Biological Activity of Natural and Synthetic Insulin A-chain Preparations on the Isolated Rat Diaphragm

Nature ◽  
1964 ◽  
Vol 203 (4943) ◽  
pp. 408-409 ◽  
Author(s):  
P. VOLFIN ◽  
M. CHAMBAUT ◽  
D. EBOUÉ-BONIS ◽  
H. CLAUSER ◽  
O. BRINKHOFF ◽  
...  
Keyword(s):  
Biological Activity ◽  
Rat Diaphragm ◽  
A Chain ◽  
Isolated Rat ◽  
Natural And Synthetic

SENSITIVITY OF THE RAT DIAPHRAGM TO GROWTH HORMONE.

1967 ◽  
Vol 54 (4) ◽  
pp. 645-662 ◽  
Author(s):  
Å. Hjalmarson ◽  
K. Ahrén
Keyword(s):  
Growth Hormone ◽  
Inhibitory Effect ◽  
Rat Diaphragm ◽  
Intracellular Accumulation ◽  
Slight Effect ◽  
Kidney Capsule ◽  
Muscle Tissues ◽  
Hypophysectomized Rats ◽  
Isolated Rat

ABSTRACT The effect of growth hormone (GH) in vitro on the rate of intracellular accumulation of the non-utilizable amino acid α-aminoisobutyric acid (AIB) was studied in the intact rat diaphragm preparation. Bovine or ovine GH (25 μg/ml incubation medium) markedly stimulated the accumulation of AIB-14C by diaphragms from hypophysectomized rats, while there was no or only a very slight effect on diaphragms from normal rats. In diaphragms from rats with the pituitary gland autotransplanted to the kidney capsule GH in vitro stimulated the accumulation of AIB-14C significantly more than in diaphragms from normal rats but significantly less than in diaphragms from hypophysectomized rats. Injections of GH intramuscularly for 4 days to hypophysectomized rats made the diaphragms from these rats less sensitive or completely insensitive to GH in vitro. These results indicate strongly that the relative insensitivity to GH in vitro of diaphragms from normal rats is due to the fact that the muscle tissues from these rats has been exposed to the endogenously secreted GH. The results show that GH can influence the accumulation of AIB-14C in the isolated rat diaphragm in two different ways giving an acute or »stimulatory« effect and a late or »inhibitory« effect, and that it seems to be a time-relationship between these two effects of the hormone.

scholarly journals PHOSPHATE METABOLISM OF THE ISOLATED RAT DIAPHRAGM

1951 ◽  
Vol 189 (1) ◽  
pp. 59-63
Author(s):  
Niels. Haugaard ◽  
Julian B. Marsh ◽  
William C. Stadie
Keyword(s):  
Phosphate Metabolism ◽  
Rat Diaphragm ◽  
Isolated Rat

scholarly journals Rb1.66Cs1.34Tb[Si5.43Ge0.57O15]·H2O, a New Member of the OD-Family of Natural and Synthetic Layered Silicates: Topology-Symmetry Analysis and Structure Prediction

Minerals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 395
Author(s):  
Anastasiia Topnikova ◽  
Elena Belokoneva ◽  
Olga Dimitrova ◽  
Anatoly Volkov ◽  
Dina Deyneko
Keyword(s):  
Structure Prediction ◽  
Symmetry Analysis ◽  
Layered Silicates ◽  
Water Molecules ◽  
Structural Variants ◽  
X Ray ◽  
A Chain ◽  
Multi Component System ◽  
Tetrahedral Layer ◽  
Natural And Synthetic

Crystals of new silicate-germanate Rb1.66Cs1.34Tb[Si5.43Ge0.57O15]·H2O have been synthesized hydrothermally in a multi-component system TbCl3:GeO2:SiO2 = 1:1:5 at T = 280 °C and P = 100 atm. K2CO3, Rb2CO3 and Cs2CO3 were added to the solution as mineralizers. The crystal structure was solved using single crystal X-ray data: a = 15.9429(3), b = 14.8407(3), c = 7.2781(1) Å, sp. gr. Pbam. New Rb,Cs,Tb-silicate-germanate consists of a [Si5.43Ge0.57O15]∞∞ corrugated tetrahedral layer combined by isolated TbO6 octahedra into the mixed microporous framework as in synthetic K3Nd[Si6O15]·2H2O, K3Nd[Si6O15] and K3Eu[Si6O15]·2H2O with the cavities occupied by Cs, Rb atoms and water molecules. Luminescence spectrum on new crystals was obtained and analysed. A comparison with the other representatives of related layered natural and synthetic silicates was carried out based on the topology-symmetry analysis by the OD (order-disorder) approach. The wollastonite chain was selected as the initial structural unit. Three symmetrical ways of forming ribbon from such a chain and three ways of further connecting ribbons to each other into the layer were revealed and described with symmetry groupoids. Hypothetical structural variants of the layers and ribbons in this family were predicted.

scholarly journals THE EFFECT OF GROWTH HORMONE ON GLUCOSE UPTAKE BY THE ISOLATED RAT DIAPHRAGM

1952 ◽  
Vol 197 (1) ◽  
pp. 151-166 ◽  
Author(s):  
C.R. Park ◽  
David H. Brown ◽  
Marvin. Cornblath ◽  
William H. Daughaday ◽  
M.E. Krahl
Keyword(s):  
Growth Hormone ◽  
Glucose Uptake ◽  
Rat Diaphragm ◽  
Isolated Rat

ANTAGONISM BETWEEN INSULIN AND SELECTIVE ADRENERGIC AGONISTS ON THE GLYCOGEN SYNTHETASE AND PHOSPHORYLASE SYSTEMS OF THE ISOLATED RAT DIAPHRAGM

1975 ◽  
Vol 78 (2) ◽  
pp. 392-400
Author(s):  
Arne T. Hostmark ◽  
Ole Grønnerød ◽  
Robert S. Horn
Keyword(s):  
Glycogen Metabolism ◽  
Rat Diaphragm ◽  
Adrenergic Agonists ◽  
Adrenergic Stimulation ◽  
Insulin Effect ◽  
Glycogen Synthetase ◽  
Adrenergic Antagonists ◽  
Fasted Rats ◽  
Isolated Rat ◽  
Stimulation Of

ABSTRACT The antagonism between insulin and selective adrenergic stimulation on the converting systems for glycogen synthetase and phosphorylase has been investigated in the isolated rat diaphragm. Insulin significantly inhibited stimulation by terbutaline and noradrenaline of phosphorylase b to a conversion as well as stimulation of glycogen synthetase I to D conversion by these agents. The inhibition by insulin was stronger on the synthetase system than on the phosphorylase system. The insulin effect was not dependent upon the presence of glucose. In diaphragms from 24 h fasted rats the response of the phosphorylase system to both agonists decreased. Inhibition by insulin of terbutaline stimulated phosphorylase conversion was maintained upon fasting while no effect of insulin against stimulation by noradrenaline could be obtained in diaphragms from fasted rats. The effects of fasting and insulin were not influenced by beta adrenergic antagonists (practolol and butoxamine). The results indicate a difference in sensitivity of the synthetase and phosphorylase systems to insulin and suggest that noradrenaline and terbutaline influence glycogen metabolism by differing mechanisms.

scholarly journals Metabolism of hexose phosphate esters. 1. Metabolism in normal and alloxan-diabetic rabbits. 2. Metabolism in isolated rat diaphragm and the influence of insulin

1953 ◽  
Vol 54 (4) ◽  
pp. 529-539 ◽  
Author(s):  
Anne Beloff-Chain ◽  
E. B. Chain ◽  
D. Bovet ◽  
F. Pocchiari ◽  
R. Catanzaro ◽  
...  
Keyword(s):  
Phosphate Esters ◽  
Rat Diaphragm ◽  
Hexose Phosphate ◽  
Diabetic Rabbits ◽  
Isolated Rat
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