Fatty Acid Synthesis De Novo in Human Adipose Tissue

1974 ◽  
Vol 46 (4) ◽  
pp. 469-479
Author(s):  
R. B. Goldrick ◽  
D. J. Galton
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Subcutaneous Adipose Tissue ◽  
De Novo ◽  
Fatty Acid Synthetase ◽  
Human Adipose Tissue ◽  
Acid Synthesis ◽  
Cleavage Enzyme ◽  
Subcutaneous Adipose

1. Homogenates of subcutaneous adipose tissue from obese subjects contained sufficient citrate cleavage enzyme (EC 4.1.3.8: ATP—citrate oxaloacetate lyase), acetyl-CoA carboxylase (EC 6.4.1.2: acetyl-CoA—carbon dioxide ligase) and fatty acid synthetase to account for the rates of fatty acid synthesis reported in the literature for intact tissues in vitro. 2. The distribution of label in fatty acids after incubations of adipose tissue with [1-14C]acetate was consistent with fatty acid synthesis de novo, not chain elongation. 3. The activities of citrate cleavage enzyme and fatty acid synthetase in subcutaneous adipose tissue were reduced by short periods of starvation. 4. These findings indicate that human adipose tissue is capable of fatty acid synthesis de novo and that two of the enzymes concerned adapt to starvation.

scholarly journals Downregulation of de Novo Fatty Acid Synthesis in Subcutaneous Adipose Tissue of Moderately Obese Women

2015 ◽  
Vol 16 (12) ◽  
pp. 29911-29922 ◽  
Author(s):  
Esther Guiu-Jurado ◽  
Teresa Auguet ◽  
Alba Berlanga ◽  
Gemma Aragonès ◽  
Carmen Aguilar ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Subcutaneous Adipose Tissue ◽  
De Novo ◽  
Acid Synthesis ◽  
Obese Women ◽  
Subcutaneous Adipose

Genetic variations in subcutaneous adipose tissue metabolism in sheep

1988 ◽  
Vol 47 (2) ◽  
pp. 263-270 ◽  
Author(s):  
P. A. Sinnett-Smith ◽  
J. A. Woolliams
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Lipoprotein Lipase ◽  
Fatty Acid Synthesis ◽  
Subcutaneous Adipose Tissue ◽  
Acid Synthesis ◽  
Acetyl Coa Carboxylase ◽  
Adipocyte Volume ◽  
Glycerol Synthesis ◽  
Subcutaneous Adipose

ABSTRACTAdipocyte volume rates of fatty acid synthesis, acylglycerol glycerol synthesis and lipolysis (basal and noradrenaline stimulated) along with the activities of acetyl CoA carboxylase and lipoprotein lipase were determined in subcutaneous adipose tissue, sampled by biopsy, from the rump of four breeds of sheep differing in growth and body characteristics.Significant differences among breeds were observed for adipocyte volume, fatty acid synthesis, stimulated lipolysis rates, initial and total acetyl CoA carboxylase activity and lipoprotein lipase activity, but not for acylglycerol glycerol synthesis.Differences in adipocyte volume did not appear to be related to the previously reported carcass fatnesses of the breeds. Similarly differences in adipocyte volume were not related to differences in either de novo fatty acid synthesis or lipolysis rates. Across breeds there was a trend toward higher acylglycerol glycerol synthesis rates associated with greater adipocyte volume although the source of fatty acids for esterification varied greatly.Breed variation in fatness in sheep therefore appears to be a consequence of different balances of anabolic and catabolic processes in adipose tissue, with a unique pattern for each breed. Further elucidation of these patterns may lead to the identification of key sites for genetic manipulation. In addition these breed differences provide an alternative, complementary and qualitatively different, model for the study of the control of fat metabolism to that provided by nutritional or hormonal manipulations.

scholarly journals The role of dietary protein in hepatic lipogenesis in the young rat

1981 ◽  
Vol 45 (3) ◽  
pp. 529-538 ◽  
Author(s):  
G. R. Herzberg ◽  
Minda Rogerson
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Dietary Protein ◽  
Fatty Acid Synthetase ◽  
Acid Synthesis ◽  
Hepatic Lipogenesis ◽  
Hepatic Fatty Acid ◽  
Cleavage Enzyme ◽  
Glucose 6 Phosphate Dehydrogenase

1. The effect of varying dietary levels of casein (40–140 g/kg) on hepatic lipogenesis and the levels of hepatic fatty acid synthetase (FAS), glucose-6-phosphate dehydrogenase (EC 1.1.1.49; G6PD), malic enzyme (EC 1.1.1.40; ME), citrate cleavage enzyme (EC 4.1.3.8;CCE), acetyl CoA carboxylase (EC 6.4.1.2; AcCx), glucokinase (EC 2.7.1.2; GK), and pyruvate dehydrogenase (PDH) was examined in young, growing rats.2. The activities of AcCx, FAS, G6PD and in vivo fatty acid synthesis were generally found to increase with increased dietary protein.3. The levels of GK and PDH were not related to dietary protein.4. ME decreased with increasing dietary protein.5. The results demonstrate a dissociation between hepatic fatty acid synthesis and ME and suggest that when rats consume low-protein diets the NADPH needed for fatty acid synthesis is generated primarily by ME but that as the level of dietary protein is increased the contribution of ME is reduced while that of the phosphogluconate pathway becomes more important.

Fatty acid synthesis by human adipose tissue

Metabolism ◽  
1975 ◽  
Vol 24 (2) ◽  
pp. 161-173 ◽  
Author(s):  
Mulchand S. Patel ◽  
Oliver E. Owen ◽  
Leonard I. Goldman ◽  
Richard W. Hanson
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Human Adipose Tissue ◽  
Acid Synthesis

scholarly journals Chronic ethanol administration depresses fatty acid synthesis in rat adipose tissue

1988 ◽  
Vol 251 (2) ◽  
pp. 547-551 ◽  
Author(s):  
J S Wilson ◽  
M A Korsten ◽  
L P Donnelly ◽  
P W Colley ◽  
J B Somer ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Fatty Acid Synthetase ◽  
Acid Synthesis ◽  
Chronic Ethanol ◽  
Ethanol Administration ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Chronic Ethanol Administration

Administration of ethanol as part of a nutritionally adequate liquid diet to female Wistar rats was found to depress markedly incorporation of labelled glucose into adipose-tissue acylglycerol fatty acids. Similar results with labelled pyruvate and acetate suggested inhibition of the fatty-acid-synthesis pathway at, or distal to, the acetyl-CoA carboxylase step. Activities of acetyl-CoA carboxylase and fatty acid synthetase were markedly lower in ethanol-fed animals. The activity of another lipogenic enzyme, phosphatidate phosphohydrolase, was not affected by chronic ethanol feeding. These findings suggest that chronic ethanol administration has marked effects on adipose-tissue lipogenesis.

Effects of pregnancy and ovarian steroids on fatty acid synthesis and uptake in Syrian hamsters

1991 ◽  
Vol 260 (1) ◽  
pp. R153-R158 ◽  
Author(s):  
A. J. Bhatia ◽  
G. N. Wade
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid ◽  
White Adipose Tissue ◽  
Fatty Acid Synthesis ◽  
De Novo ◽  
Acid Synthesis ◽  
De Novo Lipogenesis ◽  
Ovarian Steroids ◽  
Syrian Hamsters

The effects of pregnancy and ovarian steroids on the in vivo distribution of newly synthesized fatty acids (incorporation of tritium from 3H2O into fatty acid) in Syrian hamsters (Mesocricetus auratus) were examined. During late, but not early, gestation hamsters had reduced levels of newly synthesized fatty acids in heart, liver, uterus, and white adipose tissues (parametrial and inguinal fat pads). Treatment of ovariectomized hamsters with estradiol + progesterone significantly decreased fatty acid synthesis-uptake in heart, liver, and inguinal white adipose tissue. Treatment with either estradiol or progesterone alone was without significant effect in any tissue. Pretreatment of hamsters with Triton WR-1339 (tyloxapol), an inhibitor of lipoprotein lipase activity and tissue triglyceride uptake, abolished the effects of estradiol + progesterone in white adipose tissue and heart but not in liver. Thus hamsters lose body fat during pregnancy in part because of decreased de novo lipogenesis. The effect of pregnancy on lipogenesis is mimicked by treatment with estradiol + progesterone but not by either hormone alone. Furthermore, it appears that the liver is the principal site of estradiol + progesterone action on lipogenesis in Syrian hamsters.

scholarly journals Localization of chloroplastic fatty acid synthesis de novo in the stroma

1985 ◽  
Vol 226 (2) ◽  
pp. 551-556 ◽  
Author(s):  
K A Walker ◽  
J L Harwood
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
High Speed ◽  
De Novo ◽  
Fatty Acid Synthetase ◽  
Acid Synthesis ◽  
Synthetic Activity ◽  
Bisphosphate Carboxylase ◽  
Osmotic Lysis ◽  
Almost All

The synthesis of fatty acids de novo from [2-14C]malonyl-CoA was studied in fractions from lettuce (Lactuca sativa) and pea (Pisum sativum) chloroplasts. When lettuce chloroplasts were subjected to osmotic lysis, disintegration through a Yeda press and high-speed centrifugation, essentially all of the fatty-acid-synthetic activity was found to be soluble. The distribution of the activity in various chloroplast fractions was similar to that of soluble marker enzymes such as ribulose-1,5-bisphosphate carboxylase and NADP+-linked glyceraldehyde-3-phosphate dehydrogenase. Marked differences were apparent in the quality of products from fatty acid synthesis de novo in the various fractions of chloroplasts. Thus soluble fractions produced predominantly stearate, whereas those containing membranes produced a greater proportion of palmitate. In pea chloroplasts, osmotic lysis released almost all of the fatty acid synthetase into the stromal fraction. In this instance, no major alterations in the products of fatty acid synthesis were observed. The fatty-acid-synthetic activity of the stromal fraction was still soluble after prolonged ultracentrifugation. The results show clearly the soluble nature of fatty acid synthesis de novo in lettuce and pea chloroplasts. Thus fatty acid synthesis measured in microsomal fractions from such plant tissues is not due to the presence of chloroplastic membranes.

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