed rats. In contrast, normal responsiveness to BHT-933 and clonidine, the alpha 2-adrenoceptor agonists, was seen in these animals. The uptake 1 blocker, cocaine, caused potentiation of equal magnitudes of the pressor responsiveness to electrical stimulation and norepinephrine in the sham-operated and bile duct-ligated pithed rats. In aortic rings prepared from the bile duct-ligated rats, blunted in vitro vascular reactivity to norepinephrine and the same alpha 1-adrenoceptor agonists was seen. Bile duct ligation had no effect on norepinephrine uptake or its kinetics in stressed and unstressed arterial rings and portal veins. We have thus concluded that bile duct ligation induces a defect in the functional expression of cardiovascular alpha 1-adrenoceptors without any effects on the activity of alpha 2-adrenoceptors or norepinephrine uptake.

Inhibition of the biosynthesis of uroporphyrinogen and heme in rat liver during obstructive jaundice produced by bile duct ligation

1986 ◽  
Vol 246 (1) ◽  
pp. 143-148 ◽  
Author(s):  
Walter N. Piper ◽  
James Tse ◽  
Emily M. Sadler ◽  
W.Russ Christenson ◽  
James L. Balk ◽  
...  
Keyword(s):  
Bile Duct ◽  
Obstructive Jaundice ◽  
Rat Liver ◽  
Bile Duct Ligation ◽  
Duct Ligation

scholarly journals Increased Cardiac Endocrine Activity After Common Bile Duct Ligation in the Rabbit Atrial Endocrine Cells in Obstructive Jaundice

1994 ◽  
Vol 219 (1) ◽  
pp. 73-78 ◽  
Author(s):  
J. A. Pereira ◽  
M. A. Torregrosa ◽  
F. Martínez-Ródenas ◽  
J. Clàrla ◽  
L. Pallarés ◽  
...  
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Obstructive Jaundice ◽  
Endocrine Cells ◽  
Bile Duct Ligation ◽  
Common Bile Duct Ligation ◽  
Endocrine Activity ◽  
Duct Ligation

scholarly journals MicroRNA-29a mitigation of endoplasmic reticulum and autophagy aberrance counteracts in obstructive jaundice-induced fibrosis in mice

2017 ◽  
Vol 243 (1) ◽  
pp. 13-21 ◽  
Author(s):  
Ying-Hsien Huang ◽  
Ya-Ling Yang ◽  
Fu-Chen Huang ◽  
Mao-Meng Tiao ◽  
Yen-Cheng Lin ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Bile Duct ◽  
Protein Expression ◽  
Bile Duct Ligation ◽  
Binding Protein ◽  
Wild Type ◽  
Innovative Strategy ◽  
Over Expression ◽  
Duct Ligation

Hepatic fibrosis was caused by a number of signaling pathways that damage liver integrity. We have previously shown that microRNA-29a (miR-29a) protects against liver fibrosis. Aberrant endoplasmic reticulum (ER) and autophagy function reportedly exaggerate hepatic disorders. The aim of this study was to characterize the biological influence of miR-29a on ER function in injured livers with bile duct ligation (BDL). We performed BDL on miR-29a transgenic mice (miR-29aTg) and wild-type mice to induce cholestatic liver injury. Rat T6 cells were transfected with miR-29a mimic and tunicamycin. Compared to the wild-type mice, the BDL deterioration of liver function in terms of total bilirubin, alanine transaminase, and aspartate transaminase activity in the miR-29aTg mice was significantly reduced. Affected livers in the miR-29aTg mice demonstrated a slight fibrotic matrix formation. miR-29a over-expression reduced the BDL disturbance of the expressions of inositol-requiring kinase 1alpha, double-stranded RNA-activated protein kinase-like endoplasmic reticulum kinase, spliced-X-box binding protein 1 (sXBP1), CCAAT/enhancer-binding protein homologous protein (CHOP), ULK, LC3BII, p62, and cleaved caspase-8, 9 and 3. In vitro, T6 cells exposed to tunicamycin by increasing abundances of CHOP, sXBP1, cleaved caspase-3, and LC3BII were diminished in the cell cultures transfected with the miR-29a mimic. On the other hand, we observed that miR-29a signaling protected liver tissues from BDL-mediated metabolic dysfunction and excessive fibrosis histopathology. This study provides new molecular insight into the miR-29a stabilization of ER integrity that slows the progression of cholestatic liver deterioration. Impact statement Long-term hepatic damage caused by hepatitis and cholestasis can accelerate fibrosis matrix over-production, which is a harmful process attributed to the dysregulation of a number of cellular and molecular events. The purpose of this study is to characterize the biological influence of miR-29a on endoplasmic reticulum (ER) function in bile duct ligation (BDL)-injured livers. To the best of our knowledge, this report is the first demonstration that miR-29a over-expression diminishes BDL provocation of ER stress (unfolded protein response, UPR) effector protein expression. This work also demonstrates that miR-29a decreased caspases protein expression in cholestatic livers, while an increase in miR-29a function reduced sXBP1 and CHOP expressions in T6 cells in mice. Analyses of this study highlight that controlling miR-29a signaling can serve as an innovative strategy in the future for microRNA regulation of ER homeostasis to combat cholestasis induction hepatic disorders.

Iepatic vitamin D 25-hydroxylase: Inhibition by bile duct ligation or bile salts

Hepatology ◽  
1981 ◽  
Vol 1 (5) ◽  
pp. 436-440 ◽  
Author(s):  
Erry J. G. Bolt ◽  
Michael D. Sitrin ◽  
Murray J. Favus ◽  
Irwin H. Rosenberg
Keyword(s):  
Vitamin D ◽  
Bile Duct ◽  
Bile Salts ◽  
Bile Duct Ligation ◽  
Duct Ligation

Haemodynamic Studies in Dogs with Chronic Bile-Duct Ligation

1976 ◽  
Vol 50 (6) ◽  
pp. 533-537 ◽  
Author(s):  
S. M. Shasha ◽  
O. S. Better ◽  
C. Chaimovitz ◽  
J. Doman ◽  
Y. Kishon
Keyword(s):  
Bile Duct ◽  
Bile Duct Ligation ◽  
Venous Pressure ◽  
Peripheral Resistance ◽  
Significant Rise ◽  
Cirrhosis Of The Liver ◽  
Sodium Retention ◽  
Oedema Formation ◽  
Duct Ligation

1. Dogs with bile-duct ligation retain salt and water and form ascites. The present study was undertaken to examine the role of haemodynamic factors in the aetiology of this sodium retention. 2. Haemodynamic studies were performed in five dogs before and 5 weeks after bile-duct ligation. 3. After the operation there was an insignificant fall in mean arterial pressure, a significant rise in mean cardiac index and a significant fall in mean total peripheral resistance. 4. It is concluded that heart failure is not a factor in renal sodium retention of the dog with bile-duct ligation, since the central venous pressure was not elevated. 5. The haemodynamic pattern and the tendency to salt retention in the dog with chronic bile-duct ligation closely resemble findings reported in patients with cirrhosis of the liver, and it is suggested that oedema formation in patients with cirrhosis of the liver and dogs with chronic bile-duct ligation shares a common aetiology.

Sign in / Sign up

Export Citation Format

Share Document