Transfer of nucleotide regulatory protein from human erythrocytes to desensitized turkey erythrocytes by cell fusion restores β-catecholamine responsiveness

1982 ◽  
Vol 10 (1) ◽  
pp. 52-53
Author(s):  
D. MICHAEL SALMON ◽  
CLAIRE WILSON ◽  
DENNIS SCHULSTER
Keyword(s):  
Cell Fusion ◽  
Regulatory Protein ◽  
Human Erythrocytes ◽  
Catecholamine Responsiveness

scholarly journals Calcium ions and cell fusion. Effects of chemical fusogens on the permeability of erythrocytes to calcium and other ions

1979 ◽  
Vol 182 (2) ◽  
pp. 555-563 ◽  
Author(s):  
A M J Blow ◽  
G M Botham ◽  
J A Lucy
Keyword(s):  
Cell Fusion ◽  
Calcium Ions ◽  
Biological Membranes ◽  
Human Erythrocytes ◽  
Water Soluble ◽  
Chemically Induced

1. Fusogenic and non-fusogenic chemicals were tesetd for their ability to allow 45Ca2+ and 3H2O to enter hen and human erythrocytes. 2. The ratio of 45Ca2+/3H2O in treated cells to that in untreated cells is referred to as the entry ratio. 3. Within 1 min at 37 degrees C both water-soluble and lipid-soluble fusogens increased the value of the entry ratio, which reached maximum values in 5–10 min. 4. Values of the entry ratio in the range of 4–12 were found under conditions that led to cell fusion. 5. Closely related but non-fusogenic chemicals did not significantly alter the entry ratio. 6. The entry ratios for 86Rb+, 22Na+ and 35SO42- were also significantly increased by both lipid-soluble and water-soluble fusogens, though the increases were not as large as those for 45Ca2+. 7. It is suggested that fusogenic compounds increase the permeability of biological membranes to ions, and that an increase in the concentration of intracellular Ca2+ initiates or facilitates events that lead to the chemically induced fusion of erythrocytes.

Regulation of human immunodeficiency virus gp160-mediated cell fusion by antibodies against fusion regulatory protein 1

1996 ◽  
Vol 77 (11) ◽  
pp. 2747-2756 ◽  
Author(s):  
S. Ohgimoto ◽  
N. Tabata ◽  
S. Suga ◽  
M. Tsurudome ◽  
M. Kawano ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Cell Fusion ◽  
Regulatory Protein ◽  
Immunodeficiency Virus

scholarly journals "Ultramicroinjection" of macromolecules or small particles into animal cells. A new technique based on virus-induced cell fusion.

1975 ◽  
Vol 66 (2) ◽  
pp. 292-304 ◽  
Author(s):  
A Loyter ◽  
N Zakai ◽  
R G Kulka
Keyword(s):  
Cell Fusion ◽  
Sendai Virus ◽  
Human Erythrocytes ◽  
New Method ◽  
New Technique ◽  
Second Step ◽  
Small Particles ◽  
Animal Cells ◽  
Latex Spheres ◽  
A New Technique

A new method is described for the introduction of macromolecules and small particles into animal cells. The first step in this procedure is the trapping of particles in ghosts of human erythrocytes. This is achieved by the gradual hemolysis of erythrocytes in the presence of the particles to be trapped. The second step is the Sendai virus-induced fusion of the ghosts containing the particles with cells. By this method, ferritin and latex spheres (diameter 0.1 mum) have been "injected" into cells.

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