Serum Inorganic Fluoride: Changes Related to Previous Fluoride Intake, Renal Function and Bone Resorption

1980 ◽  
Vol 58 (2) ◽  
pp. 145-152 ◽  
Author(s):  
Christine Waterhouse ◽  
D. Taves ◽  
A. Munzer
Keyword(s):  
Renal Function ◽  
Bone Resorption ◽  
Creatinine Clearance ◽  
Glomerular Filtration ◽  
Fluoride Concentration ◽  
Human Organism ◽  
Inorganic Fluoride ◽  
Fluoride Intake ◽  
Urine Specimens ◽  
Serum And Urine

1. Inorganic fluoride concentrations were determined in serum and urine specimens of 24 subjects receiving a standardized low fluoride intake. Serum fluoride was directly correlated with previous intake and appeared to reflect bone fluoride stores. 2. A positive correlation between creatinine and fluoride clearance was found. However, striking reductions in fluoride clearance, which resulted in increases in serum fluoride, were not usually seen until the creatinine clearance was below 25 ml/min. 3. Parathormone produced an increase in serum fluoride and thyrocalcitonin a decrease, probably by their action on bone. 4. Six patients with chronic increased bone resorption had elevated fluoride concentrations. In five, when treatment was successful, serum fluoride fell. Interpretation of the data from this group of patients is complicated by initially low filtration rates associated with hypercalcaemia and hypercalciuria. 5. The sensitivity of the serum fluoride concentration to previous intake, glomerular filtration and the intensity of bone resorption suggests that the human organism exerts no direct homeostatic control over this ion.

Assessment of renal function by serum creatinine and creatinine clearance: glomerular filtration rate estimated by four procedures.

1989 ◽  
Vol 35 (12) ◽  
pp. 2326-2330 ◽  
Author(s):  
F Van Lente ◽  
P Suit
Keyword(s):  
Renal Function ◽  
Glomerular Filtration Rate ◽  
Serum Creatinine ◽  
Creatinine Clearance ◽  
Glomerular Filtration ◽  
Filtration Rate ◽  
Wide Range ◽  
Clear Method ◽  
Serum And Urine

Abstract We compared creatinine concentrations in serum and urine and creatinine clearances determined by two Jaffé (Beckman's "Astra," Boehringer Mannheim Diagnostics) and two enzymatic (Kodak, Boehringer Mannheim Diagnostics) methods. Serum creatinine and creatinine clearances determined by each method were also compared with the glomerular filtration rate as measured with use of sodium [125I]iothalamate in patients with a wide range of renal function. Results between methods correlated excellently, but we saw clear method-dependent biases of up to 2.9 mg/L for serum. The highest serum creatinine values and the lowest creatinine clearances were obtained with Boehringer Mannheim Diagnostics' Jaffé method. The reciprocal of the serum creatinine and the creatinine clearance also correlated well with the glomerular filtration rate, but all methods over-estimated the glomerular filtration rates to varying degrees. Appropriate standardization of methods appears to be as important as method principle for establishing an accurate relationship between creatinine determinations and glomerular filtration rate.

Assessment of renal function by inulin clearance: comparison with creatinine clearance as determined by enzymatic methods.

1989 ◽  
Vol 35 (2) ◽  
pp. 312-314 ◽  
Author(s):  
F S Apple ◽  
P Benson ◽  
P A Abraham ◽  
T G Rosano ◽  
C E Halstenson
Keyword(s):  
Renal Function ◽  
Glomerular Filtration Rate ◽  
Creatinine Clearance ◽  
Glomerular Filtration ◽  
Filtration Rate ◽  
The Other ◽  
Inulin Clearance ◽  
Urinary Creatinine ◽  
Single Slide ◽  
Enzymatic Methods

Abstract We compared creatinine clearances determined by enzymatic (Kodak Ektachem 700 single-slide, Boehringer Mannheim creatinine PAP) and nonenzymatic (Jaffé, HPLC) methods with glomerular filtration rate measured by inulin clearance in patients with varying degrees of renal function. The Kodak enzymatic assay gave values for creatinine 2 to 3 mg/L higher than the other methods. This resulted in significantly lower creatinine clearances than inulin clearances and creatinine clearances determined by the other methods. However, correlations between all methods for serum and urinary creatinine values and clearances were good. To avoid between assay (enzymatic vs nonenzymatic) discrepancies, manufacturers should agree to an acceptable standard of calibration under the usual conditions used with patients.

Renal Clearance of Unbound Bilirubin in the Rat

1977 ◽  
Vol 53 (2) ◽  
pp. 193-196 ◽  
Author(s):  
María Mónica Elías ◽  
E. J. Comin ◽  
E. A. Rodriguez Garay
Keyword(s):  
Creatinine Clearance ◽  
Glomerular Filtration ◽  
Gel Filtration ◽  
Bilirubin Concentration ◽  
Filtration Method ◽  
Duct Ligation ◽  
Unbound Bilirubin ◽  
The Difference ◽  
Filtered Load ◽  
Serum And Urine

1. Unbound or diffusible bilirubin in serum and urine was measured in rats with bile-duct ligation or after continuous infusion of unconjugated bilirubin. 2. The glomerular filtration rate was estimated by measuring the endogenous creatinine clearance and the unbound bilirubin in serum and urine was determined by a Sephadex gel filtration method. 3. Unbound bilirubin clearance was significantly lower than creatinine clearance. 4. The estimated rate of bilirubin reabsorption (calculated from the difference between the unbound bilirubin filtered load and the bilirubin excreted in the urine) was directly related to the serum unbound bilirubin concentration and the unbound bilirubin filtered load. 5. The results suggest that the main mechanism of urinary excretion of bilirubin in the rat involves glomerular filtration and tubular reabsorption by diffusion.

Pseudouridine is unsuitable as an endogenous renal clearance marker.

1988 ◽  
Vol 34 (6) ◽  
pp. 1011-1017 ◽  
Author(s):  
J T Bernert ◽  
C J Bell ◽  
J Guntupalli ◽  
W H Hannon
Keyword(s):  
High Performance Liquid Chromatography ◽  
Glomerular Filtration Rate ◽  
Glomerular Filtration ◽  
High Performance ◽  
Filtration Rate ◽  
Healthy Men ◽  
Experimental Animals ◽  
Potential Use ◽  
Urine Specimens ◽  
Serum And Urine

Abstract We assessed the clearance of endogenous pseudouridine in humans to evaluate the potential use of this modified nucleoside as a marker of glomerular filtration rate. Pseudouridine concentrations in serum ultrafiltrates and in the corresponding 24-h urine specimens from 19 healthy men were determined by high-performance liquid chromatography. Mean (and SD) pseudouridine concentrations in serum and urine from this group averaged 2.77 (0.34) mumol/L and 203.2 (64.8) mumol/L, respectively. The calculated clearances of the nucleoside [87.3 (24.9) mL/min, n = 19], however, averaged approximately one-third lower than the corresponding creatinine clearances in the same individuals [131.8 (28.4) mL/min]. Measurement of simultaneous clearances of [3H]pseudouridine and [14C]inulin in rats also yielded a lower pseudouridine clearance, 0.78 relative to inulin. Our results are thus consistent with a partial net reabsorption of pseudouridine in both experimental animals and in humans, indicating that this compound would not be a suitable endogenous marker for routine estimation of the glomerular filtration rate.

Apolipoprotein(a) is Present in Urine and Its Excretion is Decreased in Patients with Renal Failure

1992 ◽  
Vol 38 (11) ◽  
pp. 2244-2248 ◽  
Author(s):  
K Oida ◽  
H Takai ◽  
H Maeda ◽  
S Takahashi ◽  
A Shimada ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Renal Failure ◽  
Renal Function ◽  
Serum Creatinine ◽  
Creatinine Clearance ◽  
Renal Dysfunction ◽  
Filtration Rate ◽  
Apolipoprotein A ◽  
Patients With Diabetes ◽  
Serum And Urine

Abstract To investigate the relation between renal function and concentrations of lipoprotein(a) [Lp(a)] in serum, we measured Lp(a) in samples of serum and urine from patients with diabetes mellitus and in samples sent to a laboratory center for measurements of creatinine clearance. Serum Lp(a) concentrations were significantly increased in subjects with obvious renal dysfunction (serum creatinine > or = 176.8 mumol/L) compared with normal control subjects. Urinary Lp(a) excretion was decreased in subjects with obvious renal dysfunction compared with subjects without obvious renal dysfunction (serum creatinine < or = 88.4 mumol/L) and was negatively and positively correlated with serum creatinine and creatinine clearance, respectively. More than 80% of urinary Lp(a) was recovered in the d > 1.21 kg/L fraction. At least six bands for apolipoprotein(a) [apo(a)] fragments, which were smaller than native apo(a) in serum, were observed in urine by immunoblotting, and some of these were also detected in serum. Degraded apo(a) fragments are probably present in urine, and their excretion decreases in parallel with decreases in the glomerular filtration rate.

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