Demethylation in the 5′-flanking region of mouse cellular retinoic acid binding protein-I gene is associated with its high level of expression in mouse embryos and facilitates its induction by retinoic acid in P19 embryonal carcinoma cells

1994 ◽  
Vol 201 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Li-Na Wei ◽  
Chih-Hao Lee
Keyword(s):  
Retinoic Acid ◽  
Embryonal Carcinoma ◽  
Mouse Embryos ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Acid Binding Protein ◽  
P19 Embryonal Carcinoma Cells ◽  
Flanking Region ◽  
High Level ◽  
I Gene

Aggregation and cell cycle dependent retinoic acid receptor mRNA expression in P19 embryonal carcinoma cells

1992 ◽  
Vol 36 (3) ◽  
pp. 165-172 ◽  
Author(s):  
Luigi J.C. Jonk ◽  
Marjolijn E.J. de Jonge ◽  
Frank A.E. Kruyt ◽  
Christine L. Mummery ◽  
Paul T. van der Saag ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Retinoic Acid ◽  
Mrna Expression ◽  
Embryonal Carcinoma ◽  
Retinoic Acid Receptor ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Receptor Mrna ◽  
P19 Embryonal Carcinoma Cells ◽  
Cycle Dependent

scholarly journals Sequence and expression pattern of the Stra7 (Gbx-2) homeobox-containing gene induced by retinoic acid in P19 embryonal carcinoma cells

1995 ◽  
Vol 204 (4) ◽  
pp. 372-382 ◽  
Author(s):  
Philippe Bouillet ◽  
Claire Chazaud ◽  
Mustapha Oulad-Abdelghani ◽  
Pascal Dollé ◽  
Pierre Chambon
Keyword(s):  
Retinoic Acid ◽  
Expression Pattern ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
P19 Embryonal Carcinoma Cells

Modulation of the Retinoic Acid and Retinoid X Receptor Signaling Pathways in P19 Embryonal Carcinoma Cells by Calreticulin

1997 ◽  
Vol 230 (1) ◽  
pp. 50-60 ◽  
Author(s):  
Mary Shago ◽  
Grace Flock ◽  
Chung-Yee Leung Hagesteijn ◽  
Michael Woodside ◽  
Sergio Grinstein ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Signaling Pathways ◽  
Embryonal Carcinoma ◽  
Retinoid X Receptor ◽  
Carcinoma Cells ◽  
Receptor Signaling ◽  
Embryonal Carcinoma Cells ◽  
P19 Embryonal Carcinoma Cells

scholarly journals Induction and repression of mammalian achaete-scute homologue (MASH) gene expression during neuronal differentiation of P19 embryonal carcinoma cells

Development ◽  
1992 ◽  
Vol 114 (1) ◽  
pp. 75-87 ◽  
Author(s):  
J.E. Johnson ◽  
K. Zimmerman ◽  
T. Saito ◽  
D.J. Anderson
Keyword(s):  
Retinoic Acid ◽  
Neuronal Differentiation ◽  
Embryonal Carcinoma ◽  
Model System ◽  
Neuronal Morphology ◽  
Carcinoma Cells ◽  
P19 Cells ◽  
Embryonal Carcinoma Cells ◽  
Bhlh Genes ◽  
P19 Embryonal Carcinoma Cells

MASH1 and MASH2, mammalian homologues of the Drosophila neural determination genes achaete-scute, are members of the basic helix-loop-helix (bHLH) family of transcription factors. We show here that murine P19 embryonal carcinoma cells can be used as a model system to study the regulation and function of these genes. MASH1 and MASH2 display complementary patterns of expression during the retinoic-acid-induced neuronal differentiation of P19 cells. MASH1 mRNA is undetectable in undifferentiated P19 cells but is induced to high levels by retinoic acid coincident with neuronal differentiation. In contrast, MASH2 mRNA is expressed in undifferentiated P19 cells and is repressed by retinoic acid treatment. These complementary expression patterns suggest distinct functions for MASH1 and MASH2 in development, despite their sequence homology. In retinoic-acid-treated P19 cells, MASH1 protein expression precedes and then overlaps expression of neuronal markers. However, MASH1 is expressed by a smaller proportion of cells than expresses such markers. MASH1 immunoreactivity is not detected in differentiated cells displaying a neuronal morphology, suggesting that its expression is transient. These features of MASH1 expression are similar to those observed in vivo, and suggest that P19 cells represent a good model system in which to study the regulation of this gene. Forced expression of MASH1 was achieved in undifferentiated P19 cells by transfection of a cDNA expression construct. The transfected cells expressing exogenous MASH1 protein contained E-box-binding activity that could be super-shifted by an anti-MASH1 antibody, but exhibited no detectable phenotypic changes. Thus, unlike myogenic bHLH genes, such as MyoD, which are sufficient to induce muscle differentiation, expression of MASH1 appears insufficient to promote neurogenesis.

Expression of Pax-3- and neuroectoderm-inducing activities during differentiation of P19 embryonal carcinoma cells

Development ◽  
1992 ◽  
Vol 116 (3) ◽  
pp. 573-583 ◽  
Author(s):  
S.C. Pruitt
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Embryonal Carcinoma ◽  
Fusion Gene ◽  
Carcinoma Cells ◽  
Target Cells ◽  
Lacz Gene ◽  
Cellular Interactions ◽  
Embryonal Carcinoma Cells ◽  
P19 Embryonal Carcinoma Cells

A P19 embryonal carcinoma stem cell line carrying an insertion of the E. coli LacZ gene in an endogenous copy of the Pax-3 gene was identified. Expression of the Pax-3/LacZ fusion gene in neuroectodermal and mesodermal lineages following induction of differentiation by chemical treatments (retinoic acid and dimethylsulfoxide) was characterized using this line and is consistent with the previous localization of Pax-3 expression in the embryo to mitotically active cells of the dorsal neuroectoderm and the adjacent segmented dermomyotome. Pax-3/LacZ marked stem cells were also utilized as target cells in mixing experiments with unmarked P19 cells that had been differentiated by pretreatment with chemical inducers. Induction of beta-galactosidase and neuroectodermal markers in the target cells demonstrates that: (1) some differentiated P19 cell derivatives transiently express endogenous Pax-3- and neuroectoderm-inducing activities, (2) undifferentiated target stem cells respond to these activities even in the presence of leukemia inhibitory factor and (3) the endogenous activities can be distinguished from, and are more potent than, retinoic acid treatment in inducing neuroectoderm. These observations demonstrate that P19 embryonal carcinoma cells provide a useful in vitro system for analysis of the cellular interactions responsible for neuroectoderm induction in mammals.

Transient expression of the proto-oncogene int-1 during differentiation of P19 embryonal carcinoma cells

1989 ◽  
Vol 9 (3) ◽  
pp. 1357-1361
Author(s):  
E Schuuring ◽  
L van Deemter ◽  
H Roelink ◽  
R Nusse
Keyword(s):  
Retinoic Acid ◽  
Neural Development ◽  
Time Course ◽  
Embryonal Carcinoma ◽  
Dose Dependence ◽  
Carcinoma Cells ◽  
P19 Cells ◽  
Embryonal Carcinoma Cells ◽  
P19 Embryonal Carcinoma Cells

In mouse embryos, the int-1 proto-oncogene is transiently expressed in areas of the developing neural system. Retinoic acid-treated P19 embryonal carcinoma cells have often been used as an in vitro model for the molecular basis of neural development. We shown here that int-1 is transiently expressed in differentiated P19 cells. The time course and retinoic acid dose dependence of int-1 expression suggest that the gene is specifically expressed during early neural differentiation. P19 cells may be a useful model to assist in the study, at the cellular level, of the role of int-1 in neural development.

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