Effect of a rat plasma high-density lipoprotein subfraction on the synthesis of bile salts by rat hepatocyte monolayers

1985 ◽  
Vol 13 (1) ◽  
pp. 136-137
Author(s):  
ROBERT P. FORD ◽  
KEITH E. SUCKLING ◽  
KATHLEEN M. BOTHAM ◽  
GEORGE S. BOYD
Keyword(s):  
High Density Lipoprotein ◽  
Bile Salts ◽  
Density Lipoprotein ◽  
Rat Plasma ◽  
High Density ◽  
Rat Hepatocyte ◽  
Plasma High Density Lipoprotein ◽  
Plasma High Density ◽  
Lipoprotein Subfraction

A new method for the fractionation of human plasma high density lipoprotein

1977 ◽  
Vol 55 (7) ◽  
pp. 766-768 ◽  
Author(s):  
Bernard Rubenstein ◽  
Susan Evans ◽  
George Steiner
Keyword(s):  
High Density Lipoprotein ◽  
Human Plasma ◽  
Density Lipoprotein ◽  
High Density ◽  
New Method ◽  
Continuous Gradient ◽  
Elution Pattern ◽  
Plasma High Density Lipoprotein ◽  
Plasma High Density ◽  
Peptide Composition

We have devised a new method for the fractionation of human plasma high density lipoprotein (HDL). The HDL was chromatographed on DEAE-agarose columns using a continuous gradient of 0.06–0.15 M NaCl. The elution pattern obtained showed three phases, each with differing peptide composition. Examination of the three subfractions showed that each contained both apo A-I and apo A-II, but indifferent proportions. Subfraction 1 contained noapoC-II orC-III-1 and only a trace of apo C-III-2, subfraction 2 contained apo C-II and C-III-1 but no C-III-2, while subfraction 3 contained considerable apo C-III-2 with only traces of apo C-II or C-III-1.

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