The heterogeneity of DNA polymerase-α from mouse embryos and embryonal carcinoma cells

1982 ◽  
Vol 10 (5) ◽  
pp. 348-348
Author(s):  
DUNCAN R. CLARK ◽  
ALEXANDER G. McLENNAN
Keyword(s):  
Dna Polymerase ◽  
Embryonal Carcinoma ◽  
Mouse Embryos ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Dna Polymerase Α

Developmental potential of teratocarcinoma stem cells in utero following aggregation with cleavage-stage mouse embryos

Development ◽  
1983 ◽  
Vol 74 (1) ◽  
pp. 79-96
Author(s):  
Joanne T. Fujii ◽  
Gail R. Martin
Keyword(s):  
Embryonal Carcinoma ◽  
Normal Development ◽  
Mouse Embryos ◽  
Carcinoma Cells ◽  
Abnormal Development ◽  
Clear Correlation ◽  
Embryonal Carcinoma Cells ◽  
Embryonic Cells ◽  
Cleavage Stage ◽  
Developmental Potential

Embryonal carcinoma cells were aggregated with cleavage stage mouse embryos, cultured briefly, and transferred as morulae to the uteri of pseudopregnant females. When midgestation foetuses were examined, many were morphologically abnormal. The severity of this abnormal development was correlated with the extent of contribution by embryonal carcinoma cells to the foetuses as indicated by GPI (glucose phosphate isomerase) analysis. This was true for all three of the cell lines studied, NG-2, PSA-1, and LT1-2D. The clear correlation between increasingly abnormal development and more extensive participation by the embryonal carcinoma cells was not observed in control experiments in which embryos of different stages of development were aggregated together. The data therefore suggest that the embryonal carcinoma cells studied here are unable to support normal development in the absence of a substantial number of host embryonic cells. It remains unclear whether this is a consequence of the karyotypic abnormalities of the cells tested, or whether it reflects a characteristic limitation in the ability of embryonal carcinoma cells to independently direct normal development. When aggregates were allowed to develop to term and the extent of chimaerism was examined in the live-born animals, it was found to be sporadic and limited. This is consistent with the results indicating that large contributions by embryonal carcinoma cells are not compatible with normal development at midgestation. The chimaerism observed in the live-born animals was comparable in both frequency and in tissue distribution to that generally obtained in other studies using either the aggregation or blastocyst injection techniques.

Changes in fucosyl-glycopeptides during early post-implantation embryogenesis in the mouse

Development ◽  
1980 ◽  
Vol 57 (1) ◽  
pp. 25-36
Author(s):  
Takashi Muramatsu ◽  
Hubert Condamine ◽  
Gabriel Gachelin ◽  
François Jacob
Keyword(s):  
Molecular Weight ◽  
Embryonal Carcinoma ◽  
Mouse Embryos ◽  
Carcinoma Cells ◽  
Excluded Volume ◽  
Elution Profile ◽  
Embryonal Carcinoma Cells ◽  
Isolated Organs ◽  
Large Molecular Weight ◽  
Post Implantation

Six-day to 12-day mouse embryos were dissected and radiolabelled by culture in the presence of [3H]fucose. The radiolabelled embryos were extensively digested with Pronase.The resulting glycopeptides were analysed by Sephadex G-50 column chromatography. Glycopeptides from 6-day-old embryos were separated into two main peaks: one eluted near the excluded volume, the other in a well-retarded position. This elution profile was similar to that observed with glycopeptides prepared from embryonal carcinoma cells. The relative amount of the high-molecular-weight glycopeptides decreased during embryonic development and particularly around day 9. Glycopeptide elution profiles from 10-day embryos, or isolated organs of 12-day embryos, were indistinguishable from those obtained from differentiated teratocarcinoma-derived or adult cells. At least 30% of the large molecular weight glycopeptides appear to be located at the cell surface.

The presence of F9 antigen on the surface of mouse embryonic cells until day 8 of embryogenesis

Development ◽  
1978 ◽  
Vol 47 (1) ◽  
pp. 149-160
Author(s):  
M. H. Buc-Caron ◽  
H. Condamine ◽  
F. Jacob
Keyword(s):  
Embryonal Carcinoma ◽  
Germ Line ◽  
Mouse Embryos ◽  
Carcinoma Cells ◽  
Antigenic Determinants ◽  
Embryonal Carcinoma Cells ◽  
Embryonic Cells ◽  
Male Germ Line ◽  
Indirect Immunofluorescence Staining ◽  
Post Implantation

The fate of F9, a surface antigen common to embryonal carcinoma cells and cleavage embryos, as well as to male germ line cells has been studied in post-implantation mouse embryos. The antigen is readily detected on the surface of 7-day and 8-day, but not on 9-day embryonic cells, using anti-F9 serum absorption experiments and indirect immunofluorescence staining. In addition, it is shown that an anti-F9 serum absorbed with 8-day (but not with 9-day) embryonic cells does not react with cleavage embryos (morulae). It is concluded that the antigenic determinants, which are recognized on the surface of morulae by the anti-F9 serum, persist until day 8 of embryogenesis, but are not detected on day 9, either due to their absence, masking or quantitative reduction.

scholarly journals Expression and function of FGF-4 in peri-implantation development in mouse embryos

Development ◽  
1994 ◽  
Vol 120 (8) ◽  
pp. 2259-2269 ◽  
Author(s):  
D.A. Rappolee ◽  
C. Basilico ◽  
Y. Patel ◽  
Z. Werb
Keyword(s):  
Embryonal Carcinoma ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Blastocyst Stage ◽  
Mouse Embryos ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Cell Stage ◽  
And Function

One of the earliest events in mammalian embryogenesis is the formation of the inner cell mass (ICM) and the subsequent delamination of primitive endoderm. We have found that mRNA for fibroblast growth factor (FGF)-4, but not FGF-3, is expressed in preimplantation mouse blastocysts and that the FGF-4 polypeptide is present in ICM cells. ICM-like embryonal carcinoma cells and embryonic stem cells also express FGF-4. Conversely, differentiated embryonal carcinoma cells in the endoderm lineage express FGF-3, but not FGF-4 mRNA. Although mouse embryos expressed FGF-4 mRNA from the 1-cell stage, embryos cultured from the 2-cell through the blastocyst stage in the presence of recombinant FGF-4 did not respond mitogenically. However, when ICMs that were isolated by immunosurgery were cultured with FGF-4, the number of morphologically distinct, differentiated parietal endoderm cells growing out onto the coverslip increased, without an increase in the number of undifferentiated ICM cells. ICM outgrowths cultured with FGF-4 increased their secretion of 92 × 10(3) M(r) gelatinase and tissue plasminogen activator, a hallmark of migrating cells. Receptors for FGF-4 (FGFR-3 and FGFR-4) are expressed in all cells of the mouse blastocyst. These findings indicate that FGF-4 produced by undifferentiated ICM cells acts in the peri-implantation period of embryogenesis to influence the production and behavior of endoderm cells derived from them.

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