scholarly journals "Ultramicroinjection" of macromolecules or small particles into animal cells. A new technique based on virus-induced cell fusion.

1975 ◽  
Vol 66 (2) ◽  
pp. 292-304 ◽  
Author(s):  
A Loyter ◽  
N Zakai ◽  
R G Kulka

A new method is described for the introduction of macromolecules and small particles into animal cells. The first step in this procedure is the trapping of particles in ghosts of human erythrocytes. This is achieved by the gradual hemolysis of erythrocytes in the presence of the particles to be trapped. The second step is the Sendai virus-induced fusion of the ghosts containing the particles with cells. By this method, ferritin and latex spheres (diameter 0.1 mum) have been "injected" into cells.

1984 ◽  
Vol 247 (6) ◽  
pp. H1018-H1026 ◽  
Author(s):  
G. Bkaily ◽  
N. Sperelakis ◽  
J. Doane

A new technique for isolation of single cardiac cells from adult rat ventricles is described. The mechanical method is modified from the method of Jacobson (Cell Struct. Funct. 2: 1-9, 1977) by employing an enzyme-free dispersion medium containing Sr2+ or Ba2+ to replace Ca2+. This method produces a high yield of isolated rod-shaped adult mammalian cardiomyocytes that do not contract spontaneously, have a normal resting potential, and are Ca2+ tolerant. This method represents a promising technique for isolation of single, viable, intact, adult myocytes.


2004 ◽  
Vol 92 (4-6) ◽  
Author(s):  
K. Franke ◽  
J. T. Patt ◽  
M. Patt ◽  
H. Kupsch ◽  
J. Steinbach

AbstractA new method of radiolabelling of humic substances (HS) in the aqueous phase has been developed. Radiolabelling with the short-lived positron-emitter


1990 ◽  
Vol 2 (6) ◽  
pp. 333-337 ◽  
Author(s):  
R.D. Ashpole ◽  
H. Snyman ◽  
J.A. Bullimore ◽  
H.J. Appleby ◽  
B.H. Cummins ◽  
...  

2011 ◽  
Vol 2 (3) ◽  
pp. 85-90
Author(s):  
A V Safin ◽  
O P Kuzovlev

The article is devoted to a new technique in neurosurgery – spinal cord neurostimulation. It has also focused on the patophysiology of pain and spastic syndromes. Surgical treatment of pain, spasti-city and stages of neurostimulation are also considered.


2020 ◽  
Vol 7 (4) ◽  
pp. 631-641
Author(s):  
Abdul-Sattar J. Al-Saif ◽  
Takia Ahmed J. Al-Griffi

In this research, we have proposed a new technique to solve two-dimensional (2D) viscous fluid flow among slowly expanding or contracting walls. The new technique depends on combining the algorithms of Yang transform and the homotopy perturbation methods. The results, obtained from the first iteration and by using the new method, show the accuracy and efficiency of this method compared to the other methods, used to find the analytical approximate solution for the problem caused by the 2D viscous fluid flow. Moreover, the graphs of the new solutions show the validity, usefulness and necessity of the new method.


2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Syed Inayatullah ◽  
Maria Aman ◽  
Asma Rani ◽  
Hina Zaheer ◽  
Tanveer Ahmed Siddiqi

In this article, we have presented a method for finding the approximate center of a linear programming polytope. This method provides a point near the center of a polytope in few simple and easy steps. Geometrical interpretation and some numerical examples have also been presented to demonstrate the proposed approach and comparison of quality of the center obtained by using the new method with existing methods of finding exact and approximate centers. At the end, we also presented computational results on the randomly generated polytopes to compare the quality of the center obtained by using the new method.


1950 ◽  
Vol 27 (4) ◽  
pp. 378-382
Author(s):  
George Gallup

Twenty years ago the QUARTERLY published an article by Dr. Gallup on “A Scientific Method for Determining Reader-Interest.” He now describes a new technique which he predicts will change the character of advertising as much in the next 20 years as the readership method has changed it in the last 20.


1995 ◽  
Vol 3 (2) ◽  
pp. 23-32
Author(s):  
Mark D Wells ◽  
Scott Oishi ◽  
Mustafa Sengezer

A new technique is described for stabilizing complex vertical fractures of the maxilla. Advantageous use of the comminuted fracture pattern of the maxillary buttresses allows the maxilla to be divided into two parts at the Le Fort I level. with intermaxillary fixation applied, stabilization is achieved by applying miniplate fixation to the nasal side of the hard palate. The maxilla is reduced to the previously stabilized anterior midfacial buttresses with plates and screws. Comminuted segments of defects in the anterior buttresses are replaced with contoured calvarial grafts. This method has the advantage of maintaining transverse palatal width in a rigid fashion without the need for further osteotomies. It has satisfactorily restored preinjury occlusal relationships in six patients, without the need for dental splints. In no instance has hardware extrusion occurred after fixation.


1972 ◽  
Vol 43 (10) ◽  
pp. 1456-1459 ◽  
Author(s):  
S. Temkin ◽  
J. M. Reichman

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