Highly Sensitive Microplate Chemiluminescence Enzyme Immunoassay for the Determination of Staphylococcal Enterotoxin B Based on a Pair of Specific Monoclonal Antibodies and Its Application to Various Matrices

2010 ◽  
Vol 82 (18) ◽  
pp. 7758-7765 ◽  
Author(s):  
Fei Liu ◽  
Yongming Li ◽  
Chaojun Song ◽  
Bangquan Dong ◽  
Zhijia Liu ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Enzyme Immunoassay ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Highly Sensitive ◽  
Enterotoxin B ◽  
Chemiluminescence Enzyme Immunoassay

Development of a Highly Sensitive Chemiluminescence Enzyme Immunoassay for the Determination of Bisphenol A

2013 ◽  
Vol 726-731 ◽  
pp. 1283-1286
Author(s):  
Li Rui Liu ◽  
Li Qin Liu ◽  
Xue Qing Chen ◽  
Guo Qing Shi
Keyword(s):  
Detection Limit ◽  
Bisphenol A ◽  
Enzyme Immunoassay ◽  
Rapid Determination ◽  
Secondary Antibody ◽  
Average Recovery ◽  
Highly Sensitive ◽  
Optimized Conditions ◽  
Chemiluminescence Enzyme Immunoassay

A highly sensitive chemiluminescence enzyme immunoassay (CLEIA) method for the determination of bisphenol A (BPA) was developed, which used a secondary antibody labeled with horseradish peroxidase detected with a luminol-based substrate. Under the optimized conditions, the linear range was 0.01μg/mL~0.74μg/mL, and the detection limit was 0.008μg/mL. The average recovery for BPA in barreled water was 104%. This developed method could be applied for the selective, high-throughput, and rapid determination of BPA in barreled water.

scholarly journals Mechanisms Mediating Enhanced Neutralization Efficacy of Staphylococcal Enterotoxin B by Combinations of Monoclonal Antibodies

2015 ◽  
Vol 290 (11) ◽  
pp. 6715-6730 ◽  
Author(s):  
Kaushik Dutta ◽  
Avanish K. Varshney ◽  
Matthew C. Franklin ◽  
Michael Goger ◽  
Xiaobo Wang ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Enterotoxin B

An ultrasensitive immunosensor array for determination of staphylococcal enterotoxin B

Talanta ◽  
2011 ◽  
Vol 85 (2) ◽  
pp. 1070-1074 ◽  
Author(s):  
Xiaoming Zhang ◽  
Fei Liu ◽  
Ruifang Yan ◽  
Pan Xue ◽  
Yongming Li ◽  
...  
Keyword(s):  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Enterotoxin B

scholarly journals Effective Treatment of Staphylococcal Enterotoxin B Aerosol Intoxication in Rhesus Macaques by Using Two Parenterally Administered High-Affinity Monoclonal Antibodies

2019 ◽  
Vol 63 (5) ◽  
Author(s):  
Daniel Verreault ◽  
Jane Ennis ◽  
Kevin Whaley ◽  
Stephanie Z. Killeen ◽  
Hatice Karauzum ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Nonhuman Primate ◽  
Rhesus Macaques ◽  
Clinical Signs ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Primate Model ◽  
Content Type ◽  
Enterotoxin B

ABSTRACTStaphylococcal enterotoxin B (SEB) is a protein exotoxin found on the cell surface ofStaphylococcus aureusthat is the source for multiple pathologies in humans. When purified and concentrated in aerosol form, SEB can cause an acute and often fatal intoxication and thus is considered a biological threat agent. There are currently no vaccines or treatments approved for human use. Studies with rodent models of SEB intoxication show that antibody therapy may be a promising treatment strategy; however, many have used antibodies only prophylactically or well before any clinical signs of intoxication are apparent. We assessed and compared the protective efficacies of two monoclonal antibodies, Ig121 and c19F1, when administered after aerosol exposure in a uniformly lethal nonhuman primate model of SEB intoxication. Rhesus macaques were challenged using small-particle aerosols of SEB and then were infused intravenously with a single dose of either Ig121 or c19F1 (10 mg/kg of body weight) at either 0.5, 2, or 4 h postexposure. Onset of clinical signs and hematological and cytokine response in untreated controls confirmed the acute onset and potency of the toxin used in the challenge. All animals administered either Ig121 or c19F1 survived SEB challenge, whereas the untreated controls succumbed to SEB intoxication 30 to 48 h postexposure. These results represent the successful therapeuticin vivoprotection by two investigational drugs against SEB in a severe nonhuman primate disease model and punctuate the therapeutic value of monoclonal antibodies when faced with treatment options for SEB-induced toxicity in a postexposure setting.

Triple-Mode Aptasensor for Sensitive and Reliable Determination of Staphylococcal Enterotoxin B

2020 ◽  
Vol 13 (6) ◽  
pp. 1255-1261
Author(s):  
Zhengzong Wu ◽  
Deyun He ◽  
Bo Cui ◽  
Zhengyu Jin ◽  
Enbo Xu
Keyword(s):  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Reliable Determination ◽  
Enterotoxin B ◽  
Triple Mode
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