Rapid Transfer of Folic Acid from Blood to Bile in Man, and its Conversion into Folate Coenzymes and into a Pteroylglutamate with Little Biological Activity

1974 ◽  
Vol 46 (6) ◽  
pp. 729-741 ◽  
Author(s):  
A. Lavoie ◽  
B. A. Cooper
Keyword(s):  
Biological Activity ◽  
Folic Acid ◽  
Rose Bengal ◽  
Lactobacillus Casei ◽  
Streptococcus Faecalis ◽  
Human Bile ◽  
Methylene Tetrahydrofolate ◽  
Special Modification ◽  
A Chain ◽  
Rapid Transfer

1. [3H]Folic acid infused intravenously into patients with biliary fistulae appeared promptly in bile, coincident with 131I-labelled Rose Bengal injected simultaneously. The radioactivity was distributed among several fractions of biological folates present in bile and was associated with folic acid and with an unidentified folate which chromatographed on DEAE-Sephadex close to 10-formyltetrahydrofolyl monoglutamate. 2. Based on affinity for DEAE-Sephadex and support of growth of Lactobacillus casei, Streptococcus faecalis and Pediococcus cerevisiae, we have tentatively identified some of the folates of human bile as 10-formyltetrahydrofolate, 10-formylfolate, 5-formyltetrahydrofolate, 5-methyltetrahydrofolate and tetrahydrofolate or 5,10-methylene tetrahydrofolate. After infusion of folic acid, the formyltetrahydrofolates increased more rapidly than did 5-methyltetrahydrofolate. 3. The unidentified radioactive folate contained both the pteridine and p-aminobenzoate portions of folate. It appeared not to support growth of the test microorganisms and not to be bound to a protein or a chain of γ-glutamates. It was present in the bile of a dog injected with [3H]folic acid but was absent from extracts of liver. This material may be a transport form of folate or a special modification imposed on folic acid during transport across the liver.

5-Methyltetrahydrofolic acid and other folate derivatives in germinating pea seedlings

1968 ◽  
Vol 46 (12) ◽  
pp. 1533-1536 ◽  
Author(s):  
A. J. Roos ◽  
A. M. Spronk ◽  
E. A. Cossins
Keyword(s):  
Folic Acid ◽  
Lactobacillus Casei ◽  
Deae Cellulose ◽  
Methyl Derivative ◽  
Streptococcus Faecalis ◽  
Feeding Experiments ◽  
Pea Seedlings ◽  
Young Leaves

The folate derivatives present in germinating pea seedlings were isolated by chromatography on DEAE-cellulose and assayed with Lactobacillus casei, Streptococcus faecalis, and Pediococcus cerevisiae. The major folate derivative in the cotyledons, developing embryos, and young leaves was identified as 5-methyltetrahydrofolate. Smaller amounts of 10-formyltetrahydrofolate were also present in these tissues. Synthesis of these derivatives in the cotyledons was inhibited by aminopterin. Feeding experiments showed that the 5-methyl derivative was rapidly synthesized from folic acid-2-14C.

The biological activity of fermented milk produced by Lactobacillus casei ATCC 393 during cold storage

2019 ◽  
Vol 91 ◽  
pp. 1-8 ◽  
Author(s):  
Mahmoud Abdel-Hamid ◽  
Ehab Romeih ◽  
Raúl Ricardo Gamba ◽  
Emiko Nagai ◽  
Takuya Suzuki ◽  
...  
Keyword(s):  
Biological Activity ◽  
Cold Storage ◽  
Lactobacillus Casei ◽  
Fermented Milk

Improved Microbiological Assay for Folic Acid Based on Microtiter Plating with Streptococcus faecalis

1995 ◽  
Vol 78 (5) ◽  
pp. 1173-1177 ◽  
Author(s):  
Jayasri Das Sarma ◽  
Chandralekha Duttagupta ◽  
Esahak Ali ◽  
Tarun K Dhar
Keyword(s):  
Folic Acid ◽  
Direct Measurement ◽  
Microbiological Assay ◽  
Bacterial Suspension ◽  
Microbiological Analysis ◽  
Improved Method ◽  
Streptococcus Faecalis ◽  
Microtiter Plates ◽  
Assay Time

Abstract We have developed an improved method, using 96-well microtiter plates, for the microbiological assay of folic acid. With this method, the tedium of conventional microbiological analysis is substantially decreased. Culture volumes have been reduced 33-fold, and pipetting procedures have been simplified. Assay time has been reduced to 14 h, and sensitivity has increased 10-fold (0.1 ng/mL). Analytical recoveries range from 98 to 104%. Intra-assay and interassay variabilities are less than 11%. The assay does not require extensive manipulation of inoculum. Day-to-day variability has been minimized by using saline aliquots of the bacterial suspension stored at 4°C. The procedure is accurate, selective, and useful for direct measurement of folic acid in multivitamin formulations.

A Microbiologic Method for Detecting Folic Acid Deficiency in Man

1959 ◽  
Vol 5 (4) ◽  
pp. 275-280 ◽  
Author(s):  
Herman Baker ◽  
V Herbert ◽  
O Frank ◽  
I Pasher ◽  
S H Hutner ◽  
...  
Keyword(s):  
Folic Acid ◽  
Glutamic Acid ◽  
Clinical Diagnosis ◽  
Lactobacillus Casei ◽  
Folic Acid Deficiency ◽  
Acid Deficiency ◽  
Acid Status ◽  
Assay Methods

Abstract A microbiologic method based on Lactobacillus casei for assaying folic acid activity in serum is presented. The assay correlates directly with the clinical diagnosis. Comparison with formimino-L-glutamic acid determinations and other microbiologic assay methods shows the assay here described to yield a more dependable estimate of the folic acid status in humans.

Biological activity of recombinantRicinus communisagglutinin A chain produced inEscherichia coli

FEBS Letters ◽  
1992 ◽  
Vol 299 (3) ◽  
pp. 209-212 ◽  
Author(s):  
Mary O'Hare ◽  
Lynne M. Roberts ◽  
J.Michael Lord
Keyword(s):  
Biological Activity ◽  
Inescherichia Coli ◽  
A Chain

Biological Activity of Natural and Synthetic Insulin A-chain Preparations on the Isolated Rat Diaphragm

Nature ◽  
1964 ◽  
Vol 203 (4943) ◽  
pp. 408-409 ◽  
Author(s):  
P. VOLFIN ◽  
M. CHAMBAUT ◽  
D. EBOUÉ-BONIS ◽  
H. CLAUSER ◽  
O. BRINKHOFF ◽  
...  
Keyword(s):  
Biological Activity ◽  
Rat Diaphragm ◽  
A Chain ◽  
Isolated Rat ◽  
Natural And Synthetic

scholarly journals Folic acid metabolism in vitamin B12-deficient sheep. Effects of injected methionine on liver constituents associated with folate metabolism

1974 ◽  
Vol 142 (1) ◽  
pp. 105-117 ◽  
Author(s):  
Richard M. Smith ◽  
William S. Osborne-White ◽  
Jeffrey M. Gawthorne
Keyword(s):  
Folic Acid ◽  
Ion Exchange ◽  
Vitamin B12 ◽  
Lactobacillus Casei ◽  
Acid Metabolism ◽  
Major Effect ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Folate Metabolism ◽  
Folic Acid Metabolism

1. The effects of injected l-methionine (2g every second day for 28 days) on liver folates and other constituents of liver associated with folate metabolism were studied in vitamin B12-deficient ewes and their pair-fed controls receiving vitamin B12. The dose rate of methionine used was sufficient to restore almost to normal the elevated excretion in the urine of formiminoglutamate in the deficient animals. 2. Liver folates active for Lactobacillus casei, Streptococcus faecalis R and Pediococcus cerevisiae were severely depressed in deficient livers and were partly restored by methionine. Analysis of the folates after ion-exchange chromatography showed that the major effect of methionine was to increase the concentrations of tetrahydrofolates and formyltetrahydrofolates. Methyltetrahydrofolates were also increased, but there was no effect of methionine on the small amounts of incompletely reduced folates present in deficient livers. The folates present were predominantly penta-, hexa- and hepta-glutamates whether or not animals received vitamin B12 or methionine. 3. Concentrations of ATP, NAD+, NADH and NADPH were lower in freeze-clamped liver from vitamin B12-deficient sheep than in liver from pair-fed, vitamin B12-treated sheep. These changes were not affected by methionine which was also without effect on the elevated K+/Na+ ratios found in deficient livers. 4. The livers of vitamin B12-deficient animals contained lower concentrations of choline and higher concentrations of lipid than their pair-fed controls. These effects were reversed by methionine.

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