scholarly journals Clathrin and synaptic vesicle endocytosis: studies at the squid giant synapse

2006 ◽  
Vol 34 (1) ◽  
pp. 68-72 ◽  
Author(s):  
G.J. Augustine ◽  
J.R. Morgan ◽  
C.A. Villalba-Galea ◽  
S. Jin ◽  
K. Prasad ◽  
...  
Keyword(s):  
Synaptic Vesicle ◽  
Adaptor Proteins ◽  
Vesicle Recycling ◽  
Heat Shock Cognate 70 ◽  
Primary Means ◽  
Squid Giant Synapse ◽  
Membrane Budding ◽  
Giant Synapse ◽  
Dependent Pathway

The role of clathrin-mediated endocytosis in SV (synaptic vesicle) recycling has been studied by combining molecular biology, physiology and electron microscopy at the squid giant synapse. Procedures that prevent clathrin from assembling into membrane coats, such as impairment of binding of the AP180 and AP-2 adaptor proteins, completely prevent membrane budding during endocytosis. These procedures also reduce exocytosis, presumably an indirect effect of a reduction in the number of SVs following block of endocytosis. Disrupting the binding of auxilin to Hsc70 (heat-shock cognate 70) prevents clathrin-coated vesicles from uncoating and also disrupts SV recycling. Taken together, these results indicate that a clathrin-dependent pathway is the primary means of SV recycling at this synapse under physiological conditions.

scholarly journals Role of Rab27 in synaptic transmission at the squid giant synapse

2008 ◽  
Vol 105 (41) ◽  
pp. 16003-16008 ◽  
Author(s):  
E. Yu ◽  
E. Kanno ◽  
S. Choi ◽  
M. Sugimori ◽  
J. E. Moreira ◽  
...  
Keyword(s):  
Synaptic Transmission ◽  
Squid Giant Synapse ◽  
Giant Synapse

scholarly journals Essential Role of Phosphoinositide Metabolism in Synaptic Vesicle Recycling

Cell ◽  
1999 ◽  
Vol 99 (2) ◽  
pp. 179-188 ◽  
Author(s):  
Ottavio Cremona ◽  
Gilbert Di Paolo ◽  
Markus R Wenk ◽  
Anita Lüthi ◽  
Warren T Kim ◽  
...  
Keyword(s):  
Synaptic Vesicle ◽  
Essential Role ◽  
Phosphoinositide Metabolism ◽  
Synaptic Vesicle Recycling ◽  
Vesicle Recycling

Key role of clathrin-mediated endocytosis in synaptic vesicle recycling

2007 ◽  
Vol 39 (4-5) ◽  
pp. 339-340
Author(s):  
Y. Tsytsyura ◽  
N. Glyvuk ◽  
M. Krikunova ◽  
N. Jung ◽  
V. Haucke ◽  
...  
Keyword(s):  
Synaptic Vesicle ◽  
Synaptic Vesicle Recycling ◽  
Vesicle Recycling

scholarly journals The role of non-canonical SNAREs in synaptic vesicle recycling

2012 ◽  
Vol 2 (1) ◽  
pp. 20-27 ◽  
Author(s):  
Denise M.O. Ramirez ◽  
Ege T. Kavalali
Keyword(s):  
Synaptic Vesicle ◽  
Synaptic Vesicle Recycling ◽  
Vesicle Recycling

scholarly journals Colocalization of synapsin and actin during synaptic vesicle recycling

2003 ◽  
Vol 161 (4) ◽  
pp. 737-747 ◽  
Author(s):  
Ona Bloom ◽  
Emma Evergren ◽  
Nikolay Tomilin ◽  
Ole Kjaerulff ◽  
Peter Löw ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Synaptic Vesicle ◽  
Active Zone ◽  
Synaptic Vesicles ◽  
Synaptic Activity ◽  
Immunogold Electron Microscopy ◽  
Synaptic Vesicle Recycling ◽  
Vesicle Recycling ◽  
Reserve Pool ◽  
Giant Synapse

It has been hypothesized that in the mature nerve terminal, interactions between synapsin and actin regulate the clustering of synaptic vesicles and the availability of vesicles for release during synaptic activity. Here, we have used immunogold electron microscopy to examine the subcellular localization of actin and synapsin in the giant synapse in lamprey at different states of synaptic activity. In agreement with earlier observations, in synapses at rest, synapsin immunoreactivity was preferentially localized to a portion of the vesicle cluster distal to the active zone. During synaptic activity, however, synapsin was detected in the pool of vesicles proximal to the active zone. In addition, actin and synapsin were found colocalized in a dynamic filamentous cytomatrix at the sites of synaptic vesicle recycling, endocytic zones. Synapsin immunolabeling was not associated with clathrin-coated intermediates but was found on vesicles that appeared to be recycling back to the cluster. Disruption of synapsin function by microinjection of antisynapsin antibodies resulted in a prominent reduction of the cytomatrix at endocytic zones of active synapses. Our data suggest that in addition to its known function in clustering of vesicles in the reserve pool, synapsin migrates from the synaptic vesicle cluster and participates in the organization of the actin-rich cytomatrix in the endocytic zone during synaptic activity.

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