Transcription factors acting on the promoter of the fatty acid synthase gene

2002 ◽  
Vol 30 (5) ◽  
pp. A102-A102
Author(s):  
M. Schweizer ◽  
K. Roder ◽  
L. Zhang ◽  
S.S. Wolf
Keyword(s):  
Transcription Factors ◽  
Fatty Acid ◽  
Fatty Acid Synthase ◽  
Fatty Acid Synthase Gene

Transcription factors acting on the promoter of the fatty acid synthase gene

2002 ◽  
Vol 30 (5) ◽  
pp. A122-A122
Author(s):  
M. Schweizer ◽  
K. Roder ◽  
L. Zhang ◽  
S.S. Wolf
Keyword(s):  
Transcription Factors ◽  
Fatty Acid ◽  
Fatty Acid Synthase ◽  
Fatty Acid Synthase Gene

Transcriptional regulation of the adipocyte fatty acid synthase gene by agouti: interaction with insulin

2000 ◽  
Vol 3 (3) ◽  
pp. 157-162 ◽  
Author(s):  
KATE J. CLAYCOMBE ◽  
YANXIN WANG ◽  
BRYNN H. JONES ◽  
SUYEON KIM ◽  
WILLIAM O. WILKISON ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Fatty Acid ◽  
Gene Transcription ◽  
Fatty Acid Synthase ◽  
Fusion Gene ◽  
Ectopic Expression ◽  
Fold Increase ◽  
Transcriptional Level ◽  
Additive Effects ◽  
Fatty Acid Synthase Gene

Claycombe, Kate J., Yanxin Wang, Brynn H. Jones, Suyeon Kim, William O. Wilkison, Michael B. Zemel, Joseph Chun, and Naima Moustaid-Moussa. Transcriptional regulation of the adipocyte fatty acid synthase gene by agouti: interaction with insulin. Physiol Genomics 3: 157–162, 2000.—Mice carrying dominant mutations at the agouti locus exhibit ectopic expression of agouti gene transcripts, obesity, and type II diabetes through unknown mechanisms. To gain insight into the role of agouti protein in modulating adiposity, we investigated regulation of a key lipogenic gene, fatty acid synthase (FAS) by agouti alone and in combination with insulin. Both agouti and insulin increase FAS activity in 3T3-L1 and in human adipocytes. Agouti and insulin independently and additively increase FAS activity in 3T3-L1 adipocytes. We further investigated the mechanism responsible for the agouti-induced FAS expression in these cells and demonstrated that both insulin (3-fold increase) and agouti (2-fold) increased FAS gene expression at the transcriptional level. Furthermore, insulin and agouti together exerted additive effects (5-fold increase) on FAS gene transcription. Transfection assays of FAS promoter-luciferase fusion gene constructs into 3T3-L1 adipocytes indicated that the agouti response element(s) is (are) located in the −435 to −415 region (−435/−415) of the FAS promoter. Nuclear proteins binding to this novel sequence are adipocyte specific. Thus the agouti response sequences mapped to a region upstream of the insulin-responsive element (which we previously reported to be located at −67/−52), consistent with additive effects of these two factors on FAS gene transcription.

Involvement of Liver X Receptor Alpha in Histone Modifications Across the Target Fatty Acid Synthase Gene

Lipids ◽  
2011 ◽  
Vol 47 (3) ◽  
pp. 249-257 ◽  
Author(s):  
Huihong Yu ◽  
Jinfeng Wu ◽  
Mei Yang ◽  
Jinjun Guo ◽  
Lili Zheng ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Histone Modifications ◽  
Fatty Acid Synthase ◽  
Liver X Receptor ◽  
Receptor Alpha ◽  
Liver X Receptor Alpha ◽  
Fatty Acid Synthase Gene
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