The effects of some short-chain fatty acids on pyruvate carboxylase activity in intact isolated rat liver mitochondria

1983 ◽  
Vol 11 (3) ◽  
pp. 286-287 ◽  
Author(s):  
JANET-H. STIRK ◽  
KIM BARTLETT ◽  
H. STANLEY A. SHERRATT
Keyword(s):  
Fatty Acids ◽  
Rat Liver ◽  
Pyruvate Carboxylase ◽  
Short Chain Fatty Acids ◽  
Liver Mitochondria ◽  
Short Chain ◽  
Rat Liver Mitochondria ◽  
Carboxylase Activity ◽  
Isolated Rat Liver ◽  
Isolated Rat

scholarly journals A role for 2,4-enoyl-CoA reductase in mitochondrial β-oxidation of polyunsaturated fatty acids. Effects of treatment with clofibrate on oxidation of polyunsaturated acylcarnitines by isolated rat liver mitochondria

1982 ◽  
Vol 208 (3) ◽  
pp. 749-757 ◽  
Author(s):  
H Osmundsen ◽  
J Cervenka ◽  
J Bremer
Keyword(s):  
Fatty Acids ◽  
Rat Liver ◽  
Opposite Effect ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Long Chain Fatty Acids ◽  
Beta Oxidation ◽  
Isolated Rat Liver ◽  
Coa Reductase ◽  
Isolated Rat

1. beta-Oxidation of gamma-linolenoylcarnitine, arachidonoylcarnitine and docosahexaenoylcarnitine by isolated rat liver mitochondria is inhibited by uncoupling conditions. Partial re-activation is obtained with added ATP. With mitochondria from clofibrate-treated rats ATP-stimulated rates of beta-oxidation of docosahexaenoylcarnitine are higher than ADP-stimulated rates. This is not observed with the beta-oxidation of oleoylcarnitine. 2. beta-Oxidation of docosahexaenoylcarnitine, in the presence of rotenone, is inhibited by added oxaloacetate, analogous to previous findings with pent-4-enoylcarnitine [see Osmundsen (1978) FEBS Lett. 88, 219-222]. In the absence of rotenone added oxaloacetate stimulates the beta-oxidation of docosahexaenoylcarnitine, but has the opposite effect on the beta-oxidation of palmitoylcarnitine. 3. beta-Oxidation of polyunsaturated acylcarnitines by isolated rat liver mitochondria is selectively increased after treatment of the animals with a low dietary dose (0.2%, w/w) of clofibrate. Treatment with a higher dose of clofibrate (0.5%, w/w) resulted in a general stimulation of beta-oxidation. 4. The results presented suggest that long-chain fatty acids possessing a delta 4-double bond are not readily beta-oxidized unless the 2,4-enoyl-CoA reductase (EC 1.3.1.-) is operating.

scholarly journals Effect of pent-4-enoic acid, propionic acid and other short-chain fatty acids on citrulline synthesis in rat liver mitochondria

1976 ◽  
Vol 156 (2) ◽  
pp. 301-307 ◽  
Author(s):  
A M Glasgow ◽  
H P Chase
Keyword(s):  
Fatty Acids ◽  
Propionic Acid ◽  
Rat Liver ◽  
Short Chain Fatty Acids ◽  
Liver Mitochondria ◽  
Short Chain ◽  
Enoic Acid ◽  
Rat Liver Mitochondria ◽  
Carbamoyl Phosphate ◽  
Citrulline Synthesis

19 The effect of pent-4-enoic acid, propionic acid and several other short-chain fatty acids on citrulline synthesis in rat liver mitochondria was studied. 2. Pent-4-enoate at 1 mM inhibited mitochondrial citulline synthesis by about 80-90%. It is concluded that pent-4-enoate inhibits citrulline synthesis by interfering with some aspect of mitochondrial energy metabolism. This results in impairment of mitochondrial ornithine uptake or depletion of mitochondrial ATP, which, in turn, impairs carbamoyl phosphate synthesis or both. Evidence in support of this conclusion includes: pent-4-enoate has no effect on citrulline synthesis supported by succinate or exogenous ATP; pent-4-enoate lowers the medium plus mitochondrial ATP concentration; finally, when glutamate is the oxidizable substrate, pent-4-enoate decreases the carbamoyl phosphate concentration in mitochondria incubated without ornithine to minimize citrulline synthesis and impairs the mitochondrial uptake of ornithine, but it has neither effect when succinate is the oxidizable substrate. 4. Propionate, butyrate and crotonate also inhibit mitochondrial citrulline synthesis, but much less than pent-4-enoate. 5. Acetate, pentanoate, pent-2-enoate, hexanoate, octanoate, isovalerate, tiglylate and α-methylbutyrate have little or no effect on mitochondrial citrulline synthesis.

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